{"doi":"10.1016/j.devcel.2015.12.013","author":[{"last_name":"Sedzinski","full_name":"Sedzinski, Jakub","first_name":"Jakub"},{"orcid":"0000-0001-6005-1561","first_name":"Edouard B","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo"},{"first_name":"Fan","full_name":"Tu, Fan","last_name":"Tu"},{"last_name":"Biro","first_name":"Maté","full_name":"Biro, Maté"},{"last_name":"Wallingford","first_name":"John","full_name":"Wallingford, John"}],"_id":"932","title":"Emergence of an Apical Epithelial Cell Surface In Vivo","publication":"Developmental Cell","publication_status":"published","issue":"1","abstract":[{"text":"Epithelial sheets are crucial components of all metazoan animals, enclosing organs and protecting the animal from its environment. Epithelial homeostasis poses unique challenges, as addition of new cells and loss of old cells must be achieved without disrupting the fluid-tight barrier and apicobasal polarity of the epithelium. Several studies have identified cell biological mechanisms underlying extrusion of cells from epithelia, but far less is known of the converse mechanism by which new cells are added. Here, we combine molecular, pharmacological, and laser-dissection experiments with theoretical modeling to characterize forces driving emergence of an apical surface as single nascent cells are added to a vertebrate epithelium in vivo. We find that this process involves the interplay between cell-autonomous actin-generated pushing forces in the emerging cell and mechanical properties of neighboring cells. Our findings define the forces driving this cell behavior, contributing to a more comprehensive understanding of epithelial homeostasis.","lang":"eng"}],"oa_version":"None","volume":36,"publist_id":"6510","date_updated":"2021-01-12T08:22:00Z","type":"journal_article","extern":"1","intvolume":"        36","language":[{"iso":"eng"}],"page":"24 - 35","publisher":"Cell Press","year":"2016","status":"public","day":"12","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2016-01-12T00:00:00Z","article_processing_charge":"No","acknowledgement":"We thank J. Bear, B. Goldstein, A. Ewald, and D. Soroldoni for critical reading. This work was funded by an EMBO Long Term Fellowship to J.S., a Research Fellowship from Trinity College, Cambridge and a Bettencourt-Schueller Foundation Young Researcher Prize to E.H., a Cancer Institute NSW Early Career Researcher fellowship (13/ECF/1–25) and a Cancer Australia/Cure Cancer Australia Foundation project grant (1070498) to M.B., and grants from the NHLBI (HL117164) and NIGMS (GM074104) to J.B.W. J.B.W. was an early career scientist of the Howard Hughes Medical Institute. This work was initiated at the New Quantitative Approaches to Morphogenesis Workshop at UCSB, which is funded in part by the National Science Foundation (PHY11-25915) and the NIGMS (GM067110-05).","citation":{"short":"J. Sedzinski, E.B. Hannezo, F. Tu, M. Biro, J. Wallingford, Developmental Cell 36 (2016) 24–35.","ama":"Sedzinski J, Hannezo EB, Tu F, Biro M, Wallingford J. Emergence of an Apical Epithelial Cell Surface In Vivo. <i>Developmental Cell</i>. 2016;36(1):24-35. doi:<a href=\"https://doi.org/10.1016/j.devcel.2015.12.013\">10.1016/j.devcel.2015.12.013</a>","mla":"Sedzinski, Jakub, et al. “Emergence of an Apical Epithelial Cell Surface In Vivo.” <i>Developmental Cell</i>, vol. 36, no. 1, Cell Press, 2016, pp. 24–35, doi:<a href=\"https://doi.org/10.1016/j.devcel.2015.12.013\">10.1016/j.devcel.2015.12.013</a>.","ieee":"J. Sedzinski, E. B. Hannezo, F. Tu, M. Biro, and J. Wallingford, “Emergence of an Apical Epithelial Cell Surface In Vivo,” <i>Developmental Cell</i>, vol. 36, no. 1. Cell Press, pp. 24–35, 2016.","chicago":"Sedzinski, Jakub, Edouard B Hannezo, Fan Tu, Maté Biro, and John Wallingford. “Emergence of an Apical Epithelial Cell Surface In Vivo.” <i>Developmental Cell</i>. Cell Press, 2016. <a href=\"https://doi.org/10.1016/j.devcel.2015.12.013\">https://doi.org/10.1016/j.devcel.2015.12.013</a>.","ista":"Sedzinski J, Hannezo EB, Tu F, Biro M, Wallingford J. 2016. Emergence of an Apical Epithelial Cell Surface In Vivo. Developmental Cell. 36(1), 24–35.","apa":"Sedzinski, J., Hannezo, E. B., Tu, F., Biro, M., &#38; Wallingford, J. (2016). Emergence of an Apical Epithelial Cell Surface In Vivo. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2015.12.013\">https://doi.org/10.1016/j.devcel.2015.12.013</a>"},"date_created":"2018-12-11T11:49:16Z","month":"01"}