{"acknowledgement":"We are grateful to Andrey Poltaraus and his colleagues for sequencing a/eRF1 genes. We thank Tatyana Pestova and Chris Hellen for the gift of plasmids encoding initiation factors eIF1, eIF1A, eIF4A, eIF4B, eIF4G, eIF5, eIF5B, and Anna Yaremchuk and Michael Tukalo for M. jannaschii aRF1. This work was supported by grants from the Presidium of the (Program Molecular and Cell Biology), the Russian Foundation for Basic Research (08-04-01091-а to E.A. and 08-04-00375a to L.F.), the National Institute for General Medical Sciences (to D.S.), the National Science Foundation (to D.S.) and the Office of Basic Energy Sciences, DOE (to D.S.).","citation":{"ieee":"E. Alkalaeva <i>et al.</i>, “Translation termination in pyrrolysine-utilizing archaea,” <i>FEBS Letters</i>, vol. 583, no. 21. Elsevier, pp. 3455–3460, 2009.","short":"E. Alkalaeva, B. Eliseev, A. Ambrogelly, P. Vlasov, F. Kondrashov, S. Gundllapalli, L. Frolova, D. Söll, L. Kisselev, FEBS Letters 583 (2009) 3455–3460.","mla":"Alkalaeva, Elena, et al. “Translation Termination in Pyrrolysine-Utilizing Archaea.” <i>FEBS Letters</i>, vol. 583, no. 21, Elsevier, 2009, pp. 3455–60, doi:<a href=\"https://doi.org/10.1016/j.febslet.2009.09.044\">10.1016/j.febslet.2009.09.044</a>.","ama":"Alkalaeva E, Eliseev B, Ambrogelly A, et al. Translation termination in pyrrolysine-utilizing archaea. <i>FEBS Letters</i>. 2009;583(21):3455-3460. doi:<a href=\"https://doi.org/10.1016/j.febslet.2009.09.044\">10.1016/j.febslet.2009.09.044</a>","apa":"Alkalaeva, E., Eliseev, B., Ambrogelly, A., Vlasov, P., Kondrashov, F., Gundllapalli, S., … Kisselev, L. (2009). Translation termination in pyrrolysine-utilizing archaea. <i>FEBS Letters</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.febslet.2009.09.044\">https://doi.org/10.1016/j.febslet.2009.09.044</a>","ista":"Alkalaeva E, Eliseev B, Ambrogelly A, Vlasov P, Kondrashov F, Gundllapalli S, Frolova L, Söll D, Kisselev L. 2009. Translation termination in pyrrolysine-utilizing archaea. FEBS Letters. 583(21), 3455–3460.","chicago":"Alkalaeva, Elena, Boris Eliseev, Alexandre Ambrogelly, Peter Vlasov, Fyodor Kondrashov, Sarath Gundllapalli, Ludmila Frolova, Dieter Söll, and Lev Kisselev. “Translation Termination in Pyrrolysine-Utilizing Archaea.” <i>FEBS Letters</i>. Elsevier, 2009. <a href=\"https://doi.org/10.1016/j.febslet.2009.09.044\">https://doi.org/10.1016/j.febslet.2009.09.044</a>."},"month":"11","date_created":"2018-12-11T11:49:08Z","publisher":"Elsevier","status":"public","year":"2009","page":"3455 - 3460","day":"03","date_published":"2009-11-03T00:00:00Z","publist_id":"6740","date_updated":"2021-01-12T08:21:49Z","volume":583,"extern":1,"type":"journal_article","intvolume":"       583","_id":"908","doi":"10.1016/j.febslet.2009.09.044","author":[{"last_name":"Alkalaeva","first_name":"Elena","full_name":"Alkalaeva, Elena Z"},{"first_name":"Boris","full_name":"Eliseev, Boris D","last_name":"Eliseev"},{"last_name":"Ambrogelly","first_name":"Alexandre","full_name":"Ambrogelly, Alexandre"},{"first_name":"Peter","full_name":"Vlasov, Peter K","last_name":"Vlasov"},{"full_name":"Fyodor Kondrashov","first_name":"Fyodor","orcid":"0000-0001-8243-4694","last_name":"Kondrashov","id":"44FDEF62-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Gundllapalli","first_name":"Sarath","full_name":"Gundllapalli, Sarath B"},{"last_name":"Frolova","full_name":"Frolova, Ludmila Y","first_name":"Ludmila"},{"first_name":"Dieter","full_name":"Söll, Dieter G","last_name":"Söll"},{"last_name":"Kisselev","full_name":"Kisselev, Lev L","first_name":"Lev"}],"quality_controlled":0,"title":"Translation termination in pyrrolysine-utilizing archaea","publication_status":"published","issue":"21","publication":"FEBS Letters","abstract":[{"lang":"eng","text":"Although some data link archaeal and eukaryotic translation, the overall mechanism of protein synthesis in archaea remains largely obscure. Both archaeal (aRF1) and eukaryotic (eRF1) single release factors recognize all three stop codons. The archaeal genus Methanosarcinaceae contains two aRF1 homologs, and also uses the UAG stop to encode the 22nd amino acid, pyrrolysine. Here we provide an analysis of the last stage of archaeal translation in pyrrolysine-utilizing species. We demonstrated that only one of two Methanosarcina barkeri aRF1 homologs possesses activity and recognizes all three stop codons. The second aRF1 homolog may have another unknown function. The mechanism of pyrrolysine incorporation in the Methanosarcinaceae is discussed."}]}