{"tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"day":"01","publisher":"BioMed Central","year":"2012","status":"public","license":"https://creativecommons.org/licenses/by/4.0/","date_published":"2012-09-01T00:00:00Z","acknowledgement":"We thank Elena Alkalaeva and Peter Kolosov for insightful discussion and Brian Charlesworth for a critical reading of our manuscript. The work has been supported by a Plan Nacional grant from the Spanish Ministry of Science and Innovation, EMBO Young Investigator and Howard Hughes Medical Institute International Early Career Scientist awards.\n","month":"09","date_created":"2018-12-11T11:48:52Z","citation":{"ama":"Povolotskaya I, Kondrashov F, Ledda A, Vlasov P. Stop codons in bacteria are not selectively equivalent. <i>Biology Direct</i>. 2012;7. doi:<a href=\"https://doi.org/10.1186/1745-6150-7-30\">10.1186/1745-6150-7-30</a>","mla":"Povolotskaya, Inna, et al. “Stop Codons in Bacteria Are Not Selectively Equivalent.” <i>Biology Direct</i>, vol. 7, BioMed Central, 2012, doi:<a href=\"https://doi.org/10.1186/1745-6150-7-30\">10.1186/1745-6150-7-30</a>.","short":"I. Povolotskaya, F. Kondrashov, A. Ledda, P. Vlasov, Biology Direct 7 (2012).","ieee":"I. Povolotskaya, F. Kondrashov, A. Ledda, and P. Vlasov, “Stop codons in bacteria are not selectively equivalent,” <i>Biology Direct</i>, vol. 7. BioMed Central, 2012.","ista":"Povolotskaya I, Kondrashov F, Ledda A, Vlasov P. 2012. Stop codons in bacteria are not selectively equivalent. Biology Direct. 7.","chicago":"Povolotskaya, Inna, Fyodor Kondrashov, Alice Ledda, and Peter Vlasov. “Stop Codons in Bacteria Are Not Selectively Equivalent.” <i>Biology Direct</i>. BioMed Central, 2012. <a href=\"https://doi.org/10.1186/1745-6150-7-30\">https://doi.org/10.1186/1745-6150-7-30</a>.","apa":"Povolotskaya, I., Kondrashov, F., Ledda, A., &#38; Vlasov, P. (2012). Stop codons in bacteria are not selectively equivalent. <i>Biology Direct</i>. BioMed Central. <a href=\"https://doi.org/10.1186/1745-6150-7-30\">https://doi.org/10.1186/1745-6150-7-30</a>"},"quality_controlled":0,"title":"Stop codons in bacteria are not selectively equivalent","_id":"858","doi":"10.1186/1745-6150-7-30","author":[{"last_name":"Povolotskaya","full_name":"Povolotskaya, Inna","first_name":"Inna"},{"id":"44FDEF62-F248-11E8-B48F-1D18A9856A87","last_name":"Kondrashov","orcid":"0000-0001-8243-4694","full_name":"Fyodor Kondrashov","first_name":"Fyodor"},{"last_name":"Ledda","first_name":"Alice","full_name":"Ledda, Alice"},{"last_name":"Vlasov","full_name":"Vlasov, Peter K","first_name":"Peter"}],"abstract":[{"lang":"eng","text":"ackground: The evolution and genomic stop codon frequencies have not been rigorously studied with the exception of coding of non-canonical amino acids. Here we study the rate of evolution and frequency distribution of stop codons in bacterial genomes.Results: We show that in bacteria stop codons evolve slower than synonymous sites, suggesting the action of weak negative selection. However, the frequency of stop codons relative to genomic nucleotide content indicated that this selection regime is not straightforward. The frequency of TAA and TGA stop codons is GC-content dependent, with TAA decreasing and TGA increasing with GC-content, while TAG frequency is independent of GC-content. Applying a formal, analytical model to these data we found that the relationship between stop codon frequencies and nucleotide content cannot be explained by mutational biases or selection on nucleotide content. However, with weak nucleotide content-dependent selection on TAG, -0.5 < Nes < 1.5, the model fits all of the data and recapitulates the relationship between TAG and nucleotide content. For biologically plausible rates of mutations we show that, in bacteria, TAG stop codon is universally associated with lower fitness, with TAA being the optimal for G-content < 16% while for G-content > 16% TGA has a higher fitness than TAG.Conclusions: Our data indicate that TAG codon is universally suboptimal in the bacterial lineage, such that TAA is likely to be the preferred stop codon for low GC content while the TGA is the preferred stop codon for high GC content. The optimization of stop codon usage may therefore be useful in genome engineering or gene expression optimization applications.Reviewers: This article was reviewed by Michail Gelfand, Arcady Mushegian and Shamil Sunyaev. For the full reviews, please go to the Reviewers' Comments section."}],"publication_status":"published","publication":"Biology Direct","extern":1,"type":"journal_article","date_updated":"2021-01-12T08:20:08Z","publist_id":"6792","volume":7,"intvolume":"         7"}