{"volume":13,"date_updated":"2021-01-12T08:19:16Z","type":"journal_article","extern":"1","intvolume":"        13","publication_identifier":{"issn":["1554-8929","1554-8937"]},"language":[{"iso":"eng"}],"doi":"10.1021/acschembio.8b00271","author":[{"full_name":"Laguri, Cedric","first_name":"Cedric","last_name":"Laguri"},{"first_name":"Alba","full_name":"Silipo, Alba","last_name":"Silipo"},{"full_name":"Martorana, Alessandra M.","first_name":"Alessandra M.","last_name":"Martorana"},{"id":"7B541462-FAF6-11E9-A490-E8DFE5697425","last_name":"Schanda","orcid":"0000-0002-9350-7606","full_name":"Schanda, Paul","first_name":"Paul"},{"last_name":"Marchetti","full_name":"Marchetti, Roberta","first_name":"Roberta"},{"full_name":"Polissi, Alessandra","first_name":"Alessandra","last_name":"Polissi"},{"full_name":"Molinaro, Antonio","first_name":"Antonio","last_name":"Molinaro"},{"full_name":"Simorre, Jean-Pierre","first_name":"Jean-Pierre","last_name":"Simorre"}],"_id":"8439","title":"Solid state NMR studies of intact lipopolysaccharide endotoxin","quality_controlled":"1","issue":"8","publication":"ACS Chemical Biology","publication_status":"published","abstract":[{"lang":"eng","text":"Lipopolysaccharides (LPS) are complex glycolipids forming the outside layer of Gram-negative bacteria. Their hydrophobic and heterogeneous nature greatly hampers their structural study in an environment similar to the bacterial surface. We have studied LPS purified from E. coli and pathogenic P. aeruginosa with long O-antigen polysaccharides assembled in solution as vesicles or elongated micelles. Solid-state NMR with magic-angle spinning permitted the identification of NMR signals arising from regions with different flexibilities in the LPS, from the lipid components to the O-antigen polysaccharides. Atomic scale data on the LPS enabled the study of the interaction of gentamicin antibiotic bound to P. aeruginosa LPS, for which we could confirm that a specific oligosaccharide is involved in the antibiotic binding. The possibility to study LPS alone and bound to a ligand when it is assembled in membrane-like structures opens great prospects for the investigation of proteins and antibiotics that specifically target such an important molecule at the surface of Gram-negative bacteria."}],"oa_version":"None","article_processing_charge":"No","citation":{"ama":"Laguri C, Silipo A, Martorana AM, et al. Solid state NMR studies of intact lipopolysaccharide endotoxin. <i>ACS Chemical Biology</i>. 2018;13(8):2106-2113. doi:<a href=\"https://doi.org/10.1021/acschembio.8b00271\">10.1021/acschembio.8b00271</a>","mla":"Laguri, Cedric, et al. “Solid State NMR Studies of Intact Lipopolysaccharide Endotoxin.” <i>ACS Chemical Biology</i>, vol. 13, no. 8, American Chemical Society, 2018, pp. 2106–13, doi:<a href=\"https://doi.org/10.1021/acschembio.8b00271\">10.1021/acschembio.8b00271</a>.","short":"C. Laguri, A. Silipo, A.M. Martorana, P. Schanda, R. Marchetti, A. Polissi, A. Molinaro, J.-P. Simorre, ACS Chemical Biology 13 (2018) 2106–2113.","ieee":"C. Laguri <i>et al.</i>, “Solid state NMR studies of intact lipopolysaccharide endotoxin,” <i>ACS Chemical Biology</i>, vol. 13, no. 8. American Chemical Society, pp. 2106–2113, 2018.","chicago":"Laguri, Cedric, Alba Silipo, Alessandra M. Martorana, Paul Schanda, Roberta Marchetti, Alessandra Polissi, Antonio Molinaro, and Jean-Pierre Simorre. “Solid State NMR Studies of Intact Lipopolysaccharide Endotoxin.” <i>ACS Chemical Biology</i>. American Chemical Society, 2018. <a href=\"https://doi.org/10.1021/acschembio.8b00271\">https://doi.org/10.1021/acschembio.8b00271</a>.","ista":"Laguri C, Silipo A, Martorana AM, Schanda P, Marchetti R, Polissi A, Molinaro A, Simorre J-P. 2018. Solid state NMR studies of intact lipopolysaccharide endotoxin. ACS Chemical Biology. 13(8), 2106–2113.","apa":"Laguri, C., Silipo, A., Martorana, A. M., Schanda, P., Marchetti, R., Polissi, A., … Simorre, J.-P. (2018). Solid state NMR studies of intact lipopolysaccharide endotoxin. <i>ACS Chemical Biology</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acschembio.8b00271\">https://doi.org/10.1021/acschembio.8b00271</a>"},"keyword":["Molecular Medicine","Biochemistry","General Medicine"],"date_created":"2020-09-18T10:05:09Z","month":"07","page":"2106-2113","year":"2018","publisher":"American Chemical Society","article_type":"original","status":"public","day":"02","date_published":"2018-07-02T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"}