{"date_created":"2020-08-24T06:24:03Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa":1,"oa_version":"Published Version","scopus_import":"1","publisher":"MDPI","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"10083"}]},"status":"public","quality_controlled":"1","title":"AcoMYB4, an Ananas comosus L. MYB transcription factor, functions in osmotic stress through negative regulation of ABA signaling","day":"10","file":[{"file_id":"8292","success":1,"file_size":5718755,"date_created":"2020-08-25T09:53:50Z","date_updated":"2020-08-25T09:53:50Z","content_type":"application/pdf","file_name":"2020_IntMolecSciences_Chen.pdf","access_level":"open_access","creator":"cziletti","relation":"main_file","checksum":"03b039244e6ae80580385fd9f577e2b2"}],"type":"journal_article","tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"pmid":1,"date_updated":"2024-10-29T10:22:43Z","article_processing_charge":"No","month":"08","issue":"16","date_published":"2020-08-10T00:00:00Z","publication_status":"published","abstract":[{"lang":"eng","text":"Drought and salt stress are the main environmental cues affecting the survival, development, distribution, and yield of crops worldwide. MYB transcription factors play a crucial role in plants’ biological processes, but the function of pineapple MYB genes is still obscure. In this study, one of the pineapple MYB transcription factors, AcoMYB4, was isolated and characterized. The results showed that AcoMYB4 is localized in the cell nucleus, and its expression is induced by low temperature, drought, salt stress, and hormonal stimulation, especially by abscisic acid (ABA). Overexpression of AcoMYB4 in rice and Arabidopsis enhanced plant sensitivity to osmotic stress; it led to an increase in the number stomata on leaf surfaces and lower germination rate under salt and drought stress. Furthermore, in AcoMYB4 OE lines, the membrane oxidation index, free proline, and soluble sugar contents were decreased. In contrast, electrolyte leakage and malondialdehyde (MDA) content increased significantly due to membrane injury, indicating higher sensitivity to drought and salinity stresses. Besides the above, both the expression level and activities of several antioxidant enzymes were decreased, indicating lower antioxidant activity in AcoMYB4 transgenic plants. Moreover, under osmotic stress, overexpression of AcoMYB4 inhibited ABA biosynthesis through a decrease in the transcription of genes responsible for ABA synthesis (ABA1 and ABA2) and ABA signal transduction factor ABI5. These results suggest that AcoMYB4 negatively regulates osmotic stress by attenuating cellular ABA biosynthesis and signal transduction pathways. "}],"department":[{"_id":"JiFr"}],"volume":21,"intvolume":"        21","year":"2020","file_date_updated":"2020-08-25T09:53:50Z","acknowledgement":"We would like to thank the reviewers for their helpful comments on the original manuscript. ","isi":1,"ddc":["570"],"doi":"10.3390/ijms21165727","publication":"International Journal of Molecular Sciences","author":[{"full_name":"Chen, Huihuang","last_name":"Chen","first_name":"Huihuang"},{"first_name":"Linyi","last_name":"Lai","full_name":"Lai, Linyi"},{"full_name":"Li, Lanxin","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","last_name":"Li","first_name":"Lanxin","orcid":"0000-0002-5607-272X"},{"full_name":"Liu, Liping","last_name":"Liu","first_name":"Liping"},{"first_name":"Bello Hassan","last_name":"Jakada","full_name":"Jakada, Bello Hassan"},{"first_name":"Youmei","last_name":"Huang","full_name":"Huang, Youmei"},{"full_name":"He, Qing","last_name":"He","first_name":"Qing"},{"first_name":"Mengnan","last_name":"Chai","full_name":"Chai, Mengnan"},{"last_name":"Niu","first_name":"Xiaoping","full_name":"Niu, Xiaoping"},{"full_name":"Qin, Yuan","first_name":"Yuan","last_name":"Qin"}],"has_accepted_license":"1","publication_identifier":{"issn":["16616596"],"eissn":["14220067"]},"external_id":{"pmid":["32785037"],"isi":["000565090300001"]},"citation":{"short":"H. Chen, L. Lai, L. Li, L. Liu, B.H. Jakada, Y. Huang, Q. He, M. Chai, X. Niu, Y. Qin, International Journal of Molecular Sciences 21 (2020).","ista":"Chen H, Lai L, Li L, Liu L, Jakada BH, Huang Y, He Q, Chai M, Niu X, Qin Y. 2020. AcoMYB4, an Ananas comosus L. MYB transcription factor, functions in osmotic stress through negative regulation of ABA signaling. International Journal of Molecular Sciences. 21(16), 5272.","chicago":"Chen, Huihuang, Linyi Lai, Lanxin Li, Liping Liu, Bello Hassan Jakada, Youmei Huang, Qing He, Mengnan Chai, Xiaoping Niu, and Yuan Qin. “AcoMYB4, an Ananas Comosus L. MYB Transcription Factor, Functions in Osmotic Stress through Negative Regulation of ABA Signaling.” <i>International Journal of Molecular Sciences</i>. MDPI, 2020. <a href=\"https://doi.org/10.3390/ijms21165727\">https://doi.org/10.3390/ijms21165727</a>.","apa":"Chen, H., Lai, L., Li, L., Liu, L., Jakada, B. H., Huang, Y., … Qin, Y. (2020). AcoMYB4, an Ananas comosus L. MYB transcription factor, functions in osmotic stress through negative regulation of ABA signaling. <i>International Journal of Molecular Sciences</i>. MDPI. <a href=\"https://doi.org/10.3390/ijms21165727\">https://doi.org/10.3390/ijms21165727</a>","ama":"Chen H, Lai L, Li L, et al. AcoMYB4, an Ananas comosus L. MYB transcription factor, functions in osmotic stress through negative regulation of ABA signaling. <i>International Journal of Molecular Sciences</i>. 2020;21(16). doi:<a href=\"https://doi.org/10.3390/ijms21165727\">10.3390/ijms21165727</a>","ieee":"H. Chen <i>et al.</i>, “AcoMYB4, an Ananas comosus L. MYB transcription factor, functions in osmotic stress through negative regulation of ABA signaling,” <i>International Journal of Molecular Sciences</i>, vol. 21, no. 16. MDPI, 2020.","mla":"Chen, Huihuang, et al. “AcoMYB4, an Ananas Comosus L. MYB Transcription Factor, Functions in Osmotic Stress through Negative Regulation of ABA Signaling.” <i>International Journal of Molecular Sciences</i>, vol. 21, no. 16, 5272, MDPI, 2020, doi:<a href=\"https://doi.org/10.3390/ijms21165727\">10.3390/ijms21165727</a>."},"article_type":"original","language":[{"iso":"eng"}],"_id":"8283","article_number":"5272"}