{"publication":"International Journal of Molecular Sciences","publication_status":"published","article_number":"5693","abstract":[{"lang":"eng","text":"Birch pollen allergy is among the most prevalent pollen allergies in Northern and Central Europe. This IgE-mediated disease can be treated with allergen immunotherapy (AIT), which typically gives rise to IgG antibodies inducing tolerance. Although the main mechanisms of allergen immunotherapy (AIT) are known, questions regarding possible Fc-mediated effects of IgG antibodies remain unanswered. This can mainly be attributed to the unavailability of appropriate tools, i.e., well-characterised recombinant antibodies (rAbs). We hereby aimed at providing human rAbs of several classes for mechanistic studies and as possible candidates for passive immunotherapy. We engineered IgE, IgG1, and IgG4 sharing the same variable region against the major birch pollen allergen Bet v 1 using Polymerase Incomplete Primer Extension (PIPE) cloning. We tested IgE functionality and IgG blocking capabilities using appropriate model cell lines. In vitro studies showed IgE engagement with FcεRI and CD23 and Bet v 1-dependent degranulation. Overall, we hereby present fully functional, human IgE, IgG1, and IgG4 sharing the same variable region against Bet v 1 and showcase possible applications in first mechanistic studies. Furthermore, our IgG antibodies might be useful candidates for passive immunotherapy of birch pollen allergy."}],"author":[{"full_name":"Köhler, Verena K.","first_name":"Verena K.","orcid":"0000-0001-5581-398X","last_name":"Köhler"},{"last_name":"Crescioli","first_name":"Silvia","full_name":"Crescioli, Silvia","orcid":"0000-0002-1909-5957"},{"last_name":"Fazekas-Singer","id":"36432834-F248-11E8-B48F-1D18A9856A87","full_name":"Fazekas-Singer, Judit","first_name":"Judit","orcid":"0000-0002-8777-3502"},{"full_name":"Bax, Heather J.","first_name":"Heather J.","orcid":"0000-0003-0432-4160","last_name":"Bax"},{"last_name":"Hofer","first_name":"Gerhard","full_name":"Hofer, Gerhard"},{"full_name":"Pranger, Christina L.","first_name":"Christina L.","last_name":"Pranger"},{"last_name":"Hufnagl","first_name":"Karin","full_name":"Hufnagl, Karin"},{"last_name":"Bianchini","orcid":"0000-0003-0351-6937","full_name":"Bianchini, Rodolfo","first_name":"Rodolfo"},{"last_name":"Flicker","orcid":"0000-0003-4768-8693","full_name":"Flicker, Sabine","first_name":"Sabine"},{"last_name":"Keller","full_name":"Keller, Walter","first_name":"Walter","orcid":"0000-0002-2261-958X"},{"last_name":"Karagiannis","orcid":"0000-0002-4100-7810","full_name":"Karagiannis, Sophia N.","first_name":"Sophia N."},{"full_name":"Jensen-Jarolim, Erika","first_name":"Erika","orcid":"0000-0003-4019-5765","last_name":"Jensen-Jarolim"}],"doi":"10.3390/ijms21165693","_id":"8225","publication_identifier":{"issn":["1422-0067"]},"volume":21,"date_updated":"2021-01-12T08:17:34Z","type":"journal_article","has_accepted_license":"1","extern":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ddc":["570"],"file_date_updated":"2020-09-10T07:06:22Z","oa":1,"status":"public","external_id":{"pmid":["32784509"]},"citation":{"apa":"Köhler, V. K., Crescioli, S., Singer, J., Bax, H. J., Hofer, G., Pranger, C. L., … Jensen-Jarolim, E. (2020). Filling the antibody pipeline in allergy: PIPE cloning of IgE, IgG1 and IgG4 against the major birch pollen allergen Bet v 1. <i>International Journal of Molecular Sciences</i>. MDPI. <a href=\"https://doi.org/10.3390/ijms21165693\">https://doi.org/10.3390/ijms21165693</a>","ista":"Köhler VK, Crescioli S, Singer J, Bax HJ, Hofer G, Pranger CL, Hufnagl K, Bianchini R, Flicker S, Keller W, Karagiannis SN, Jensen-Jarolim E. 2020. Filling the antibody pipeline in allergy: PIPE cloning of IgE, IgG1 and IgG4 against the major birch pollen allergen Bet v 1. International Journal of Molecular Sciences. 21(16), 5693.","chicago":"Köhler, Verena K., Silvia Crescioli, Judit Singer, Heather J. Bax, Gerhard Hofer, Christina L. Pranger, Karin Hufnagl, et al. “Filling the Antibody Pipeline in Allergy: PIPE Cloning of IgE, IgG1 and IgG4 against the Major Birch Pollen Allergen Bet v 1.” <i>International Journal of Molecular Sciences</i>. MDPI, 2020. <a href=\"https://doi.org/10.3390/ijms21165693\">https://doi.org/10.3390/ijms21165693</a>.","ieee":"V. K. Köhler <i>et al.</i>, “Filling the antibody pipeline in allergy: PIPE cloning of IgE, IgG1 and IgG4 against the major birch pollen allergen Bet v 1,” <i>International Journal of Molecular Sciences</i>, vol. 21, no. 16. MDPI, 2020.","mla":"Köhler, Verena K., et al. “Filling the Antibody Pipeline in Allergy: PIPE Cloning of IgE, IgG1 and IgG4 against the Major Birch Pollen Allergen Bet v 1.” <i>International Journal of Molecular Sciences</i>, vol. 21, no. 16, 5693, MDPI, 2020, doi:<a href=\"https://doi.org/10.3390/ijms21165693\">10.3390/ijms21165693</a>.","ama":"Köhler VK, Crescioli S, Singer J, et al. Filling the antibody pipeline in allergy: PIPE cloning of IgE, IgG1 and IgG4 against the major birch pollen allergen Bet v 1. <i>International Journal of Molecular Sciences</i>. 2020;21(16). doi:<a href=\"https://doi.org/10.3390/ijms21165693\">10.3390/ijms21165693</a>","short":"V.K. Köhler, S. Crescioli, J. Singer, H.J. Bax, G. Hofer, C.L. Pranger, K. Hufnagl, R. Bianchini, S. Flicker, W. Keller, S.N. Karagiannis, E. Jensen-Jarolim, International Journal of Molecular Sciences 21 (2020)."},"date_created":"2020-08-10T11:47:29Z","month":"08","article_processing_charge":"No","issue":"16","oa_version":"Published Version","title":"Filling the antibody pipeline in allergy: PIPE cloning of IgE, IgG1 and IgG4 against the major birch pollen allergen Bet v 1","quality_controlled":"1","intvolume":"        21","file":[{"file_size":2680908,"content_type":"application/pdf","date_updated":"2020-09-10T07:06:22Z","creator":"dernst","file_id":"8356","success":1,"relation":"main_file","file_name":"2020_IntMolecSciences_Koehler.pdf","access_level":"open_access","checksum":"dac7ccef7cdcea9be292664d8c488425","date_created":"2020-09-10T07:06:22Z"}],"language":[{"iso":"eng"}],"date_published":"2020-08-08T00:00:00Z","publisher":"MDPI","year":"2020","article_type":"original","day":"08","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"pmid":1}