{"publication_status":"published","issue":"7535","publication":"Nature","abstract":[{"text":"Human immunodeficiency virus type 1 (HIV-1) assembly proceeds in two stages. First, the 55 kilodalton viral Gag polyprotein assembles into a hexameric protein lattice at the plasma membrane of the infected cell, inducing budding and release of an immature particle. Second, Gag is cleaved by the viral protease, leading to internal rearrangement of the virus into the mature, infectious form. Immature and mature HIV-1 particles are heterogeneous in size and morphology, preventing high-resolution analysis of their protein arrangement in situ by conventional structural biology methods. Here we apply cryo-electron tomography and sub-tomogram averaging methods to resolve the structure of the capsid lattice within intact immature HIV-1 particles at subnanometre resolution, allowing unambiguous positioning of all α-helices. The resulting model reveals tertiary and quaternary structural interactions that mediate HIV-1 assembly. Strikingly, these interactions differ from those predicted by the current model based on in vitro-assembled arrays of Gag-derived proteins from Mason-Pfizer monkey virus. To validate this difference, we solve the structure of the capsid lattice within intact immature Mason-Pfizer monkey virus particles. Comparison with the immature HIV-1 structure reveals that retroviral capsid proteins, while having conserved tertiary structures, adopt different quaternary arrangements during virus assembly. The approach demonstrated here should be applicable to determine structures of other proteins at subnanometre resolution within heterogeneous environments.","lang":"eng"}],"doi":"10.1038/nature13838","author":[{"full_name":"Florian Schur","first_name":"Florian","orcid":"0000-0003-4790-8078","last_name":"Schur","id":"48AD8942-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Hagen","full_name":"Hagen, Wim J","first_name":"Wim"},{"last_name":"Rumlová","full_name":"Rumlová, Michaela","first_name":"Michaela"},{"last_name":"Ruml","full_name":"Ruml, Tomáš","first_name":"Tomáš"},{"full_name":"Müller B","first_name":"B","last_name":"Müller"},{"first_name":"Hans","full_name":"Kraüsslich, Hans Georg","last_name":"Kraüsslich"},{"first_name":"John","full_name":"Briggs, John A","last_name":"Briggs"}],"_id":"814","title":"Structure of the immature HIV-1 capsid in intact virus particles at 8.8 Å resolution","quality_controlled":0,"intvolume":"       517","volume":517,"publist_id":"6836","date_updated":"2021-01-12T08:17:08Z","type":"journal_article","extern":1,"date_published":"2015-01-22T00:00:00Z","page":"505 - 508","year":"2015","publisher":"Nature Publishing Group","status":"public","day":"22","citation":{"ama":"Schur FK, Hagen W, Rumlová M, et al. Structure of the immature HIV-1 capsid in intact virus particles at 8.8 Å resolution. <i>Nature</i>. 2015;517(7535):505-508. doi:<a href=\"https://doi.org/10.1038/nature13838\">10.1038/nature13838</a>","mla":"Schur, Florian KM, et al. “Structure of the Immature HIV-1 Capsid in Intact Virus Particles at 8.8 Å Resolution.” <i>Nature</i>, vol. 517, no. 7535, Nature Publishing Group, 2015, pp. 505–08, doi:<a href=\"https://doi.org/10.1038/nature13838\">10.1038/nature13838</a>.","short":"F.K. Schur, W. Hagen, M. Rumlová, T. Ruml, B. Müller, H. Kraüsslich, J. Briggs, Nature 517 (2015) 505–508.","ieee":"F. K. Schur <i>et al.</i>, “Structure of the immature HIV-1 capsid in intact virus particles at 8.8 Å resolution,” <i>Nature</i>, vol. 517, no. 7535. Nature Publishing Group, pp. 505–508, 2015.","chicago":"Schur, Florian KM, Wim Hagen, Michaela Rumlová, Tomáš Ruml, B Müller, Hans Kraüsslich, and John Briggs. “Structure of the Immature HIV-1 Capsid in Intact Virus Particles at 8.8 Å Resolution.” <i>Nature</i>. Nature Publishing Group, 2015. <a href=\"https://doi.org/10.1038/nature13838\">https://doi.org/10.1038/nature13838</a>.","ista":"Schur FK, Hagen W, Rumlová M, Ruml T, Müller B, Kraüsslich H, Briggs J. 2015. Structure of the immature HIV-1 capsid in intact virus particles at 8.8 Å resolution. Nature. 517(7535), 505–508.","apa":"Schur, F. K., Hagen, W., Rumlová, M., Ruml, T., Müller, B., Kraüsslich, H., &#38; Briggs, J. (2015). Structure of the immature HIV-1 capsid in intact virus particles at 8.8 Å resolution. <i>Nature</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nature13838\">https://doi.org/10.1038/nature13838</a>"},"date_created":"2018-12-11T11:48:39Z","month":"01","acknowledgement":"This study was supported by Deutsche Forschungsgemeinschaft grants BR 3635/2-1 to J.A.G.B., KR 906/7-1 to H.-G.K. and by Grant Agency of the Czech Republic 14-15326S to M.R. The Briggs laboratory acknowledges financial support from the European Molecular Biology Laboratory and from the Chica und Heinz Schaller Stiftung. We thank B. Glass, M. Anders and S. Mattei for preparation of samples, and R. Hadravova, K. H. Bui, F. Thommen, M. Schorb, S. Dodonova, S. Glatt, P. Ulbrich and T. Bharat for technical support and/or discussion. This study was technically supported by the European Molecular Biology Laboratory IT services unit."}