{"day":"16","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"article_type":"original","year":"2020","license":"https://creativecommons.org/licenses/by/4.0/","date_published":"2020-12-16T00:00:00Z","title":"Self-assembly-based posttranslational protein oscillators","quality_controlled":"1","oa_version":"Published Version","issue":"51","language":[{"iso":"eng"}],"intvolume":"         6","file":[{"content_type":"application/pdf","file_size":1259758,"access_level":"open_access","success":1,"relation":"main_file","file_name":"2020_ScienceAdv_Kimchi.pdf","date_created":"2021-04-12T08:33:23Z","checksum":"eb6d950b6a68ddc4a2fb31ec80a2a1bd","file_id":"9320","creator":"dernst","date_updated":"2021-04-12T08:33:23Z"}],"status":"public","file_date_updated":"2021-04-12T08:33:23Z","oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ddc":["570"],"article_processing_charge":"No","date_created":"2020-04-30T12:07:55Z","month":"12","citation":{"ieee":"O. Kimchi <i>et al.</i>, “Self-assembly-based posttranslational protein oscillators,” <i>Science Advances</i>, vol. 6, no. 51. 2020.","mla":"Kimchi, Ofer, et al. “Self-Assembly-Based Posttranslational Protein Oscillators.” <i>Science Advances</i>, vol. 6, no. 51, eabc1939, 2020, doi:<a href=\"https://doi.org/10.1126/sciadv.abc1939\">10.1126/sciadv.abc1939</a>.","ama":"Kimchi O, Goodrich CP, Courbet A, et al. Self-assembly-based posttranslational protein oscillators. <i>Science Advances</i>. 2020;6(51). doi:<a href=\"https://doi.org/10.1126/sciadv.abc1939\">10.1126/sciadv.abc1939</a>","short":"O. Kimchi, C.P. Goodrich, A. Courbet, A.I. Curatolo, N.B. Woodall, D. Baker, M.P. Brenner, Science Advances 6 (2020).","apa":"Kimchi, O., Goodrich, C. P., Courbet, A., Curatolo, A. I., Woodall, N. B., Baker, D., &#38; Brenner, M. P. (2020). Self-assembly-based posttranslational protein oscillators. <i>Science Advances</i>. <a href=\"https://doi.org/10.1126/sciadv.abc1939\">https://doi.org/10.1126/sciadv.abc1939</a>","chicago":"Kimchi, Ofer, Carl Peter Goodrich, Alexis Courbet, Agnese I. Curatolo, Nicholas B. Woodall, David Baker, and Michael P. Brenner. “Self-Assembly-Based Posttranslational Protein Oscillators.” <i>Science Advances</i>, 2020. <a href=\"https://doi.org/10.1126/sciadv.abc1939\">https://doi.org/10.1126/sciadv.abc1939</a>.","ista":"Kimchi O, Goodrich CP, Courbet A, Curatolo AI, Woodall NB, Baker D, Brenner MP. 2020. Self-assembly-based posttranslational protein oscillators. Science Advances. 6(51), eabc1939."},"doi":"10.1126/sciadv.abc1939","author":[{"first_name":"Ofer","full_name":"Kimchi, Ofer","last_name":"Kimchi"},{"last_name":"Goodrich","id":"EB352CD2-F68A-11E9-89C5-A432E6697425","full_name":"Goodrich, Carl Peter","first_name":"Carl Peter","orcid":"0000-0002-1307-5074"},{"last_name":"Courbet","first_name":"Alexis","full_name":"Courbet, Alexis"},{"last_name":"Curatolo","full_name":"Curatolo, Agnese I.","first_name":"Agnese I."},{"full_name":"Woodall, Nicholas B.","first_name":"Nicholas B.","last_name":"Woodall"},{"last_name":"Baker","first_name":"David","full_name":"Baker, David"},{"full_name":"Brenner, Michael P.","first_name":"Michael P.","last_name":"Brenner"}],"_id":"7778","abstract":[{"lang":"eng","text":"Recent advances in synthetic posttranslational protein circuits are substantially impacting the landscape of cellular engineering and offer several advantages compared to traditional gene circuits. However, engineering dynamic phenomena such as oscillations in protein-level circuits remains an outstanding challenge. Few examples of biological posttranslational oscillators are known, necessitating theoretical progress to determine realizable oscillators. We construct mathematical models for two posttranslational oscillators, using few components that interact only through reversible binding and phosphorylation/dephosphorylation reactions. Our designed oscillators rely on the self-assembly of two protein species into multimeric functional enzymes that respectively inhibit and enhance this self-assembly. We limit our analysis to within experimental constraints, finding (i) significant portions of the restricted parameter space yielding oscillations and (ii) that oscillation periods can be tuned by several orders of magnitude using recent advances in computational protein design. Our work paves the way for the rational design and realization of protein-based dynamic systems."}],"article_number":"eabc1939","publication_status":"published","publication":"Science Advances","type":"journal_article","has_accepted_license":"1","extern":"1","volume":6,"date_updated":"2021-04-12T08:35:19Z"}