{"status":"public","title":"Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis","quality_controlled":"1","publisher":"Oxford University Press","date_created":"2020-04-06T10:57:08Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa_version":"Published Version","oa":1,"abstract":[{"lang":"eng","text":"In plant cells, environmental stressors promote changes in connectivity between the cortical ER and the PM. Although this process is tightly regulated in space and time, the molecular signals and structural components mediating these changes in inter-organelle communication are only starting to be characterized. In this report, we confirm the presence of a putative tethering complex containing the synaptotagmins 1 and 5 (SYT1 and SYT5) and the Ca2+ and lipid binding protein 1 (CLB1/SYT7). This complex is enriched at ER-PM contact sites (EPCS), have slow responses to changes in extracellular Ca2+, and display severe cytoskeleton-dependent rearrangements in response to the trivalent lanthanum (La3+) and gadolinium (Gd3+) rare earth elements (REEs). Although REEs are generally used as non-selective cation channel blockers at the PM, here we show that the slow internalization of REEs into the cytosol underlies the activation of the Ca2+/Calmodulin intracellular signaling, the accumulation of phosphatidylinositol-4-phosphate (PI4P) at the PM, and the cytoskeleton-dependent rearrangement of the SYT1/SYT5 EPCS complexes. We propose that the observed EPCS rearrangements act as a slow adaptive response to sustained stress conditions, and that this process involves the accumulation of stress-specific phosphoinositides species at the PM."}],"department":[{"_id":"JiFr"}],"issue":"14","publication_status":"published","date_published":"2020-07-06T00:00:00Z","pmid":1,"date_updated":"2023-08-18T10:27:52Z","tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"month":"07","article_processing_charge":"No","day":"06","type":"journal_article","file":[{"date_updated":"2020-10-06T07:41:35Z","date_created":"2020-10-06T07:41:35Z","file_size":1916031,"file_id":"8613","success":1,"relation":"main_file","checksum":"b06aaaa93dc41896da805fe4b75cf3a1","file_name":"2020_JourExperimBotany_Lee.pdf","creator":"dernst","access_level":"open_access","content_type":"application/pdf"}],"ddc":["580"],"isi":1,"file_date_updated":"2020-10-06T07:41:35Z","year":"2020","volume":71,"intvolume":"        71","_id":"7646","language":[{"iso":"eng"}],"article_type":"original","citation":{"mla":"Lee, E., et al. “Rare Earth Elements Induce Cytoskeleton-Dependent and PI4P-Associated Rearrangement of SYT1/SYT5 ER-PM Contact Site Complexes in Arabidopsis.” <i>Journal of Experimental Botany</i>, vol. 71, no. 14, Oxford University Press, 2020, pp. 3986–3998, doi:<a href=\"https://doi.org/10.1093/jxb/eraa138\">10.1093/jxb/eraa138</a>.","ieee":"E. Lee <i>et al.</i>, “Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis,” <i>Journal of Experimental Botany</i>, vol. 71, no. 14. Oxford University Press, pp. 3986–3998, 2020.","apa":"Lee, E., Vila Nova Santana, B., Samuels, E., Benitez-Fuente, F., Corsi, E., Botella, M., … Rosado, A. (2020). Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis. <i>Journal of Experimental Botany</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/jxb/eraa138\">https://doi.org/10.1093/jxb/eraa138</a>","chicago":"Lee, E, B Vila Nova Santana, E Samuels, F Benitez-Fuente, E Corsi, MA Botella, J Perez-Sancho, et al. “Rare Earth Elements Induce Cytoskeleton-Dependent and PI4P-Associated Rearrangement of SYT1/SYT5 ER-PM Contact Site Complexes in Arabidopsis.” <i>Journal of Experimental Botany</i>. Oxford University Press, 2020. <a href=\"https://doi.org/10.1093/jxb/eraa138\">https://doi.org/10.1093/jxb/eraa138</a>.","ista":"Lee E, Vila Nova Santana B, Samuels E, Benitez-Fuente F, Corsi E, Botella M, Perez-Sancho J, Vanneste S, Friml J, Macho A, Alves Azevedo A, Rosado A. 2020. Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis. Journal of Experimental Botany. 71(14), 3986–3998.","ama":"Lee E, Vila Nova Santana B, Samuels E, et al. Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis. <i>Journal of Experimental Botany</i>. 2020;71(14):3986–3998. doi:<a href=\"https://doi.org/10.1093/jxb/eraa138\">10.1093/jxb/eraa138</a>","short":"E. Lee, B. Vila Nova Santana, E. Samuels, F. Benitez-Fuente, E. Corsi, M. Botella, J. Perez-Sancho, S. Vanneste, J. Friml, A. Macho, A. Alves Azevedo, A. Rosado, Journal of Experimental Botany 71 (2020) 3986–3998."},"publication_identifier":{"eissn":["1460-2431"],"issn":["0022-0957"]},"external_id":{"isi":["000553125400007"],"pmid":["32179893"]},"publication":"Journal of Experimental Botany","author":[{"last_name":"Lee","first_name":"E","full_name":"Lee, E"},{"full_name":"Vila Nova Santana, B","first_name":"B","last_name":"Vila Nova Santana"},{"first_name":"E","last_name":"Samuels","full_name":"Samuels, E"},{"full_name":"Benitez-Fuente, F","last_name":"Benitez-Fuente","first_name":"F"},{"last_name":"Corsi","first_name":"E","full_name":"Corsi, E"},{"last_name":"Botella","first_name":"MA","full_name":"Botella, MA"},{"full_name":"Perez-Sancho, J","last_name":"Perez-Sancho","first_name":"J"},{"last_name":"Vanneste","first_name":"S","full_name":"Vanneste, S"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jiří"},{"first_name":"A","last_name":"Macho","full_name":"Macho, A"},{"full_name":"Alves Azevedo, A","first_name":"A","last_name":"Alves Azevedo"},{"last_name":"Rosado","first_name":"A","full_name":"Rosado, A"}],"has_accepted_license":"1","doi":"10.1093/jxb/eraa138","page":"3986–3998"}