{"intvolume":"        10","oa_version":"Published Version","file_date_updated":"2020-07-14T12:47:31Z","file":[{"relation":"main_file","access_level":"open_access","content_type":"application/pdf","date_created":"2019-05-20T07:33:54Z","file_name":"2019_NatureComm_Chassin.pdf","file_size":1191827,"checksum":"e214d3e4f8c81e35981583c4569b51b8","date_updated":"2020-07-14T12:47:31Z","creator":"dernst","file_id":"6471"}],"_id":"6465","abstract":[{"lang":"eng","text":"Tight control over protein degradation is a fundamental requirement for cells to respond rapidly to various stimuli and adapt to a fluctuating environment. Here we develop a versatile, easy-to-handle library of destabilizing tags (degrons) for the precise regulation of protein expression profiles in mammalian cells by modulating target protein half-lives in a predictable manner. Using the well-established tetracycline gene-regulation system as a model, we show that the dynamics of protein expression can be tuned by fusing appropriate degron tags to gene regulators. Next, we apply this degron library to tune a synthetic pulse-generating circuit in mammalian cells. With this toolbox we establish a set of pulse generators with tailored pulse lengths and magnitudes of protein expression. This methodology will prove useful in the functional roles of essential proteins, fine-tuning of gene-expression systems, and enabling a higher complexity in the design of synthetic biological systems in mammalian cells."}],"month":"05","external_id":{"isi":["000466338600006"]},"article_number":"2013","title":"A modular degron library for synthetic circuits in mammalian cells","date_published":"2019-05-01T00:00:00Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_updated":"2023-08-25T10:33:51Z","day":"01","date_created":"2019-05-19T21:59:14Z","language":[{"iso":"eng"}],"doi":"10.1038/s41467-019-09974-5","status":"public","isi":1,"issue":"1","type":"journal_article","department":[{"_id":"CaGu"}],"publisher":"Springer Nature","citation":{"chicago":"Chassin, Hélène, Marius Müller, Marcel Tigges, Leo Scheller, Moritz Lang, and Martin Fussenegger. “A Modular Degron Library for Synthetic Circuits in Mammalian Cells.” <i>Nature Communications</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41467-019-09974-5\">https://doi.org/10.1038/s41467-019-09974-5</a>.","apa":"Chassin, H., Müller, M., Tigges, M., Scheller, L., Lang, M., &#38; Fussenegger, M. (2019). A modular degron library for synthetic circuits in mammalian cells. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-019-09974-5\">https://doi.org/10.1038/s41467-019-09974-5</a>","mla":"Chassin, Hélène, et al. “A Modular Degron Library for Synthetic Circuits in Mammalian Cells.” <i>Nature Communications</i>, vol. 10, no. 1, 2013, Springer Nature, 2019, doi:<a href=\"https://doi.org/10.1038/s41467-019-09974-5\">10.1038/s41467-019-09974-5</a>.","short":"H. Chassin, M. Müller, M. Tigges, L. Scheller, M. Lang, M. Fussenegger, Nature Communications 10 (2019).","ista":"Chassin H, Müller M, Tigges M, Scheller L, Lang M, Fussenegger M. 2019. A modular degron library for synthetic circuits in mammalian cells. Nature Communications. 10(1), 2013.","ama":"Chassin H, Müller M, Tigges M, Scheller L, Lang M, Fussenegger M. A modular degron library for synthetic circuits in mammalian cells. <i>Nature Communications</i>. 2019;10(1). doi:<a href=\"https://doi.org/10.1038/s41467-019-09974-5\">10.1038/s41467-019-09974-5</a>","ieee":"H. Chassin, M. Müller, M. Tigges, L. Scheller, M. Lang, and M. Fussenegger, “A modular degron library for synthetic circuits in mammalian cells,” <i>Nature Communications</i>, vol. 10, no. 1. Springer Nature, 2019."},"author":[{"full_name":"Chassin, Hélène","last_name":"Chassin","first_name":"Hélène"},{"full_name":"Müller, Marius","last_name":"Müller","first_name":"Marius"},{"last_name":"Tigges","first_name":"Marcel","full_name":"Tigges, Marcel"},{"full_name":"Scheller, Leo","first_name":"Leo","last_name":"Scheller"},{"full_name":"Lang, Moritz","first_name":"Moritz","id":"29E0800A-F248-11E8-B48F-1D18A9856A87","last_name":"Lang"},{"first_name":"Martin","last_name":"Fussenegger","full_name":"Fussenegger, Martin"}],"related_material":{"link":[{"relation":"erratum","url":"https://doi.org/10.1038/s41467-023-36111-0"}]},"scopus_import":"1","publication_status":"published","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"year":"2019","quality_controlled":"1","ddc":["570"],"volume":10,"article_processing_charge":"No","has_accepted_license":"1","oa":1,"publication_identifier":{"eissn":["20411723"]},"publication":"Nature Communications"}