{"publisher":"ASPB","scopus_import":"1","oa":1,"oa_version":"Published Version","date_created":"2019-04-09T08:38:20Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","quality_controlled":"1","title":"Pinstatic acid promotes auxin transport by inhibiting PIN internalization","related_material":{"record":[{"status":"public","id":"11626","relation":"dissertation_contains"},{"status":"public","id":"8822","relation":"dissertation_contains"}]},"status":"public","article_processing_charge":"No","month":"06","date_updated":"2024-03-25T23:30:21Z","pmid":1,"type":"journal_article","day":"01","department":[{"_id":"JiFr"}],"abstract":[{"lang":"eng","text":"Polar auxin transport plays a pivotal role in plant growth and development. PIN auxin efflux carriers regulate directional auxin movement by establishing local auxin maxima, minima, and gradients that drive multiple developmental processes and responses to environmental signals. Auxin has been proposed to modulate its own transport by regulating subcellular PIN trafficking via processes such as clathrin-mediated PIN endocytosis and constitutive recycling. Here, we further investigated the mechanisms by which auxin affects PIN trafficking by screening auxin analogs and identified pinstatic acid (PISA) as a positive modulator of polar auxin transport in Arabidopsis thaliana. PISA had an auxin-like effect on hypocotyl elongation and adventitious root formation via positive regulation of auxin transport. PISA did not activate SCFTIR1/AFB signaling and yet induced PIN accumulation at the cell surface by inhibiting PIN internalization from the plasma membrane. This work demonstrates PISA to be a promising chemical tool to dissect the regulatory mechanisms behind subcellular PIN trafficking and auxin transport."}],"main_file_link":[{"url":"https://doi.org/10.1104/pp.19.00201","open_access":"1"}],"publication_status":"published","date_published":"2019-06-01T00:00:00Z","issue":"2","year":"2019","intvolume":"       180","volume":180,"project":[{"call_identifier":"H2020","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"}],"isi":1,"acknowledgement":"We thank Dr. H. Fukaki (University of Kobe), Dr. R. Offringa (Leiden University), Dr. Jianwei Pan (Zhejiang Normal University), and Dr. M. Estelle (University of California at San Diego) for providing mutants and transgenic line seeds.\r\nThis work was supported by the Ministry of Education, Culture, Sports, Science, and Technology (Grant-in-Aid for Scientific Research no. JP25114518 to K.H.), the Biotechnology and Biological Sciences Research Council (award no. BB/L009366/1 to R.N. and S.K.), and the European Union’s Horizon2020 program (European Research Council grant agreement no. 742985 to J.F.).","external_id":{"isi":["000470086100045"],"pmid":["30936248"]},"publication_identifier":{"issn":["0032-0889"],"eissn":["1532-2548"]},"doi":"10.1104/pp.19.00201","page":"1152-1165","publication":"Plant Physiology","author":[{"full_name":"Oochi, A","first_name":"A","last_name":"Oochi"},{"orcid":"0000-0003-2140-7195","id":"4800CC20-F248-11E8-B48F-1D18A9856A87","full_name":"Hajny, Jakub","last_name":"Hajny","first_name":"Jakub"},{"last_name":"Fukui","first_name":"K","full_name":"Fukui, K"},{"full_name":"Nakao, Y","last_name":"Nakao","first_name":"Y"},{"orcid":"0000-0003-1286-7368","last_name":"Gallei","first_name":"Michelle C","full_name":"Gallei, Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Quareshy","first_name":"M","full_name":"Quareshy, M"},{"full_name":"Takahashi, K","last_name":"Takahashi","first_name":"K"},{"first_name":"T","last_name":"Kinoshita","full_name":"Kinoshita, T"},{"last_name":"Harborough","first_name":"SR","full_name":"Harborough, SR"},{"first_name":"S","last_name":"Kepinski","full_name":"Kepinski, S"},{"full_name":"Kasahara, H","last_name":"Kasahara","first_name":"H"},{"full_name":"Napier, RM","last_name":"Napier","first_name":"RM"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří","orcid":"0000-0002-8302-7596"},{"full_name":"Hayashi, KI","first_name":"KI","last_name":"Hayashi"}],"language":[{"iso":"eng"}],"_id":"6260","ec_funded":1,"article_type":"original","citation":{"mla":"Oochi, A., et al. “Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN Internalization.” <i>Plant Physiology</i>, vol. 180, no. 2, ASPB, 2019, pp. 1152–65, doi:<a href=\"https://doi.org/10.1104/pp.19.00201\">10.1104/pp.19.00201</a>.","ieee":"A. Oochi <i>et al.</i>, “Pinstatic acid promotes auxin transport by inhibiting PIN internalization,” <i>Plant Physiology</i>, vol. 180, no. 2. ASPB, pp. 1152–1165, 2019.","ama":"Oochi A, Hajny J, Fukui K, et al. Pinstatic acid promotes auxin transport by inhibiting PIN internalization. <i>Plant Physiology</i>. 2019;180(2):1152-1165. doi:<a href=\"https://doi.org/10.1104/pp.19.00201\">10.1104/pp.19.00201</a>","ista":"Oochi A, Hajny J, Fukui K, Nakao Y, Gallei MC, Quareshy M, Takahashi K, Kinoshita T, Harborough S, Kepinski S, Kasahara H, Napier R, Friml J, Hayashi K. 2019. Pinstatic acid promotes auxin transport by inhibiting PIN internalization. Plant Physiology. 180(2), 1152–1165.","apa":"Oochi, A., Hajny, J., Fukui, K., Nakao, Y., Gallei, M. C., Quareshy, M., … Hayashi, K. (2019). Pinstatic acid promotes auxin transport by inhibiting PIN internalization. <i>Plant Physiology</i>. ASPB. <a href=\"https://doi.org/10.1104/pp.19.00201\">https://doi.org/10.1104/pp.19.00201</a>","chicago":"Oochi, A, Jakub Hajny, K Fukui, Y Nakao, Michelle C Gallei, M Quareshy, K Takahashi, et al. “Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN Internalization.” <i>Plant Physiology</i>. ASPB, 2019. <a href=\"https://doi.org/10.1104/pp.19.00201\">https://doi.org/10.1104/pp.19.00201</a>.","short":"A. Oochi, J. Hajny, K. Fukui, Y. Nakao, M.C. Gallei, M. Quareshy, K. Takahashi, T. Kinoshita, S. Harborough, S. Kepinski, H. Kasahara, R. Napier, J. Friml, K. Hayashi, Plant Physiology 180 (2019) 1152–1165."}}