{"_id":"600","doi":"10.1016/j.cell.2017.03.003","author":[{"last_name":"Engel","first_name":"Christoph","full_name":"Engel, Christoph"},{"last_name":"Gubbey","first_name":"Tobias","full_name":"Gubbey, Tobias"},{"full_name":"Neyer, Simon","first_name":"Simon","last_name":"Neyer"},{"first_name":"Sarah","full_name":"Sainsbury, Sarah","last_name":"Sainsbury"},{"first_name":"Christiane","full_name":"Oberthuer, Christiane","last_name":"Oberthuer"},{"first_name":"Carlo","full_name":"Baejen, Carlo","last_name":"Baejen"},{"last_name":"Bernecky","id":"2CB9DFE2-F248-11E8-B48F-1D18A9856A87","full_name":"Bernecky, Carrie A","first_name":"Carrie A","orcid":"0000-0003-0893-7036"},{"last_name":"Cramer","full_name":"Cramer, Patrick","first_name":"Patrick"}],"quality_controlled":"1","title":"Structural basis of RNA polymerase I transcription initiation","issue":"1","publication_status":"published","publication":"Cell","oa_version":"None","abstract":[{"lang":"eng","text":"Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron microscopy (cryo-EM) to obtain a molecular model for basal Pol I initiation. The three-subunit CF binds upstream promoter DNA, docks to the Pol I-Rrn3 complex, and loads DNA into the expanded active center cleft of the polymerase. DNA unwinding between the Pol I protrusion and clamp domains enables cleft contraction, resulting in an active Pol I conformation and RNA synthesis. Comparison with the Pol II system suggests that promoter specificity relies on a distinct “bendability” and “meltability” of the promoter sequence that enables contacts between initiation factors, DNA, and polymerase."}],"date_updated":"2021-01-12T08:05:36Z","publist_id":"7204","volume":169,"extern":"1","type":"journal_article","publication_identifier":{"issn":["00928674"]},"intvolume":"       169","language":[{"iso":"eng"}],"publisher":"Cell Press","year":"2017","status":"public","page":"120 - 131.e22","day":"23","date_published":"2017-03-23T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"No","citation":{"apa":"Engel, C., Gubbey, T., Neyer, S., Sainsbury, S., Oberthuer, C., Baejen, C., … Cramer, P. (2017). Structural basis of RNA polymerase I transcription initiation. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2017.03.003\">https://doi.org/10.1016/j.cell.2017.03.003</a>","chicago":"Engel, Christoph, Tobias Gubbey, Simon Neyer, Sarah Sainsbury, Christiane Oberthuer, Carlo Baejen, Carrie Bernecky, and Patrick Cramer. “Structural Basis of RNA Polymerase I Transcription Initiation.” <i>Cell</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.cell.2017.03.003\">https://doi.org/10.1016/j.cell.2017.03.003</a>.","ista":"Engel C, Gubbey T, Neyer S, Sainsbury S, Oberthuer C, Baejen C, Bernecky C, Cramer P. 2017. Structural basis of RNA polymerase I transcription initiation. Cell. 169(1), 120–131.e22.","ieee":"C. Engel <i>et al.</i>, “Structural basis of RNA polymerase I transcription initiation,” <i>Cell</i>, vol. 169, no. 1. Cell Press, p. 120–131.e22, 2017.","short":"C. Engel, T. Gubbey, S. Neyer, S. Sainsbury, C. Oberthuer, C. Baejen, C. Bernecky, P. Cramer, Cell 169 (2017) 120–131.e22.","ama":"Engel C, Gubbey T, Neyer S, et al. Structural basis of RNA polymerase I transcription initiation. <i>Cell</i>. 2017;169(1):120-131.e22. doi:<a href=\"https://doi.org/10.1016/j.cell.2017.03.003\">10.1016/j.cell.2017.03.003</a>","mla":"Engel, Christoph, et al. “Structural Basis of RNA Polymerase I Transcription Initiation.” <i>Cell</i>, vol. 169, no. 1, Cell Press, 2017, p. 120–131.e22, doi:<a href=\"https://doi.org/10.1016/j.cell.2017.03.003\">10.1016/j.cell.2017.03.003</a>."},"month":"03","date_created":"2018-12-11T11:47:25Z"}