{"abstract":[{"text":"The molecular and cellular mechanisms governing cell motility and directed migration in response to the chemokine SDF-1 are largely unknown. Here, we demonstrate that zebrafish primordial germ cells whose migration is guided by SDF-1 generate bleb-like protrusions that are powered by cytoplasmic flow. Protrusions are formed at sites of higher levels of free calcium where activation of myosin contraction occurs. Separation of the acto-myosin cortex from the plasma membrane at these sites is followed by a flow of cytoplasm into the forming bleb. We propose that polarized activation of the receptor CXCR4 leads to a rise in free calcium that in turn activates myosin contraction in the part of the cell responding to higher levels of the ligand SDF-1. The biased formation of new protrusions in a particular region of the cell in response to SDF-1 defines the leading edge and the direction of cell migration.","lang":"eng"}],"oa_version":"None","issue":"5","publication_status":"published","publication":"Developmental Cell","title":"Migration of zebrafish primordial germ cells: A role for myosin contraction and cytoplasmic flow","_id":"4218","doi":"10.1016/j.devcel.2006.09.023","author":[{"last_name":"Blaser","full_name":"Blaser, Heiko","first_name":"Heiko"},{"last_name":"Reichman Fried","first_name":"Michal","full_name":"Reichman Fried, Michal"},{"first_name":"Irinka","full_name":"Castanon, Irinka","last_name":"Castanon"},{"last_name":"Dumstrei","full_name":"Dumstrei, Karin","first_name":"Karin"},{"last_name":"Marlow","first_name":"Florence","full_name":"Marlow, Florence"},{"full_name":"Kawakami, Koichi","first_name":"Koichi","last_name":"Kawakami"},{"last_name":"Solnica Krezel","full_name":"Solnica Krezel, Lilianna","first_name":"Lilianna"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J"},{"last_name":"Raz","first_name":"Erez","full_name":"Raz, Erez"}],"language":[{"iso":"eng"}],"intvolume":"        11","extern":"1","type":"journal_article","publist_id":"1898","date_updated":"2021-01-12T07:55:23Z","volume":11,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2006-11-06T00:00:00Z","day":"06","year":"2006","status":"public","publisher":"Cell Press","page":"613 - 627","month":"11","date_created":"2018-12-11T12:07:39Z","citation":{"ieee":"H. Blaser <i>et al.</i>, “Migration of zebrafish primordial germ cells: A role for myosin contraction and cytoplasmic flow,” <i>Developmental Cell</i>, vol. 11, no. 5. Cell Press, pp. 613–627, 2006.","mla":"Blaser, Heiko, et al. “Migration of Zebrafish Primordial Germ Cells: A Role for Myosin Contraction and Cytoplasmic Flow.” <i>Developmental Cell</i>, vol. 11, no. 5, Cell Press, 2006, pp. 613–27, doi:<a href=\"https://doi.org/10.1016/j.devcel.2006.09.023\">10.1016/j.devcel.2006.09.023</a>.","ama":"Blaser H, Reichman Fried M, Castanon I, et al. Migration of zebrafish primordial germ cells: A role for myosin contraction and cytoplasmic flow. <i>Developmental Cell</i>. 2006;11(5):613-627. doi:<a href=\"https://doi.org/10.1016/j.devcel.2006.09.023\">10.1016/j.devcel.2006.09.023</a>","short":"H. Blaser, M. Reichman Fried, I. Castanon, K. Dumstrei, F. Marlow, K. Kawakami, L. Solnica Krezel, C.-P.J. Heisenberg, E. Raz, Developmental Cell 11 (2006) 613–627.","apa":"Blaser, H., Reichman Fried, M., Castanon, I., Dumstrei, K., Marlow, F., Kawakami, K., … Raz, E. (2006). Migration of zebrafish primordial germ cells: A role for myosin contraction and cytoplasmic flow. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2006.09.023\">https://doi.org/10.1016/j.devcel.2006.09.023</a>","ista":"Blaser H, Reichman Fried M, Castanon I, Dumstrei K, Marlow F, Kawakami K, Solnica Krezel L, Heisenberg C-PJ, Raz E. 2006. Migration of zebrafish primordial germ cells: A role for myosin contraction and cytoplasmic flow. Developmental Cell. 11(5), 613–627.","chicago":"Blaser, Heiko, Michal Reichman Fried, Irinka Castanon, Karin Dumstrei, Florence Marlow, Koichi Kawakami, Lilianna Solnica Krezel, Carl-Philipp J Heisenberg, and Erez Raz. “Migration of Zebrafish Primordial Germ Cells: A Role for Myosin Contraction and Cytoplasmic Flow.” <i>Developmental Cell</i>. Cell Press, 2006. <a href=\"https://doi.org/10.1016/j.devcel.2006.09.023\">https://doi.org/10.1016/j.devcel.2006.09.023</a>."},"article_processing_charge":"No"}