{"publication":"Development","publication_status":"published","abstract":[{"text":"We identified 6 genes that are essential for specifying ventral regions of the early zebrafish embryo, Mutations in these genes cause an expansion of structures normally derived from dorsal-lateral regions of the blastula at the expense of ventrally derived structures, A series of phenotypes of varied strengths is observed with different alleles of these mutants, The weakest phenotype is a reduction in the ventral tail fin, observed as a dominant phenotype of swirl, piggytail, and somitabun and a recessive phenotype of min fin, lost-a-fin and some piggytail alleles, With increasing phenotypic strength, the blood and pronephric anlagen are also reduced or absent, while the paraxial mesoderm and anterior neuroectoderm is progressively expanded, In the strong phenotypes, displayed by homozygous embryos of snailhouse, swirl and somitabun, the somites circle around the embryo and the midbrain region is expanded laterally, Several mutations in this group of genes are semidominant as well as recessive indicating a strong dosage sensitivity of the processes involved, Mutations in the piggytail gene display an unusual dominance that depends on both a maternal and zygotic heterozygous genotype, while somitabun is a fully penetrant dominant maternal-effect mutation, The similar and overlapping phenotypes of mutants of the 6 genes identified suggest that they function in a common pathway, which begins in oogenesis, but also depends on factors provided after the onset of zygotic transcription, presumably during blastula stages, This pathway provides ventral positional information, counteracting the dorsalizing instructions of the organizer, which is localized in the dorsal shield.","lang":"eng"}],"_id":"4170","author":[{"full_name":"Mullins, Mary","first_name":"Mary","last_name":"Mullins"},{"last_name":"Hammerschmidt","full_name":"Hammerschmidt, Matthias","first_name":"Matthias"},{"first_name":"Donald","full_name":"Kane, Donald","last_name":"Kane"},{"full_name":"Odenthal, Jörg","first_name":"Jörg","last_name":"Odenthal"},{"last_name":"Brand","first_name":"Michael","full_name":"Brand, Michael"},{"last_name":"Van Eeden","first_name":"Fredericus","full_name":"Van Eeden, Fredericus"},{"last_name":"Furutani Seiki","first_name":"Makoto","full_name":"Furutani Seiki, Makoto"},{"first_name":"Michael","full_name":"Granato, Michael","last_name":"Granato"},{"first_name":"Pascal","full_name":"Haffter, Pascal","last_name":"Haffter"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"},{"first_name":"Yunjin","full_name":"Jiang, Yunjin","last_name":"Jiang"},{"last_name":"Kelsh","full_name":"Kelsh, Robert","first_name":"Robert"},{"first_name":"Christiane","full_name":"Nüsslein Volhard, Christiane","last_name":"Nüsslein Volhard"}],"doi":"10.1242/dev.123.1.81","publication_identifier":{"issn":["0950-1991"]},"date_updated":"2022-08-05T12:01:06Z","volume":123,"extern":"1","type":"journal_article","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","status":"public","page":"81 - 93","external_id":{"pmid":["9007231"]},"citation":{"ama":"Mullins M, Hammerschmidt M, Kane D, et al. Genes establishing dorsoventral pattern formation in the zebrafish embryo: The ventral specifying genes. <i>Development</i>. 1996;123(1):81-93. doi:<a href=\"https://doi.org/10.1242/dev.123.1.81\">10.1242/dev.123.1.81</a>","mla":"Mullins, Mary, et al. “Genes Establishing Dorsoventral Pattern Formation in the Zebrafish Embryo: The Ventral Specifying Genes.” <i>Development</i>, vol. 123, no. 1, Company of Biologists, 1996, pp. 81–93, doi:<a href=\"https://doi.org/10.1242/dev.123.1.81\">10.1242/dev.123.1.81</a>.","short":"M. Mullins, M. Hammerschmidt, D. Kane, J. Odenthal, M. Brand, F. Van Eeden, M. Furutani Seiki, M. Granato, P. Haffter, C.-P.J. Heisenberg, Y. Jiang, R. Kelsh, C. Nüsslein Volhard, Development 123 (1996) 81–93.","ieee":"M. Mullins <i>et al.</i>, “Genes establishing dorsoventral pattern formation in the zebrafish embryo: The ventral specifying genes,” <i>Development</i>, vol. 123, no. 1. Company of Biologists, pp. 81–93, 1996.","chicago":"Mullins, Mary, Matthias Hammerschmidt, Donald Kane, Jörg Odenthal, Michael Brand, Fredericus Van Eeden, Makoto Furutani Seiki, et al. “Genes Establishing Dorsoventral Pattern Formation in the Zebrafish Embryo: The Ventral Specifying Genes.” <i>Development</i>. Company of Biologists, 1996. <a href=\"https://doi.org/10.1242/dev.123.1.81\">https://doi.org/10.1242/dev.123.1.81</a>.","ista":"Mullins M, Hammerschmidt M, Kane D, Odenthal J, Brand M, Van Eeden F, Furutani Seiki M, Granato M, Haffter P, Heisenberg C-PJ, Jiang Y, Kelsh R, Nüsslein Volhard C. 1996. Genes establishing dorsoventral pattern formation in the zebrafish embryo: The ventral specifying genes. Development. 123(1), 81–93.","apa":"Mullins, M., Hammerschmidt, M., Kane, D., Odenthal, J., Brand, M., Van Eeden, F., … Nüsslein Volhard, C. (1996). Genes establishing dorsoventral pattern formation in the zebrafish embryo: The ventral specifying genes. <i>Development</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/dev.123.1.81\">https://doi.org/10.1242/dev.123.1.81</a>"},"month":"12","date_created":"2018-12-11T12:07:22Z","acknowledgement":"We would like to thank: Eric Weinberg, and David Ransom and Leonard Zon for providing the myoD and gata1 cDNA clone, respectively, prior to publication; David Ransom for pointing out the histological blood staining method; J. S. Joly for the eve1 cDNA clone; Mary Ellen Lane, Siegfried Roth, Stefan Schulte-Merker, Herbert Steinbeiser for helpful comments on the manuscript; and very special thanks to Karin Finger-Miller for technical support, as well as to Hans-Martin Maischein, Amanda Wilson, Jörg Zeller, and Cosima Fabian. This work was supported by an NIH postdoctoral fellowship to M. C. M.","article_processing_charge":"No","issue":"1","oa_version":"None","scopus_import":"1","quality_controlled":"1","title":"Genes establishing dorsoventral pattern formation in the zebrafish embryo: The ventral specifying genes","intvolume":"       123","language":[{"iso":"eng"}],"publist_id":"1951","date_published":"1996-12-01T00:00:00Z","publisher":"Company of Biologists","year":"1996","article_type":"original","day":"01","pmid":1}