{"date_updated":"2024-02-27T10:03:37Z","volume":13,"extern":"1","type":"journal_article","publication_identifier":{"eissn":["1879-0445"],"issn":["0960-9822"]},"_id":"4169","author":[{"full_name":"Montero, Juan","first_name":"Juan","last_name":"Montero"},{"first_name":"Beate","full_name":"Kilian, Beate","last_name":"Kilian"},{"last_name":"Chan","first_name":"Joanne","full_name":"Chan, Joanne"},{"last_name":"Bayliss","full_name":"Bayliss, Peter","first_name":"Peter"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"doi":"10.1016/S0960-9822(03)00505-0","publication_status":"published","publication":"Current Biology","abstract":[{"lang":"eng","text":"Background: During vertebrate gastrulation, cell polarization and migration are core components in the cellular rearrangements that lead to the formation of the three germ layers, ectoderm, mesoderm, and endoderm. Previous studies have implicated the Wnt/planar cell polarity (PCP) signaling pathway in controlling cell morphology and movement during gastrulation. However, cell polarization and directed cell migration are reduced but not completely abolished in the absence of Wnt/PCP signals; this observation indicates that other signaling pathways must be involved. Results: We show that Phosphoinositide 3-Kinases (PI3Ks) are required at the onset of zebrafish gastrulation in mesendodermal cells for process formation and cell polarization. Platelet Derived Growth Factor (PDGF) functions upstream of PI3K, while Protein Kinase B (PKB), a downstream effector of PI3K activity, localizes to the leading edge of migrating mesendodermal cells. In the absence of PI3K activity, PKB localization and cell polarization are strongly reduced in mesendodermal cells and are followed by slower but still highly coordinated and directed movements of these cells. Conclusions: We have identified a novel role of a signaling pathway comprised of PDGF, PI3K, and PKB in the control of morphogenetic cell movements during gastrulation. Furthermore, our findings provide insight into the relationship between cell polarization and directed cell migration at the onset of zebrafish gastrulation."}],"acknowledgement":"We would like to thank Jennifer Geiger, Juan Hurl& Hannu Mansu-koski, Florian Raible, Marino Zerial, Steve Wilson, and Kurt Anderson for critical reading of earlier versions of this manuscript. We thank Erez Raz, Bart Vanhaesebroeck, and Lukas Roth for sending us the pCS2-PH-GFP-nos, the p1IOCAAX, and the pCS2-actin-GFP constructs, respectively. We are grateful to Marino Zerial and his lab for encouraging us to start this work and providing us with the dnP13K construct and to Florian Ulrich and Franziska Friedrich for help with the confocal microscope and artwork, respectively. We thank Gunter Junghanns and Evelyn Lehmann for excellent fish care. C.-P.H. is supported by an Emmy-Noother-Fellowship from the Deutsche Forschungsgemeinschaft. ","article_processing_charge":"No","citation":{"mla":"Montero, Juan, et al. “Phosphoinositide 3-Kinase Is Required for Process Outgrowth and Cell Polarization of Gastrulating Mesendodermal Cells.” <i>Current Biology</i>, vol. 13, no. 15, Cell Press, 2003, pp. 1279–89, doi:<a href=\"https://doi.org/10.1016/S0960-9822(03)00505-0\">10.1016/S0960-9822(03)00505-0</a>.","ama":"Montero J, Kilian B, Chan J, Bayliss P, Heisenberg C-PJ. Phosphoinositide 3-kinase is required for process outgrowth and cell polarization of gastrulating mesendodermal cells. <i>Current Biology</i>. 2003;13(15):1279-1289. doi:<a href=\"https://doi.org/10.1016/S0960-9822(03)00505-0\">10.1016/S0960-9822(03)00505-0</a>","short":"J. Montero, B. Kilian, J. Chan, P. Bayliss, C.-P.J. Heisenberg, Current Biology 13 (2003) 1279–1289.","ieee":"J. Montero, B. Kilian, J. Chan, P. Bayliss, and C.-P. J. Heisenberg, “Phosphoinositide 3-kinase is required for process outgrowth and cell polarization of gastrulating mesendodermal cells,” <i>Current Biology</i>, vol. 13, no. 15. Cell Press, pp. 1279–1289, 2003.","ista":"Montero J, Kilian B, Chan J, Bayliss P, Heisenberg C-PJ. 2003. Phosphoinositide 3-kinase is required for process outgrowth and cell polarization of gastrulating mesendodermal cells. Current Biology. 13(15), 1279–1289.","chicago":"Montero, Juan, Beate Kilian, Joanne Chan, Peter Bayliss, and Carl-Philipp J Heisenberg. “Phosphoinositide 3-Kinase Is Required for Process Outgrowth and Cell Polarization of Gastrulating Mesendodermal Cells.” <i>Current Biology</i>. Cell Press, 2003. <a href=\"https://doi.org/10.1016/S0960-9822(03)00505-0\">https://doi.org/10.1016/S0960-9822(03)00505-0</a>.","apa":"Montero, J., Kilian, B., Chan, J., Bayliss, P., &#38; Heisenberg, C.-P. J. (2003). Phosphoinositide 3-kinase is required for process outgrowth and cell polarization of gastrulating mesendodermal cells. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/S0960-9822(03)00505-0\">https://doi.org/10.1016/S0960-9822(03)00505-0</a>"},"month":"08","date_created":"2018-12-11T12:07:22Z","status":"public","page":"1279 - 1289","external_id":{"pmid":[" 12906787"]},"user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","publist_id":"1950","intvolume":"        13","language":[{"iso":"eng"}],"quality_controlled":"1","scopus_import":"1","title":"Phosphoinositide 3-kinase is required for process outgrowth and cell polarization of gastrulating mesendodermal cells","issue":"15","oa_version":"None","pmid":1,"year":"2003","article_type":"original","publisher":"Cell Press","day":"05","date_published":"2003-08-05T00:00:00Z"}