{"abstract":[{"lang":"eng","text":"Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells."}],"publication_status":"published","publication":"Nature Methods","issue":"7","quality_controlled":0,"title":"Lifeact: a versatile marker to visualize F-actin","_id":"3944","author":[{"first_name":"Julia","full_name":"Riedl, Julia","last_name":"Riedl"},{"last_name":"Crevenna","first_name":"Alvaro","full_name":"Crevenna, Alvaro H"},{"first_name":"Kai","full_name":"Kessenbrock, Kai","last_name":"Kessenbrock"},{"first_name":"Jerry","full_name":"Yu, Jerry Haochen","last_name":"Yu"},{"last_name":"Neukirchen","first_name":"Dorothee","full_name":"Neukirchen, Dorothee"},{"last_name":"Bista","full_name":"Bista, Michal","first_name":"Michal"},{"first_name":"Frank","full_name":"Bradke, Frank","last_name":"Bradke"},{"last_name":"Jenne","first_name":"Dieter","full_name":"Jenne, Dieter"},{"last_name":"Holak","full_name":"Holak, Tad A","first_name":"Tad"},{"first_name":"Zena","full_name":"Werb, Zena","last_name":"Werb"},{"first_name":"Michael K","full_name":"Michael Sixt","orcid":"0000-0002-6620-9179","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Wedlich Soldner","first_name":"Roland","full_name":"Wedlich-Soldner, Roland"}],"doi":"10.1038/nmeth.1220","intvolume":"         5","extern":1,"type":"journal_article","date_updated":"2021-01-12T07:53:22Z","publist_id":"2184","volume":5,"date_published":"2008-06-08T00:00:00Z","day":"08","status":"public","publisher":"Nature Publishing Group","year":"2008","page":"605 - 607","month":"06","date_created":"2018-12-11T12:06:02Z","citation":{"mla":"Riedl, Julia, et al. “Lifeact: A Versatile Marker to Visualize F-Actin.” <i>Nature Methods</i>, vol. 5, no. 7, Nature Publishing Group, 2008, pp. 605–07, doi:<a href=\"https://doi.org/10.1038/nmeth.1220\">10.1038/nmeth.1220</a>.","ama":"Riedl J, Crevenna A, Kessenbrock K, et al. Lifeact: a versatile marker to visualize F-actin. <i>Nature Methods</i>. 2008;5(7):605-607. doi:<a href=\"https://doi.org/10.1038/nmeth.1220\">10.1038/nmeth.1220</a>","short":"J. Riedl, A. Crevenna, K. Kessenbrock, J. Yu, D. Neukirchen, M. Bista, F. Bradke, D. Jenne, T. Holak, Z. Werb, M.K. Sixt, R. Wedlich Soldner, Nature Methods 5 (2008) 605–607.","ieee":"J. Riedl <i>et al.</i>, “Lifeact: a versatile marker to visualize F-actin,” <i>Nature Methods</i>, vol. 5, no. 7. Nature Publishing Group, pp. 605–607, 2008.","ista":"Riedl J, Crevenna A, Kessenbrock K, Yu J, Neukirchen D, Bista M, Bradke F, Jenne D, Holak T, Werb Z, Sixt MK, Wedlich Soldner R. 2008. Lifeact: a versatile marker to visualize F-actin. Nature Methods. 5(7), 605–607.","chicago":"Riedl, Julia, Alvaro Crevenna, Kai Kessenbrock, Jerry Yu, Dorothee Neukirchen, Michal Bista, Frank Bradke, et al. “Lifeact: A Versatile Marker to Visualize F-Actin.” <i>Nature Methods</i>. Nature Publishing Group, 2008. <a href=\"https://doi.org/10.1038/nmeth.1220\">https://doi.org/10.1038/nmeth.1220</a>.","apa":"Riedl, J., Crevenna, A., Kessenbrock, K., Yu, J., Neukirchen, D., Bista, M., … Wedlich Soldner, R. (2008). Lifeact: a versatile marker to visualize F-actin. <i>Nature Methods</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nmeth.1220\">https://doi.org/10.1038/nmeth.1220</a>"}}