{"date_published":"2001-05-21T00:00:00Z","year":"2001","article_type":"original","publisher":"Rockefeller University Press","day":"21","pmid":1,"issue":"5","oa_version":"Published Version","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174323/"}],"quality_controlled":"1","scopus_import":"1","title":"Endothelial cell laminin isoforms, laminins 8 and 10, play decisive roles in T cell recruitment across the blood-brain barrier in experimental autoimmune encephalomyelitis","intvolume":"       153","language":[{"iso":"eng"}],"publist_id":"2198","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","oa":1,"status":"public","page":"933 - 946","external_id":{"pmid":["11381080"]},"citation":{"chicago":"Sixt, Michael K, Britta Engelhardt, Friederike Pausch, Rupert Hallmann, Olaf Wendler, and Lydia Sorokin. “Endothelial Cell Laminin Isoforms, Laminins 8 and 10, Play Decisive Roles in T Cell Recruitment across the Blood-Brain Barrier in Experimental Autoimmune Encephalomyelitis.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2001. <a href=\"https://doi.org/10.1083/jcb.153.5.933 \">https://doi.org/10.1083/jcb.153.5.933 </a>.","ista":"Sixt MK, Engelhardt B, Pausch F, Hallmann R, Wendler O, Sorokin L. 2001. Endothelial cell laminin isoforms, laminins 8 and 10, play decisive roles in T cell recruitment across the blood-brain barrier in experimental autoimmune encephalomyelitis. Journal of Cell Biology. 153(5), 933–946.","apa":"Sixt, M. K., Engelhardt, B., Pausch, F., Hallmann, R., Wendler, O., &#38; Sorokin, L. (2001). Endothelial cell laminin isoforms, laminins 8 and 10, play decisive roles in T cell recruitment across the blood-brain barrier in experimental autoimmune encephalomyelitis. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.153.5.933 \">https://doi.org/10.1083/jcb.153.5.933 </a>","short":"M.K. Sixt, B. Engelhardt, F. Pausch, R. Hallmann, O. Wendler, L. Sorokin, Journal of Cell Biology 153 (2001) 933–946.","mla":"Sixt, Michael K., et al. “Endothelial Cell Laminin Isoforms, Laminins 8 and 10, Play Decisive Roles in T Cell Recruitment across the Blood-Brain Barrier in Experimental Autoimmune Encephalomyelitis.” <i>Journal of Cell Biology</i>, vol. 153, no. 5, Rockefeller University Press, 2001, pp. 933–46, doi:<a href=\"https://doi.org/10.1083/jcb.153.5.933 \">10.1083/jcb.153.5.933 </a>.","ama":"Sixt MK, Engelhardt B, Pausch F, Hallmann R, Wendler O, Sorokin L. Endothelial cell laminin isoforms, laminins 8 and 10, play decisive roles in T cell recruitment across the blood-brain barrier in experimental autoimmune encephalomyelitis. <i>Journal of Cell Biology</i>. 2001;153(5):933-946. doi:<a href=\"https://doi.org/10.1083/jcb.153.5.933 \">10.1083/jcb.153.5.933 </a>","ieee":"M. K. Sixt, B. Engelhardt, F. Pausch, R. Hallmann, O. Wendler, and L. Sorokin, “Endothelial cell laminin isoforms, laminins 8 and 10, play decisive roles in T cell recruitment across the blood-brain barrier in experimental autoimmune encephalomyelitis,” <i>Journal of Cell Biology</i>, vol. 153, no. 5. Rockefeller University Press, pp. 933–946, 2001."},"month":"05","date_created":"2018-12-11T12:05:57Z","acknowledgement":"The authors thank Stefanie Karosi for careful and critical reading of the manuscript and Monika Bruckner for expert technical assistance. We are particularly grateful to Winfried Neuhuber for help with confocal microscopy and interpretation of the data. This work was supported by Deutsche Forschungsgemeinschaft grants So285/1-3 and So285/1-4 to L.M. Sorokin.","article_processing_charge":"No","publication":"Journal of Cell Biology","publication_status":"published","abstract":[{"text":"An active involvement of blood-brain barrier endothelial cell basement membranes in development of inflammatory lesions in the central nervous system (CNS) has not been considered to date. Here we investigated the molecular composition and possible function of the extracellular matrix encountered by extravasating T lymphocytes during experimental autoimmune encephalomyelitis (EAE). Endothelial basement membranes contained laminin 8 (alpha4beta1gamma1) and/or 10 (alpha5beta1gamma1) and their expression was influenced by proinflammatory cytokines or angiostatic agents. T cells emigrating into the CNS during EAE encountered two biochemically distinct basement membranes, the endothelial (containing laminins 8 and 10) and the parenchymal (containing laminins 1 and 2) basement membranes. However, inflammatory cuffs occurred exclusively around endothelial basement membranes containing laminin 8, whereas in the presence of laminin 10 no infiltration was detectable. In vitro assays using encephalitogenic T cell lines revealed adhesion to laminins 8 and 10, whereas binding to laminins 1 and 2 could not be induced. Downregulation of integrin alpha6 on cerebral endothelium at sites of T cell infiltration, plus a high turnover of laminin 8 at these sites, suggested two possible roles for laminin 8 in the endothelial basement membrane: one at the level of the endothelial cells resulting in reduced adhesion and, thereby, increased penetrability of the monolayer; and secondly at the level of the T cells providing direct signals to the transmigrating cells.","lang":"eng"}],"_id":"3930","author":[{"full_name":"Sixt, Michael K","first_name":"Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Engelhardt","first_name":"Britta","full_name":"Engelhardt, Britta"},{"first_name":"Friederike","full_name":"Pausch, Friederike","last_name":"Pausch"},{"full_name":"Hallmann, Rupert","first_name":"Rupert","last_name":"Hallmann"},{"last_name":"Wendler","first_name":"Olaf","full_name":"Wendler, Olaf"},{"full_name":"Sorokin, Lydia","first_name":"Lydia","last_name":"Sorokin"}],"doi":"10.1083/jcb.153.5.933 ","publication_identifier":{"issn":["0021-9525"]},"date_updated":"2023-05-11T12:19:36Z","volume":153,"extern":"1","type":"journal_article"}