{"intvolume":"       586","volume":586,"publist_id":"2386","date_updated":"2021-01-12T07:52:28Z","type":"journal_article","extern":1,"publication_status":"published","issue":"8","publication":"Journal of Physiology","abstract":[{"text":"Fast-spiking parvalbumin-expressing basket cells (BCs) represent a major type of inhibitory interneuron in the hippocampus. These cells inhibit principal cells in a temporally precise manner and are involved in the generation of network oscillations. Although BCs show a unique expression profile of Ca(2+)-permeable receptors, Ca(2+)-binding proteins and Ca(2+)-dependent signalling molecules, physiological Ca(2+) signalling in these interneurons has not been investigated. To study action potential (AP)-induced dendritic Ca(2+) influx and buffering, we combined whole-cell patch-clamp recordings with ratiometric Ca(2+) imaging from the proximal apical dendrites of rigorously identified BCs in acute slices, using the high-affinity Ca(2+) indicator fura-2 or the low-affinity dye fura-FF. Single APs evoked dendritic Ca(2+) transients with small amplitude. Bursts of APs evoked Ca(2+) transients with amplitudes that increased linearly with AP number. Analysis of Ca(2+) transients under steady-state conditions with different fura-2 concentrations and during loading with 200 microm fura-2 indicated that the endogenous Ca(2+)-binding ratio was approximately 200 (kappa(S) = 202 +/- 26 for the loading experiments). The peak amplitude of the Ca(2+) transients measured directly with 100 microm fura-FF was 39 nm AP(-1). At approximately 23 degrees C, the decay time constant of the Ca(2+) transients was 390 ms, corresponding to an extrusion rate of approximately 600 s(-1). At 34 degrees C, the decay time constant was 203 ms and the corresponding extrusion rate was approximately 1100 s(-1). At both temperatures, continuous theta-burst activity with three to five APs per theta cycle, as occurs in vivo during exploration, led to a moderate increase in the global Ca(2+) concentration that was proportional to AP number, whereas more intense stimulation was required to reach micromolar Ca(2+) concentrations and to shift Ca(2+) signalling into a non-linear regime. In conclusion, dentate gyrus BCs show a high endogenous Ca(2+)-binding ratio, a small AP-induced dendritic Ca(2+) influx, and a relatively slow Ca(2+) extrusion. These specific buffering properties of BCs will sharpen the time course of local Ca(2+) signals, while prolonging the decay of global Ca(2+) signals.","lang":"eng"}],"doi":"10.1113/jphysiol.2007.147298","author":[{"last_name":"Aponte","full_name":"Aponte, Yexica","first_name":"Yexica"},{"first_name":"Josef","full_name":"Bischofberger, Josef","last_name":"Bischofberger"},{"last_name":"Jonas","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","full_name":"Peter Jonas","first_name":"Peter M","orcid":"0000-0001-5001-4804"}],"_id":"3825","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2465201/"}],"title":"Efficient Ca(2+) buffering in fast-spiking basket cells of rat hippocampus","quality_controlled":0,"citation":{"apa":"Aponte, Y., Bischofberger, J., &#38; Jonas, P. M. (2008). Efficient Ca(2+) buffering in fast-spiking basket cells of rat hippocampus. <i>Journal of Physiology</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1113/jphysiol.2007.147298\">https://doi.org/10.1113/jphysiol.2007.147298</a>","ista":"Aponte Y, Bischofberger J, Jonas PM. 2008. Efficient Ca(2+) buffering in fast-spiking basket cells of rat hippocampus. Journal of Physiology. 586(8), 2061–75.","chicago":"Aponte, Yexica, Josef Bischofberger, and Peter M Jonas. “Efficient Ca(2+) Buffering in Fast-Spiking Basket Cells of Rat Hippocampus.” <i>Journal of Physiology</i>. Wiley-Blackwell, 2008. <a href=\"https://doi.org/10.1113/jphysiol.2007.147298\">https://doi.org/10.1113/jphysiol.2007.147298</a>.","ieee":"Y. Aponte, J. Bischofberger, and P. M. Jonas, “Efficient Ca(2+) buffering in fast-spiking basket cells of rat hippocampus,” <i>Journal of Physiology</i>, vol. 586, no. 8. Wiley-Blackwell, pp. 2061–75, 2008.","ama":"Aponte Y, Bischofberger J, Jonas PM. Efficient Ca(2+) buffering in fast-spiking basket cells of rat hippocampus. <i>Journal of Physiology</i>. 2008;586(8):2061-2075. doi:<a href=\"https://doi.org/10.1113/jphysiol.2007.147298\">10.1113/jphysiol.2007.147298</a>","mla":"Aponte, Yexica, et al. “Efficient Ca(2+) Buffering in Fast-Spiking Basket Cells of Rat Hippocampus.” <i>Journal of Physiology</i>, vol. 586, no. 8, Wiley-Blackwell, 2008, pp. 2061–75, doi:<a href=\"https://doi.org/10.1113/jphysiol.2007.147298\">10.1113/jphysiol.2007.147298</a>.","short":"Y. Aponte, J. Bischofberger, P.M. Jonas, Journal of Physiology 586 (2008) 2061–75."},"date_created":"2018-12-11T12:05:22Z","month":"01","date_published":"2008-01-01T00:00:00Z","oa":1,"page":"2061 - 75","publisher":"Wiley-Blackwell","year":"2008","status":"public","day":"01"}