{"quality_controlled":0,"title":"Differential gating and recruitment of P/Q-, N-, and R-type Ca(2+) channels in hippocampal mossy fiber boutons","_id":"3819","doi":"10.1523/JNEUROSCI.1709-07.2007","author":[{"full_name":"Li, Liyi","first_name":"Liyi","last_name":"Li"},{"full_name":"Bischofberger, Josef","first_name":"Josef","last_name":"Bischofberger"},{"full_name":"Peter Jonas","first_name":"Peter M","orcid":"0000-0001-5001-4804","last_name":"Jonas","id":"353C1B58-F248-11E8-B48F-1D18A9856A87"}],"abstract":[{"text":"Voltage-gated Ca2+ channels in presynaptic terminals initiate the Ca2+ inflow necessary for transmitter release. At a variety of synapses, multiple Ca2+ channel subtypes are involved in synaptic transmission and plasticity. However, it is unknown whether presynaptic Ca2+ channels differ in gating properties and whether they are differentially activated by action potentials or subthreshold voltage signals. We examined Ca2+ channels in hippocampal mossy fiber boutons (MFBs) by presynaptic recording, using the selective blockers omega-agatoxin IVa, omega-conotoxin GVIa, and SNX-482 to separate P/Q-, N-, and R-type components. Nonstationary fluctuation analysis combined with blocker application revealed a single MFB contained on average approximately 2000 channels, with 66% P/Q-, 26% N-, and 8% R-type channels. Whereas both P/Q-type and N-type Ca2+ channels showed high activation threshold and rapid activation and deactivation, R-type Ca2+ channels had a lower activation threshold and slower gating kinetics. To determine the efficacy of activation of different Ca2+ channel subtypes by physiologically relevant voltage waveforms, a six-state gating model reproducing the experimental observations was developed. Action potentials activated P/Q-type Ca2+ channels with high efficacy, whereas N- and R-type channels were activated less efficiently. Action potential broadening selectively recruited N- and R-type channels, leading to an equalization of the efficacy of channel activation. In contrast, subthreshold presynaptic events activated R-type channels more efficiently than P/Q- or N-type channels. In conclusion, single MFBs coexpress multiple types of Ca2+ channels, which are activated differentially by subthreshold and suprathreshold presynaptic voltage signals.","lang":"eng"}],"issue":"49","publication":"Journal of Neuroscience","publication_status":"published","extern":1,"type":"journal_article","publist_id":"2389","date_updated":"2021-01-12T07:52:25Z","volume":27,"intvolume":"        27","day":"01","publisher":"Society for Neuroscience","year":"2007","status":"public","page":"13420 - 9","date_published":"2007-01-01T00:00:00Z","month":"01","date_created":"2018-12-11T12:05:20Z","citation":{"short":"L. Li, J. Bischofberger, P.M. Jonas, Journal of Neuroscience 27 (2007) 13420–9.","ama":"Li L, Bischofberger J, Jonas PM. Differential gating and recruitment of P/Q-, N-, and R-type Ca(2+) channels in hippocampal mossy fiber boutons. <i>Journal of Neuroscience</i>. 2007;27(49):13420-13429. doi:<a href=\"https://doi.org/10.1523/JNEUROSCI.1709-07.2007\">10.1523/JNEUROSCI.1709-07.2007</a>","mla":"Li, Liyi, et al. “Differential Gating and Recruitment of P/Q-, N-, and R-Type Ca(2+) Channels in Hippocampal Mossy Fiber Boutons.” <i>Journal of Neuroscience</i>, vol. 27, no. 49, Society for Neuroscience, 2007, pp. 13420–29, doi:<a href=\"https://doi.org/10.1523/JNEUROSCI.1709-07.2007\">10.1523/JNEUROSCI.1709-07.2007</a>.","ieee":"L. Li, J. Bischofberger, and P. M. Jonas, “Differential gating and recruitment of P/Q-, N-, and R-type Ca(2+) channels in hippocampal mossy fiber boutons,” <i>Journal of Neuroscience</i>, vol. 27, no. 49. Society for Neuroscience, pp. 13420–9, 2007.","ista":"Li L, Bischofberger J, Jonas PM. 2007. Differential gating and recruitment of P/Q-, N-, and R-type Ca(2+) channels in hippocampal mossy fiber boutons. Journal of Neuroscience. 27(49), 13420–9.","chicago":"Li, Liyi, Josef Bischofberger, and Peter M Jonas. “Differential Gating and Recruitment of P/Q-, N-, and R-Type Ca(2+) Channels in Hippocampal Mossy Fiber Boutons.” <i>Journal of Neuroscience</i>. Society for Neuroscience, 2007. <a href=\"https://doi.org/10.1523/JNEUROSCI.1709-07.2007\">https://doi.org/10.1523/JNEUROSCI.1709-07.2007</a>.","apa":"Li, L., Bischofberger, J., &#38; Jonas, P. M. (2007). Differential gating and recruitment of P/Q-, N-, and R-type Ca(2+) channels in hippocampal mossy fiber boutons. <i>Journal of Neuroscience</i>. Society for Neuroscience. <a href=\"https://doi.org/10.1523/JNEUROSCI.1709-07.2007\">https://doi.org/10.1523/JNEUROSCI.1709-07.2007</a>"}}