{"_id":"3750","abstract":[{"lang":"eng","text":"We applied a single-cell assay to characterize how transcription dynamics affects protein expression levels of a tetracycline-inducible gene expression system. Transcriptional activity of the tetracycline promoter in response to a steady level of inducer is steady in ΔacrAB efflux mutant but pulsating in wildtype Escherichia coli cells. We found that the expression level of the green fluorescent protein is several folds higher in ΔacrAB efflux mutant than in wildtype cells."}],"publication_status":"published","citation":{"mla":"Le, Thuc, et al. “Protein Expression Enhancement in Efflux-Deleted Mutant Bacteria.” <i>Protein Expression and Purification</i>, vol. 48, no. 1, Elsevier, 2006, pp. 28–31.","ieee":"T. Le, C. C. Guet, and P. Cluzel, “Protein expression enhancement in efflux-deleted mutant bacteria,” <i>Protein Expression and Purification</i>, vol. 48, no. 1. Elsevier, pp. 28–31, 2006.","ista":"Le T, Guet CC, Cluzel P. 2006. Protein expression enhancement in efflux-deleted mutant bacteria. Protein Expression and Purification. 48(1), 28–31.","chicago":"Le, Thuc, Calin C Guet, and Philippe Cluzel. “Protein Expression Enhancement in Efflux-Deleted Mutant Bacteria.” <i>Protein Expression and Purification</i>. Elsevier, 2006.","apa":"Le, T., Guet, C. C., &#38; Cluzel, P. (2006). Protein expression enhancement in efflux-deleted mutant bacteria. <i>Protein Expression and Purification</i>. Elsevier.","ama":"Le T, Guet CC, Cluzel P. Protein expression enhancement in efflux-deleted mutant bacteria. <i>Protein Expression and Purification</i>. 2006;48(1):28-31.","short":"T. Le, C.C. Guet, P. Cluzel, Protein Expression and Purification 48 (2006) 28–31."},"date_published":"2006-07-01T00:00:00Z","extern":1,"issue":"1","month":"07","date_updated":"2021-01-12T07:51:56Z","type":"journal_article","author":[{"first_name":"Thuc","last_name":"Le","full_name":"Le,Thuc T."},{"orcid":"0000-0001-6220-2052","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","full_name":"Calin Guet","last_name":"Guet","first_name":"Calin C"},{"last_name":"Cluzel","first_name":"Philippe","full_name":"Cluzel,Philippe"}],"day":"01","publication":"Protein Expression and Purification","page":"28 - 31","quality_controlled":0,"title":"Protein expression enhancement in efflux-deleted mutant bacteria","status":"public","year":"2006","publisher":"Elsevier","intvolume":"        48","volume":48,"date_created":"2018-12-11T12:04:58Z","publist_id":"2478"}