{"issue":"1","extern":"1","publication_status":"published","date_published":"1998-05-01T00:00:00Z","abstract":[{"lang":"eng","text":"Determining the way in which deleterious mutations interact in their effects on fitness is crucial to numerous areas in population genetics and evolutionary biology. For example, if each additional mutation leads to a greater decrease in log fitness than the last (synergistic epistasis), then the evolution of sex and recombination may be favored to facilitate the elimination of deleterious mutations. However, there is a severe shortage of relevant data. Three relatively simple experimental methods to test for epistasis between deleterious mutations in haploid species have recently been proposed. These methods involve crossing individuals and examining the mean and/or skew in log fitness of the offspring and parents. The main aim of this paper is to formalize these methods, and determine the most effective way in which tests for epistasis could be carried out. We show that only one of these methods is likely to give useful results: crossing individuals that have very different numbers of deleterious mutations, and comparing the mean log fitness of the parents with that of their offspring. We also reconsider experimental data collected on Chlamydomonas moewussi using two of the three methods. Finally, we suggest how the test could be applied to diploid species."}],"main_file_link":[{"url":"https://academic.oup.com/genetics/article/149/1/435/6034229","open_access":"1"}],"day":"01","type":"journal_article","pmid":1,"date_updated":"2022-08-29T08:53:09Z","month":"05","article_processing_charge":"No","status":"public","quality_controlled":"1","title":"Testing for epistasis between deleterious mutations","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","date_created":"2018-12-11T12:04:19Z","oa":1,"oa_version":"None","scopus_import":"1","publisher":"Genetics Society of America","article_type":"original","citation":{"short":"S. West, A. Peters, N.H. Barton, Genetics 149 (1998) 435–444.","ama":"West S, Peters A, Barton NH. Testing for epistasis between deleterious mutations. <i>Genetics</i>. 1998;149(1):435-444. doi:<a href=\"https://doi.org/10.1093/genetics/149.1.435\">10.1093/genetics/149.1.435</a>","chicago":"West, Stuart, Andrew Peters, and Nicholas H Barton. “Testing for Epistasis between Deleterious Mutations.” <i>Genetics</i>. Genetics Society of America, 1998. <a href=\"https://doi.org/10.1093/genetics/149.1.435\">https://doi.org/10.1093/genetics/149.1.435</a>.","ista":"West S, Peters A, Barton NH. 1998. Testing for epistasis between deleterious mutations. Genetics. 149(1), 435–444.","apa":"West, S., Peters, A., &#38; Barton, N. H. (1998). Testing for epistasis between deleterious mutations. <i>Genetics</i>. Genetics Society of America. <a href=\"https://doi.org/10.1093/genetics/149.1.435\">https://doi.org/10.1093/genetics/149.1.435</a>","ieee":"S. West, A. Peters, and N. H. Barton, “Testing for epistasis between deleterious mutations,” <i>Genetics</i>, vol. 149, no. 1. Genetics Society of America, pp. 435–444, 1998.","mla":"West, Stuart, et al. “Testing for Epistasis between Deleterious Mutations.” <i>Genetics</i>, vol. 149, no. 1, Genetics Society of America, 1998, pp. 435–44, doi:<a href=\"https://doi.org/10.1093/genetics/149.1.435\">10.1093/genetics/149.1.435</a>."},"language":[{"iso":"eng"}],"_id":"3628","doi":"10.1093/genetics/149.1.435","page":"435 - 444","author":[{"full_name":"West, Stuart","first_name":"Stuart","last_name":"West"},{"full_name":"Peters, Andrew","last_name":"Peters","first_name":"Andrew"},{"full_name":"Barton, Nicholas H","id":"4880FE40-F248-11E8-B48F-1D18A9856A87","last_name":"Barton","first_name":"Nicholas H","orcid":"0000-0002-8548-5240"}],"publication":"Genetics","publication_identifier":{"issn":["0016-6731"]},"external_id":{"pmid":["9584115"]},"acknowledgement":"We thank BRIAN  CHARLESWORTH, ANDREW  CLARK, LAURENCE  HURST, PETER  KEIGHTLEY, ALEXEY  KONDRASHOV, CURT  LIVELY, MARGARET  MACKINNON, KATRINA  LYTHGOE, SALLY  OTTO, ANDREW  READ and ARJAN DE  VISSER for useful discussion and comments on the manuscript. This work was supported by the Biotechnology and Biological Sciences Research Council.","volume":149,"publist_id":"2755","intvolume":"       149","year":"1998"}