{"pmid":1,"publisher":"Elsevier","article_type":"original","year":"1995","day":"01","date_published":"1995-07-01T00:00:00Z","publist_id":"2907","intvolume":"        15","language":[{"iso":"eng"}],"main_file_link":[{"url":"https://www.sciencedirect.com/science/article/pii/0896627395900764?via%3Dihub","open_access":"1"}],"title":"Relative abundance of subunit mRNAs determines gating and Ca(2+) permeability of AMPA receptors in principal neurons and interneurons in rat CNS","quality_controlled":"1","scopus_import":"1","issue":"1","oa_version":"Published Version","article_processing_charge":"No","acknowledgement":"We thank Ulla Amtmann for efficient help with the molecular analysis. We also thank M. Kaiser for technical assistance, Dr. J. G. G. Borst for advice concerning preparation of brainstem slices, and Drs. N. Spruston and G. Stuart for critically reading the manuscript. Funded in part by Bundesministerium für Forschung und Technologie grant BCT 364 AZ 321/7291 (P. H. S.) and by Deutsche Forschungsgemeinschaftgrant SFB-3171814(P. J.). J. R. P. G. and T. M. were supported by the graduate program of Molecular and Cellular Neurobiology of the University of Heidelberg. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby\r\nmarked “advertisement” in accordance with 18 USC Section 1734 solely to Indicate this fact.","citation":{"ieee":"J. Geiger <i>et al.</i>, “Relative abundance of subunit mRNAs determines gating and Ca(2+) permeability of AMPA receptors in principal neurons and interneurons in rat CNS,” <i>Neuron</i>, vol. 15, no. 1. Elsevier, pp. 193–204, 1995.","short":"J. Geiger, T. Melcher, D. Koh, B. Sakmann, P. Seeburg, P.M. Jonas, H. Monyer, Neuron 15 (1995) 193–204.","mla":"Geiger, Jörg, et al. “Relative Abundance of Subunit MRNAs Determines Gating and Ca(2+) Permeability of AMPA Receptors in Principal Neurons and Interneurons in Rat CNS.” <i>Neuron</i>, vol. 15, no. 1, Elsevier, 1995, pp. 193–204, doi:<a href=\"https://doi.org/10.1016/0896-6273(95)90076-4\">10.1016/0896-6273(95)90076-4</a>.","ama":"Geiger J, Melcher T, Koh D, et al. Relative abundance of subunit mRNAs determines gating and Ca(2+) permeability of AMPA receptors in principal neurons and interneurons in rat CNS. <i>Neuron</i>. 1995;15(1):193-204. doi:<a href=\"https://doi.org/10.1016/0896-6273(95)90076-4\">10.1016/0896-6273(95)90076-4</a>","apa":"Geiger, J., Melcher, T., Koh, D., Sakmann, B., Seeburg, P., Jonas, P. M., &#38; Monyer, H. (1995). Relative abundance of subunit mRNAs determines gating and Ca(2+) permeability of AMPA receptors in principal neurons and interneurons in rat CNS. <i>Neuron</i>. Elsevier. <a href=\"https://doi.org/10.1016/0896-6273(95)90076-4\">https://doi.org/10.1016/0896-6273(95)90076-4</a>","ista":"Geiger J, Melcher T, Koh D, Sakmann B, Seeburg P, Jonas PM, Monyer H. 1995. Relative abundance of subunit mRNAs determines gating and Ca(2+) permeability of AMPA receptors in principal neurons and interneurons in rat CNS. Neuron. 15(1), 193–204.","chicago":"Geiger, Jörg, Thorsten Melcher, Duk Koh, Bert Sakmann, Peter Seeburg, Peter M Jonas, and Hannah Monyer. “Relative Abundance of Subunit MRNAs Determines Gating and Ca(2+) Permeability of AMPA Receptors in Principal Neurons and Interneurons in Rat CNS.” <i>Neuron</i>. Elsevier, 1995. <a href=\"https://doi.org/10.1016/0896-6273(95)90076-4\">https://doi.org/10.1016/0896-6273(95)90076-4</a>."},"date_created":"2018-12-11T12:03:33Z","month":"07","page":"193 - 204","status":"public","external_id":{"pmid":["7619522"]},"user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","oa":1,"volume":15,"date_updated":"2022-06-28T07:47:09Z","type":"journal_article","extern":"1","publication_identifier":{"issn":["0896-6273"]},"doi":"10.1016/0896-6273(95)90076-4","author":[{"full_name":"Geiger, Jörg","first_name":"Jörg","last_name":"Geiger"},{"last_name":"Melcher","first_name":"Thorsten","full_name":"Melcher, Thorsten"},{"first_name":"Duk","full_name":"Koh, Duk","last_name":"Koh"},{"last_name":"Sakmann","first_name":"Bert","full_name":"Sakmann, Bert"},{"last_name":"Seeburg","first_name":"Peter","full_name":"Seeburg, Peter"},{"first_name":"Peter M","full_name":"Jonas, Peter M","orcid":"0000-0001-5001-4804","last_name":"Jonas","id":"353C1B58-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Monyer","full_name":"Monyer, Hannah","first_name":"Hannah"}],"_id":"3480","publication":"Neuron","publication_status":"published","abstract":[{"text":"Recording of glutamate-activated currents in membrane patches was combine with RT-PCR-mediated AMPA receptor (AMPAR) subunit mRNA analysis in single identified cells of rat brain slices. Analysis of AMPARs in principal neurons end interneurons of hippocampus and neocortex and in auditory relay neurons and Bergmann glial cells indicates that the GluR-B subunit in its flip version determines formation of receptors with relatively slow gating, whereas the GluR-D subunit promotes assembly of more rapidly gated receptors. The relation between Ca 2+ permeability of AMPAR channels and the relative GluR-B mRNA abundance is consistent with the dominance of this subunit in determining the Ca 2+ permeability of native receptors. The results suggest that differential expression of GluR-B and GluR-D subunit genes, as well as splicing end editing of their mRNAs, account for the differences in gating and Ca 2+ permeability of native AMPAR channels.","lang":"eng"}]}