{"month":"12","article_processing_charge":"No","date_updated":"2023-05-15T13:58:49Z","type":"journal_article","day":"01","abstract":[{"text":"High molecular weight DNA was extracted from the primary Neotropical malaria vector, Anopheles darlingi from Capanema, Pará, Brazil, to create a small insert genomic library, and then a phagemid library. Enriched sublibraries were constructed from the phagemid library using a microsatellite oligo primed second strand synthesis protocol. The resulting 242 760 individual clones were screened. The mean clone size of the positive clones was 302 bp. Flanking primers were designed for each suitable microsatellite sequence. Eight polymorphic loci were optimized and characterized. The allele size ranges are based on 253 samples of A. darlingi from eastern Amazonian and central Brazil.","lang":"eng"}],"date_published":"2001-12-01T00:00:00Z","extern":"1","publication_status":"published","issue":"4","publisher":"Wiley-Blackwell","oa_version":"None","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","date_created":"2018-12-11T12:03:20Z","quality_controlled":"1","title":"Isolation of polymorphic microsatellite markers from the malaria vector Anopheles darlingi","status":"public","publication_identifier":{"issn":["1471-8278"]},"publication":"Molecular Ecology Notes","author":[{"full_name":"Conn, Jan","last_name":"Conn","first_name":"Jan"},{"orcid":"0000-0002-4624-4612","first_name":"Jonathan P","last_name":"Bollback","full_name":"Bollback, Jonathan P","id":"2C6FA9CC-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Onyabe","first_name":"David","full_name":"Onyabe, David"},{"first_name":"Tessa","last_name":"Robinson","full_name":"Robinson, Tessa"},{"full_name":"Wilkerson, Richard","last_name":"Wilkerson","first_name":"Richard"},{"full_name":"Povoa, Marinete","first_name":"Marinete","last_name":"Povoa"}],"page":"223 - 225","doi":" 10.1046/j.1471-8278.2001.00078.x","_id":"3439","language":[{"iso":"eng"}],"article_type":"original","citation":{"ama":"Conn J, Bollback JP, Onyabe D, Robinson T, Wilkerson R, Povoa M. Isolation of polymorphic microsatellite markers from the malaria vector Anopheles darlingi. <i>Molecular Ecology Notes</i>. 2001;1(4):223-225. doi:<a href=\"https://doi.org/ 10.1046/j.1471-8278.2001.00078.x\"> 10.1046/j.1471-8278.2001.00078.x</a>","ista":"Conn J, Bollback JP, Onyabe D, Robinson T, Wilkerson R, Povoa M. 2001. Isolation of polymorphic microsatellite markers from the malaria vector Anopheles darlingi. Molecular Ecology Notes. 1(4), 223–225.","chicago":"Conn, Jan, Jonathan P Bollback, David Onyabe, Tessa Robinson, Richard Wilkerson, and Marinete Povoa. “Isolation of Polymorphic Microsatellite Markers from the Malaria Vector Anopheles Darlingi.” <i>Molecular Ecology Notes</i>. Wiley-Blackwell, 2001. <a href=\"https://doi.org/ 10.1046/j.1471-8278.2001.00078.x\">https://doi.org/ 10.1046/j.1471-8278.2001.00078.x</a>.","apa":"Conn, J., Bollback, J. P., Onyabe, D., Robinson, T., Wilkerson, R., &#38; Povoa, M. (2001). Isolation of polymorphic microsatellite markers from the malaria vector Anopheles darlingi. <i>Molecular Ecology Notes</i>. Wiley-Blackwell. <a href=\"https://doi.org/ 10.1046/j.1471-8278.2001.00078.x\">https://doi.org/ 10.1046/j.1471-8278.2001.00078.x</a>","short":"J. Conn, J.P. Bollback, D. Onyabe, T. Robinson, R. Wilkerson, M. Povoa, Molecular Ecology Notes 1 (2001) 223–225.","mla":"Conn, Jan, et al. “Isolation of Polymorphic Microsatellite Markers from the Malaria Vector Anopheles Darlingi.” <i>Molecular Ecology Notes</i>, vol. 1, no. 4, Wiley-Blackwell, 2001, pp. 223–25, doi:<a href=\"https://doi.org/ 10.1046/j.1471-8278.2001.00078.x\"> 10.1046/j.1471-8278.2001.00078.x</a>.","ieee":"J. Conn, J. P. Bollback, D. Onyabe, T. Robinson, R. Wilkerson, and M. Povoa, “Isolation of polymorphic microsatellite markers from the malaria vector Anopheles darlingi,” <i>Molecular Ecology Notes</i>, vol. 1, no. 4. Wiley-Blackwell, pp. 223–225, 2001."},"year":"2001","intvolume":"         1","publist_id":"2961","volume":1,"acknowledgement":"For  support  in  Brazil  we  thank  D.  Galiza,  R.N.L.  Lacerda,E.P. Santa Rosa, M.N.O. Segura, and R.T.L. de Souza. We also thankM.J.  Braun  for  allowing  work  on  the  library  construction  at  theLaboratory of Molecular Systematics, Washington. Supported byNIH AI 40116 to JEC and Instituto Evandro Chagas, Belém, Brazil."}