{"acknowledgement":"This research was supported by National Institutes of Health Grant GM39697 to R.S.F. D.S. was supported in part by National Institutes of Health training Grant 2T32GM07599. We thank M. A. Krasnow and members of his laboratory, particularly J. Jarecki, for technical guidance, encouragement, and stimulating scientific discussions. We thank A. Maghbouleh and the Stanford Statistics Department Consulting Service for help with statistical analysis. We thank G. Beitel, S. Dietrich, K. Guillemin, D. Micklem, Y. Nakajima, and members of the Fuller and Krasnow laboratories for comments on this manuscript. We thank M. Palazzolo for the use of theDrosophila head cDNA library, D. Kiehart for the use of a Drosophila myosin antibody, and D. Casso, F.-A. Ramirez-Weber, and T. B. Kornberg for use of the D/TM3SbKrGFP flies. We thank A. R. Kidd, D. Tolla, and M. Bender and D. Casso, F.-A. Ramirez-Weber and T. B. Kornberg for communication of results before publication","article_processing_charge":"No","month":"08","date_created":"2018-12-11T12:01:40Z","citation":{"apa":"Siekhaus, D. E., &#38; Fuller, R. (1999). A role for amontillado the Drosophila homolog of the neuropeptide precursor processing protease PC2 in triggering hatching behavior. <i>Journal of Neuroscience</i>. Society for Neuroscience. <a href=\"https://doi.org/10.1523/jneurosci.19-16-06942.1999\">https://doi.org/10.1523/jneurosci.19-16-06942.1999</a>","chicago":"Siekhaus, Daria E, and Robert Fuller. “A Role for Amontillado the Drosophila Homolog of the Neuropeptide Precursor Processing Protease PC2 in Triggering Hatching Behavior.” <i>Journal of Neuroscience</i>. Society for Neuroscience, 1999. <a href=\"https://doi.org/10.1523/jneurosci.19-16-06942.1999\">https://doi.org/10.1523/jneurosci.19-16-06942.1999</a>.","ista":"Siekhaus DE, Fuller R. 1999. A role for amontillado the Drosophila homolog of the neuropeptide precursor processing protease PC2 in triggering hatching behavior. Journal of Neuroscience. 19(16), 6942–6954.","ieee":"D. E. Siekhaus and R. Fuller, “A role for amontillado the Drosophila homolog of the neuropeptide precursor processing protease PC2 in triggering hatching behavior,” <i>Journal of Neuroscience</i>, vol. 19, no. 16. Society for Neuroscience, pp. 6942–6954, 1999.","ama":"Siekhaus DE, Fuller R. A role for amontillado the Drosophila homolog of the neuropeptide precursor processing protease PC2 in triggering hatching behavior. <i>Journal of Neuroscience</i>. 1999;19(16):6942-6954. doi:<a href=\"https://doi.org/10.1523/jneurosci.19-16-06942.1999\">10.1523/jneurosci.19-16-06942.1999</a>","mla":"Siekhaus, Daria E., and Robert Fuller. “A Role for Amontillado the Drosophila Homolog of the Neuropeptide Precursor Processing Protease PC2 in Triggering Hatching Behavior.” <i>Journal of Neuroscience</i>, vol. 19, no. 16, Society for Neuroscience, 1999, pp. 6942–54, doi:<a href=\"https://doi.org/10.1523/jneurosci.19-16-06942.1999\">10.1523/jneurosci.19-16-06942.1999</a>.","short":"D.E. Siekhaus, R. Fuller, Journal of Neuroscience 19 (1999) 6942–6954."},"external_id":{"pmid":["10436051 "]},"status":"public","page":"6942 - 6954","oa":1,"user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","extern":"1","type":"journal_article","date_updated":"2022-09-07T13:48:41Z","volume":19,"publication_identifier":{"issn":["0270-6474"]},"_id":"3148","author":[{"last_name":"Siekhaus","id":"3D224B9E-F248-11E8-B48F-1D18A9856A87","full_name":"Siekhaus, Daria E","first_name":"Daria E","orcid":"0000-0001-8323-8353"},{"last_name":"Fuller","full_name":"Fuller, Robert","first_name":"Robert"}],"doi":"10.1523/jneurosci.19-16-06942.1999","abstract":[{"text":"Accurate proteolytic processing of neuropeptide and peptide hormone precursors by members of the kexin/furin family of proteases is key to determining both the identities and activities of signaling peptides. Here we identify amontillado (amon), the Drosophila melanogaster homolog of the mammalian neuropeptide processing protease PC2, and show that in contrast to vertebrate PC2, amontillado expression undergoes extensive regulation in the nervous system during development. In situ hybridization reveals that expression of amontillado is restricted to the final stages of embryogenesis when it is found in anterior sensory structures and in only 168 cells in the brain and ventral nerve cord. After larvae hatch from their egg shells, the sensory structures and most cells in the CNS turn off or substantially reduce amontillado expression, suggesting that amontillado plays a specific role late in embryogenesis. Larvae lacking the chromosomal region containing amontillado show no gross anatomical defects and respond to touch. However, such larvae show a greatly reduced frequency of a hatching behavior of wild- type Drosophila in which larvae swing their heads, scraping through the eggshell with their mouth hooks. Ubiquitous expression of amontillado can restore near wild-type levels of this behavior, whereas expression of amontillado with an alanine substitution for the catalytic histidine cannot. These results suggest that amontillado expression is regulated as part of a programmed modulation of neural signaling that controls hatching behavior by producing specific neuropeptides in particular neurons at an appropriate developmental time.","lang":"eng"}],"publication_status":"published","publication":"Journal of Neuroscience","pmid":1,"day":"15","year":"1999","article_type":"original","publisher":"Society for Neuroscience","date_published":"1999-08-15T00:00:00Z","publist_id":"3547","language":[{"iso":"eng"}],"intvolume":"        19","scopus_import":"1","quality_controlled":"1","title":"A role for amontillado the Drosophila homolog of the neuropeptide precursor processing protease PC2 in triggering hatching behavior","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6782853/","open_access":"1"}],"oa_version":"Published Version","issue":"16"}