{"editor":[{"last_name":"Suárez","first_name":"María","full_name":"Suárez, María F"},{"full_name":"Bozhkov,  Peter V","first_name":"Peter","last_name":"Bozhkov"}],"_id":"3035","abstract":[{"text":"Embryogenesis of Arabidopsis thaliana follows a nearly invariant cell division pattern and provides an ideal system for studies of early plant development. However, experimental manipulation with embryogenesis is difficult, as the embryo develops deeply inside maternal tissues. Here, we present a method to culture zygotic Arabidopsis embryos in vitro. It enables culturing for prolonged periods of time from the first developmental stages on. The technique omits excision of the embryo by culturing the entire ovule, which facilitates the manual procedure. It allows pharmacological manipulation of embryo development and does not interfere with standard techniques for localizing gene expression and protein localization in the cultivated embryos.","lang":"eng"}],"extern":1,"date_published":"2008-03-07T00:00:00Z","citation":{"apa":"Sauer, M., &#38; Friml, J. (2008). In vitro culture of Arabidopsis embryos . In M. Suárez &#38; P. Bozhkov (Eds.), <i>Plant Embryogenesis</i> (Vol. 427, pp. 71–76). Humana Press. <a href=\"https://doi.org/10.1007/978-1-59745-273-1_5\">https://doi.org/10.1007/978-1-59745-273-1_5</a>","ista":"Sauer M, Friml J. 2008.In vitro culture of Arabidopsis embryos . In: Plant Embryogenesis. Methods In Molecular Biology, vol. 427, 71–76.","chicago":"Sauer, Michael, and Jiří Friml. “In Vitro Culture of Arabidopsis Embryos .” In <i>Plant Embryogenesis</i>, edited by María Suárez and Peter Bozhkov, 427:71–76. Humana Press, 2008. <a href=\"https://doi.org/10.1007/978-1-59745-273-1_5\">https://doi.org/10.1007/978-1-59745-273-1_5</a>.","ama":"Sauer M, Friml J. In vitro culture of Arabidopsis embryos . In: Suárez M, Bozhkov P, eds. <i>Plant Embryogenesis</i>. Vol 427. Humana Press; 2008:71-76. doi:<a href=\"https://doi.org/10.1007/978-1-59745-273-1_5\">10.1007/978-1-59745-273-1_5</a>","short":"M. Sauer, J. Friml, in:, M. Suárez, P. Bozhkov (Eds.), Plant Embryogenesis, Humana Press, 2008, pp. 71–76.","mla":"Sauer, Michael, and Jiří Friml. “In Vitro Culture of Arabidopsis Embryos .” <i>Plant Embryogenesis</i>, edited by María Suárez and Peter Bozhkov, vol. 427, Humana Press, 2008, pp. 71–76, doi:<a href=\"https://doi.org/10.1007/978-1-59745-273-1_5\">10.1007/978-1-59745-273-1_5</a>.","ieee":"M. Sauer and J. Friml, “In vitro culture of Arabidopsis embryos ,” in <i>Plant Embryogenesis</i>, vol. 427, M. Suárez and P. Bozhkov, Eds. Humana Press, 2008, pp. 71–76."},"publication_status":"published","month":"03","date_updated":"2021-01-12T07:40:35Z","type":"book_chapter","page":"71 - 76","doi":"10.1007/978-1-59745-273-1_5","day":"07","publication":"Plant Embryogenesis","author":[{"full_name":"Sauer, Michael","last_name":"Sauer","first_name":"Michael"},{"orcid":"0000-0002-8302-7596","first_name":"Jirí","last_name":"Friml","full_name":"Jirí Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"alternative_title":["Methods In Molecular Biology"],"title":"In vitro culture of Arabidopsis embryos ","quality_controlled":0,"status":"public","publisher":"Humana Press","year":"2008","intvolume":"       427","volume":427,"date_created":"2018-12-11T12:00:59Z","publist_id":"3667"}