{"publisher":"Nature Publishing Group","scopus_import":"1","oa_version":"None","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","date_created":"2018-12-11T12:00:41Z","title":"Auxin transport inhibitors block PIN1 cycling and vesicle trafficking","quality_controlled":"1","status":"public","article_processing_charge":"No","month":"09","pmid":1,"date_updated":"2023-05-16T11:51:44Z","type":"journal_article","day":"27","abstract":[{"text":"Polar transport of the phytohormone auxin mediates various processes in plant growth and development, such as apical dominance, tropisms, vascular patterning and axis formation. This view is based largely on the effects of polar auxin transport inhibitors. These compounds disrupt auxin efflux from the cell but their mode of action is unknown. It is thought that polar auxin flux is caused by the asymmetric distribution of efflux carriers acting at the plasma membrane. The polar localization of efflux carrier candidate PIN1 supports this model. Here we show that the seemingly static localization of PIN1 results from rapid actin-dependent cycling between the plasma membrane and endosomal compartments. Auxin transport inhibitors block PIN1 cycling and inhibit trafficking of membrane proteins that are unrelated to auxin transport. Our data suggest that PIN1 cycling is of central importance for auxin transport and that auxin transport inhibitors affect efflux by generally interfering with membrane-trafficking processes. In support of our conclusion, the vesicle-trafficking inhibitor brefeldin A mimics physiological effects of auxin transport inhibitors.","lang":"eng"}],"publication_status":"published","date_published":"2001-09-27T00:00:00Z","extern":"1","issue":"6854","year":"2001","intvolume":"       413","publist_id":"3719","volume":413,"external_id":{"pmid":["11574889"]},"publication_identifier":{"issn":["0028-0836"]},"doi":"10.1038/35096571","page":"425 - 428","author":[{"first_name":"Niko","last_name":"Geldner","full_name":"Geldner, Niko"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","last_name":"Friml","first_name":"Jirí"},{"first_name":"York","last_name":"Stierhof","full_name":"Stierhof, York"},{"full_name":"Jürgens, Gerd","last_name":"Jürgens","first_name":"Gerd"},{"full_name":"Palme, Klaus","first_name":"Klaus","last_name":"Palme"}],"publication":"Nature","language":[{"iso":"eng"}],"_id":"2983","citation":{"ieee":"N. Geldner, J. Friml, Y. Stierhof, G. Jürgens, and K. Palme, “Auxin transport inhibitors block PIN1 cycling and vesicle trafficking,” <i>Nature</i>, vol. 413, no. 6854. Nature Publishing Group, pp. 425–428, 2001.","mla":"Geldner, Niko, et al. “Auxin Transport Inhibitors Block PIN1 Cycling and Vesicle Trafficking.” <i>Nature</i>, vol. 413, no. 6854, Nature Publishing Group, 2001, pp. 425–28, doi:<a href=\"https://doi.org/10.1038/35096571\">10.1038/35096571</a>.","short":"N. Geldner, J. Friml, Y. Stierhof, G. Jürgens, K. Palme, Nature 413 (2001) 425–428.","apa":"Geldner, N., Friml, J., Stierhof, Y., Jürgens, G., &#38; Palme, K. (2001). Auxin transport inhibitors block PIN1 cycling and vesicle trafficking. <i>Nature</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/35096571\">https://doi.org/10.1038/35096571</a>","ista":"Geldner N, Friml J, Stierhof Y, Jürgens G, Palme K. 2001. Auxin transport inhibitors block PIN1 cycling and vesicle trafficking. Nature. 413(6854), 425–428.","chicago":"Geldner, Niko, Jiří Friml, York Stierhof, Gerd Jürgens, and Klaus Palme. “Auxin Transport Inhibitors Block PIN1 Cycling and Vesicle Trafficking.” <i>Nature</i>. Nature Publishing Group, 2001. <a href=\"https://doi.org/10.1038/35096571\">https://doi.org/10.1038/35096571</a>.","ama":"Geldner N, Friml J, Stierhof Y, Jürgens G, Palme K. Auxin transport inhibitors block PIN1 cycling and vesicle trafficking. <i>Nature</i>. 2001;413(6854):425-428. doi:<a href=\"https://doi.org/10.1038/35096571\">10.1038/35096571</a>"},"article_type":"letter_note"}