{"main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC125484/"}],"abstract":[{"text":"Plants contain a novel unique subfamily of Rho GTPases, vital components of cellular signalling networks. Here we report a general role for some members of this family in polarized plant growth processes. We show that Arabidopsis AtRop4 and AtRop6 encode functional GTPases with similar intrinsic GTP hydrolysis rates. We localized AtRop proteins in root meristem cells to the cross-wall and cell plate membranes. Polar localization of AtRops in trichoblasts specifies the growth sites for emerging root hairs. These sites were visible before budding and elongation of the Arabidopsis root hair when AtRops accumulated at their tips. Expression of constitutively active AtRop4 and AtRop6 mutant proteins in root hairs of transgenic Arabidopsis plants abolished polarized growth and delocalized the tip-focused Ca2+ gradient. Polar localization of AtRops was inhibited by brefeldin A, but not by other drugs such as latrunculin B, cytochalasin D or caffeine. Our results demonstrate a general function of AtRop GTPases in tip growth and in polar diffuse growth.","lang":"eng"}],"issue":"11","date_published":"2001-06-01T00:00:00Z","extern":"1","publication_status":"published","pmid":1,"date_updated":"2023-05-16T12:07:45Z","month":"06","article_processing_charge":"No","day":"01","type":"journal_article","status":"public","quality_controlled":"1","title":"Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth","scopus_import":"1","publisher":"Wiley-Blackwell","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","date_created":"2018-12-11T12:00:40Z","oa":1,"oa_version":"Published Version","language":[{"iso":"eng"}],"_id":"2981","article_type":"original","citation":{"ieee":"A. Molendijk <i>et al.</i>, “Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth,” <i>EMBO Journal</i>, vol. 20, no. 11. Wiley-Blackwell, pp. 2779–2788, 2001.","mla":"Molendijk, Arthur, et al. “Arabidopsis Thaliana Rop GTPases Are Localized to Tips of Root Hairs and Control Polar Growth.” <i>EMBO Journal</i>, vol. 20, no. 11, Wiley-Blackwell, 2001, pp. 2779–88, doi:<a href=\"https://doi.org/10.1093/emboj/20.11.2779\">10.1093/emboj/20.11.2779</a>.","short":"A. Molendijk, F. Bischoff, C. Rajendrakumar, J. Friml, M. Braun, S. Gilroy, K. Palme, EMBO Journal 20 (2001) 2779–2788.","ista":"Molendijk A, Bischoff F, Rajendrakumar C, Friml J, Braun M, Gilroy S, Palme K. 2001. Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth. EMBO Journal. 20(11), 2779–2788.","chicago":"Molendijk, Arthur, Friedrich Bischoff, Chadalavada Rajendrakumar, Jiří Friml, Markus Braun, Simon Gilroy, and Klaus Palme. “Arabidopsis Thaliana Rop GTPases Are Localized to Tips of Root Hairs and Control Polar Growth.” <i>EMBO Journal</i>. Wiley-Blackwell, 2001. <a href=\"https://doi.org/10.1093/emboj/20.11.2779\">https://doi.org/10.1093/emboj/20.11.2779</a>.","apa":"Molendijk, A., Bischoff, F., Rajendrakumar, C., Friml, J., Braun, M., Gilroy, S., &#38; Palme, K. (2001). Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth. <i>EMBO Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1093/emboj/20.11.2779\">https://doi.org/10.1093/emboj/20.11.2779</a>","ama":"Molendijk A, Bischoff F, Rajendrakumar C, et al. Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth. <i>EMBO Journal</i>. 2001;20(11):2779-2788. doi:<a href=\"https://doi.org/10.1093/emboj/20.11.2779\">10.1093/emboj/20.11.2779</a>"},"publication_identifier":{"issn":["0261-4189"]},"external_id":{"pmid":["11387211"]},"doi":"10.1093/emboj/20.11.2779","page":"2779 - 2788","author":[{"first_name":"Arthur","last_name":"Molendijk","full_name":"Molendijk, Arthur"},{"first_name":"Friedrich","last_name":"Bischoff","full_name":"Bischoff, Friedrich"},{"full_name":"Rajendrakumar, Chadalavada","last_name":"Rajendrakumar","first_name":"Chadalavada"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","last_name":"Friml","first_name":"Jirí"},{"last_name":"Braun","first_name":"Markus","full_name":"Braun, Markus"},{"full_name":"Gilroy, Simon","first_name":"Simon","last_name":"Gilroy"},{"full_name":"Palme, Klaus","last_name":"Palme","first_name":"Klaus"}],"publication":"EMBO Journal","acknowledgement":"We thank Drs Frantisek Baluška, Matthias Godde, Peter Huijser, Lars Vahlkamp and Dieter Volkmann for help, criticism and constructive reading of the manuscript. We are grateful to Dr N.-H.Chua for providing us with pTA7002. The work was funded by the DFG, the European Communities Biotechnology Programme (Bio4-CT98 0239) and the INCO Copernicus Programme (IC15-CT96-0920). C.S.V.R. is the recipient of an Alexander von Humboldt fellowship and J.F. of a DAAD fellowship.","year":"2001","publist_id":"3721","volume":20,"intvolume":"        20"}