{"department":[{"_id":"JoCs"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","doi":"10.1016/j.neuron.2013.01.021","_id":"2845","month":"03","publisher":"Elsevier","issue":"6","scopus_import":1,"author":[{"first_name":"Tobias","full_name":"Rose, Tobias","last_name":"Rose"},{"last_name":"Schönenberger","first_name":"Philipp","full_name":"Schönenberger, Philipp","id":"3B9D816C-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Jezek, Karel","first_name":"Karel","last_name":"Jezek"},{"last_name":"Oertner","first_name":"Thomas","full_name":"Oertner, Thomas"}],"intvolume":"        77","page":"1109 - 1121","language":[{"iso":"eng"}],"volume":77,"oa_version":"None","date_updated":"2021-01-12T07:00:11Z","title":"Developmental refinement of vesicle cycling at Schaffer collateral synapses","publist_id":"3949","abstract":[{"text":"At synapses formed between dissociated neurons, about half of all synaptic vesicles are refractory to evoked release, forming the so-called &quot;resting pool.&quot; Here, we use optical measurements of vesicular pH to study developmental changes in pool partitioning and vesicle cycling in cultured hippocampal slices. Two-photon imaging of a genetically encoded two-color release sensor (ratio-sypHy) allowed us to perform calibrated measurements at individual Schaffer collateral boutons. Mature boutons released a large fraction of their vesicles during simulated place field activity, and vesicle retrieval rates were 7-fold higher compared to immature boutons. Saturating stimulation mobilized essentially all vesicles at mature synapses. Resting pool formation and a concomitant reduction in evoked release was induced by chronic depolarization but not by acute inhibition of the protein phosphatase calcineurin. We conclude that synapses in CA1 undergo a prominent refinement of vesicle use during early postnatal development that is not recapitulated in dissociated neuronal culture.","lang":"eng"}],"quality_controlled":"1","year":"2013","publication_status":"published","date_published":"2013-03-20T00:00:00Z","day":"20","citation":{"ama":"Rose T, Schönenberger P, Jezek K, Oertner T. Developmental refinement of vesicle cycling at Schaffer collateral synapses. <i>Neuron</i>. 2013;77(6):1109-1121. doi:<a href=\"https://doi.org/10.1016/j.neuron.2013.01.021\">10.1016/j.neuron.2013.01.021</a>","short":"T. Rose, P. Schönenberger, K. Jezek, T. Oertner, Neuron 77 (2013) 1109–1121.","chicago":"Rose, Tobias, Philipp Schönenberger, Karel Jezek, and Thomas Oertner. “Developmental Refinement of Vesicle Cycling at Schaffer Collateral Synapses.” <i>Neuron</i>. Elsevier, 2013. <a href=\"https://doi.org/10.1016/j.neuron.2013.01.021\">https://doi.org/10.1016/j.neuron.2013.01.021</a>.","apa":"Rose, T., Schönenberger, P., Jezek, K., &#38; Oertner, T. (2013). Developmental refinement of vesicle cycling at Schaffer collateral synapses. <i>Neuron</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neuron.2013.01.021\">https://doi.org/10.1016/j.neuron.2013.01.021</a>","ista":"Rose T, Schönenberger P, Jezek K, Oertner T. 2013. Developmental refinement of vesicle cycling at Schaffer collateral synapses. Neuron. 77(6), 1109–1121.","ieee":"T. Rose, P. Schönenberger, K. Jezek, and T. Oertner, “Developmental refinement of vesicle cycling at Schaffer collateral synapses,” <i>Neuron</i>, vol. 77, no. 6. Elsevier, pp. 1109–1121, 2013.","mla":"Rose, Tobias, et al. “Developmental Refinement of Vesicle Cycling at Schaffer Collateral Synapses.” <i>Neuron</i>, vol. 77, no. 6, Elsevier, 2013, pp. 1109–21, doi:<a href=\"https://doi.org/10.1016/j.neuron.2013.01.021\">10.1016/j.neuron.2013.01.021</a>."},"status":"public","date_created":"2018-12-11T11:59:54Z","type":"journal_article","publication":"Neuron"}