{"abstract":[{"text":"Glutamate and GABA are the main transmitters in the central nervous system and their effects are mediated by ionotropic and metabotropic receptors. Immunogold electron microscopy has revealed the quantitative localization of these receptors at 20-30 nm resolution. SDS-digested freeze-fracture replica labeling (SDS-FRL), a newly developed immunogold method, provides an accurate estimate of molecule numbers. Here, we summarize the recent advances in quantitative receptor localization, including use of SDS-FRL analyses to determine numbers of AMPA-type glutamate receptors in the cerebellum. The two-dimensional view and high sensitivity of SDS-FRL have revealed small, irregularly shaped AMPA receptor clusters within cerebellar synapses.","lang":"eng"}],"_id":"2670","issue":"3","citation":{"short":"M. Masugi Tokita, R. Shigemoto, Current Opinion in Neurobiology 17 (2007) 387–393.","ama":"Masugi Tokita M, Shigemoto R. High-resolution quantitative visualization of glutamate and GABA receptors at central synapses. <i>Current Opinion in Neurobiology</i>. 2007;17(3):387-393. doi:<a href=\"https://doi.org/10.1016/j.conb.2007.04.012\">10.1016/j.conb.2007.04.012</a>","apa":"Masugi Tokita, M., &#38; Shigemoto, R. (2007). High-resolution quantitative visualization of glutamate and GABA receptors at central synapses. <i>Current Opinion in Neurobiology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.conb.2007.04.012\">https://doi.org/10.1016/j.conb.2007.04.012</a>","ista":"Masugi Tokita M, Shigemoto R. 2007. High-resolution quantitative visualization of glutamate and GABA receptors at central synapses. Current Opinion in Neurobiology. 17(3), 387–393.","chicago":"Masugi Tokita, Miwako, and Ryuichi Shigemoto. “High-Resolution Quantitative Visualization of Glutamate and GABA Receptors at Central Synapses.” <i>Current Opinion in Neurobiology</i>. Elsevier, 2007. <a href=\"https://doi.org/10.1016/j.conb.2007.04.012\">https://doi.org/10.1016/j.conb.2007.04.012</a>.","ieee":"M. Masugi Tokita and R. Shigemoto, “High-resolution quantitative visualization of glutamate and GABA receptors at central synapses,” <i>Current Opinion in Neurobiology</i>, vol. 17, no. 3. Elsevier, pp. 387–393, 2007.","mla":"Masugi Tokita, Miwako, and Ryuichi Shigemoto. “High-Resolution Quantitative Visualization of Glutamate and GABA Receptors at Central Synapses.” <i>Current Opinion in Neurobiology</i>, vol. 17, no. 3, Elsevier, 2007, pp. 387–93, doi:<a href=\"https://doi.org/10.1016/j.conb.2007.04.012\">10.1016/j.conb.2007.04.012</a>."},"date_published":"2007-06-01T00:00:00Z","publication_status":"published","extern":1,"date_updated":"2020-07-14T12:45:44Z","month":"06","day":"01","publication":"Current Opinion in Neurobiology","author":[{"full_name":"Masugi-Tokita, Miwako","last_name":"Masugi Tokita","first_name":"Miwako"},{"id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","full_name":"Ryuichi Shigemoto","last_name":"Shigemoto","first_name":"Ryuichi","orcid":"0000-0001-8761-9444"}],"doi":"10.1016/j.conb.2007.04.012","page":"387 - 393","type":"review","status":"public","quality_controlled":0,"title":"High-resolution quantitative visualization of glutamate and GABA receptors at central synapses","year":"2007","publisher":"Elsevier","publist_id":"4226","volume":17,"date_created":"2018-12-11T11:58:58Z","intvolume":"        17"}