{"article_type":"original","doi":"10.1074/jbc.M207531200","title":"Subtype-specific expression of Group III metabotropic glutamate receptors and Ca2+ channels in single nerve terminals","external_id":{"pmid":["12376542"]},"article_processing_charge":"No","author":[{"first_name":"Carmelo","full_name":"Millán, Carmelo","last_name":"Millán"},{"full_name":"Luján, Rafael","last_name":"Luján","first_name":"Rafael"},{"last_name":"Shigemoto","full_name":"Shigemoto, Ryuichi","orcid":"0000-0001-8761-9444","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","first_name":"Ryuichi"},{"last_name":"Sánchez Prieto","full_name":"Sánchez Prieto, José","first_name":"José"}],"pmid":1,"intvolume":"       277","publisher":"American Society for Biochemistry and Molecular Biology","publication_identifier":{"issn":["0021-9258"]},"abstract":[{"text":"The release properties of glutamatergic nerve terminals are influenced by a number of factors, including the subtype of voltage-dependent calcium channel and the presence of presynaptic autoreceptors. Group III metabotropic glutamate receptors (mGluRs) mediate feedback inhibition of glutamate release by inhibiting Ca2+ channel activity. By imaging Ca2+ in preparations of cerebrocortical nerve terminals, we show that voltage-dependent Ca2+ channels are distributed in a heterogeneous manner in individual nerve terminals. Presynaptic terminals contained only N-type (47.5%; conotoxin GVIA-sensitive), P/Q-type (3.9%; agatoxin IVA-sensitive), or both N- and P/Q-type (42.6%) Ca2+ channels, although the remainder of the terminals (6.1%) were insensitive to these two toxins. In this preparation, two mGluRs with high and low affinity for L(+)-2-amino-4-phosphonobutyrate were identified by immunocytochemistry as mGluR4 and mGluR7, respectively. These receptors were responsible for 22.2 and 24.1% reduction of glutamate release, and they reduced the Ca2+ response in 24.4 and 30.3% of the nerve terminals, respectively. Interestingly, mGluR4 was largely (73.7%) located in nerve terminals expressing both N- and P/Q-type Ca2+ channels, whereas mGluR7 was predominantly (69.9%) located in N-type Ca2+ channel-expressing terminals. This specific coexpression of different group III mGluRs and Ca2+ channels may endow synaptic terminals with distinct release properties and reveals the existence of a high degree of presynaptic heterogeneity.","lang":"eng"}],"date_created":"2018-12-11T11:58:43Z","volume":277,"citation":{"ista":"Millán C, Luján R, Shigemoto R, Sánchez Prieto J. 2002. Subtype-specific expression of Group III metabotropic glutamate receptors and Ca2+ channels in single nerve terminals. Journal of Biological Chemistry. 277(49), 47796–47803.","ama":"Millán C, Luján R, Shigemoto R, Sánchez Prieto J. Subtype-specific expression of Group III metabotropic glutamate receptors and Ca2+ channels in single nerve terminals. <i>Journal of Biological Chemistry</i>. 2002;277(49):47796-47803. doi:<a href=\"https://doi.org/10.1074/jbc.M207531200\">10.1074/jbc.M207531200</a>","short":"C. Millán, R. Luján, R. Shigemoto, J. Sánchez Prieto, Journal of Biological Chemistry 277 (2002) 47796–47803.","mla":"Millán, Carmelo, et al. “Subtype-Specific Expression of Group III Metabotropic Glutamate Receptors and Ca2+ Channels in Single Nerve Terminals.” <i>Journal of Biological Chemistry</i>, vol. 277, no. 49, American Society for Biochemistry and Molecular Biology, 2002, pp. 47796–803, doi:<a href=\"https://doi.org/10.1074/jbc.M207531200\">10.1074/jbc.M207531200</a>.","ieee":"C. Millán, R. Luján, R. Shigemoto, and J. Sánchez Prieto, “Subtype-specific expression of Group III metabotropic glutamate receptors and Ca2+ channels in single nerve terminals,” <i>Journal of Biological Chemistry</i>, vol. 277, no. 49. American Society for Biochemistry and Molecular Biology, pp. 47796–47803, 2002.","chicago":"Millán, Carmelo, Rafael Luján, Ryuichi Shigemoto, and José Sánchez Prieto. “Subtype-Specific Expression of Group III Metabotropic Glutamate Receptors and Ca2+ Channels in Single Nerve Terminals.” <i>Journal of Biological Chemistry</i>. American Society for Biochemistry and Molecular Biology, 2002. <a href=\"https://doi.org/10.1074/jbc.M207531200\">https://doi.org/10.1074/jbc.M207531200</a>.","apa":"Millán, C., Luján, R., Shigemoto, R., &#38; Sánchez Prieto, J. (2002). Subtype-specific expression of Group III metabotropic glutamate receptors and Ca2+ channels in single nerve terminals. <i>Journal of Biological Chemistry</i>. American Society for Biochemistry and Molecular Biology. <a href=\"https://doi.org/10.1074/jbc.M207531200\">https://doi.org/10.1074/jbc.M207531200</a>"},"date_published":"2002-12-02T00:00:00Z","type":"journal_article","publication":"Journal of Biological Chemistry","year":"2002","acknowledgement":"We thank M. Sefton for editorial assistance.","status":"public","issue":"49","scopus_import":"1","oa_version":"Published Version","language":[{"iso":"eng"}],"quality_controlled":"1","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","day":"02","month":"12","page":"47796 - 47803","_id":"2621","publist_id":"4277","extern":"1","publication_status":"published","date_updated":"2023-07-19T07:49:19Z"}