{"day":"01","type":"journal_article","pmid":1,"date_updated":"2023-05-24T09:34:13Z","article_processing_charge":"No","month":"06","issue":"6","publication_status":"published","date_published":"2001-06-01T00:00:00Z","extern":"1","abstract":[{"lang":"eng","text":"Alternative splicing in the mGluR5 gene generates two different receptor isoforms, of which expression is developmentally regulated. However, little is known about the functional significance of mGluR5 splice variants. We have examined the functional coupling, subcellular targeting, and effect on neuronal differentiation of epitope-tagged mGluR5 isoforms by expression in neuroblastoma NG108-15 cells. We found that both mGluR5 splice variants give rise to comparable [Ca2+]i transients and have similar pharmacological profile. Tagged receptors were shown by immunofluorescence to be inserted in the plasma membrane. In undifferentiated cells the subcellular localization of the two mGluR5 isoforms was partially segregated, whereas in differentiated cells the labeling largely redistributed to the newly formed neurites. Interestingly, we demonstrate that mGluR5 splice variants dramatically influence the formation and maturation of neurites; mGluR5a hinders the acquisition of mature neuronal traits and mGluR5b fosters the elaboration and extension of neurites. These effects are partly inhibited by MPEP."}],"user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","date_created":"2018-12-11T11:58:38Z","oa_version":"None","scopus_import":"1","publisher":"Academic Press","status":"public","quality_controlled":"1","title":"Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms","page":"957 - 972","doi":"10.1006/mcne.2001.0993","author":[{"first_name":"Silvia","last_name":"Mion","full_name":"Mion, Silvia"},{"last_name":"Corti","first_name":"Corrado","full_name":"Corti, Corrado"},{"last_name":"Neki","first_name":"Akio","full_name":"Neki, Akio"},{"last_name":"Shigemoto","first_name":"Ryuichi","full_name":"Shigemoto, Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8761-9444"},{"last_name":"Corsi","first_name":"Mauro","full_name":"Corsi, Mauro"},{"first_name":"Guido","last_name":"Fumagalli","full_name":"Fumagalli, Guido"},{"full_name":"Ferraguti, Francesco","first_name":"Francesco","last_name":"Ferraguti"}],"publication":"Molecular and Cellular Neuroscience","publication_identifier":{"issn":["1044-7431"]},"external_id":{"pmid":["11414786"]},"citation":{"mla":"Mion, Silvia, et al. “Bidirectional Regulation of Neurite Elaboration by Alternatively Spliced Metabotropic Glutamate Receptor 5 (MGluR5) Isoforms.” <i>Molecular and Cellular Neuroscience</i>, vol. 17, no. 6, Academic Press, 2001, pp. 957–72, doi:<a href=\"https://doi.org/10.1006/mcne.2001.0993\">10.1006/mcne.2001.0993</a>.","ieee":"S. Mion <i>et al.</i>, “Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms,” <i>Molecular and Cellular Neuroscience</i>, vol. 17, no. 6. Academic Press, pp. 957–972, 2001.","ama":"Mion S, Corti C, Neki A, et al. Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms. <i>Molecular and Cellular Neuroscience</i>. 2001;17(6):957-972. doi:<a href=\"https://doi.org/10.1006/mcne.2001.0993\">10.1006/mcne.2001.0993</a>","apa":"Mion, S., Corti, C., Neki, A., Shigemoto, R., Corsi, M., Fumagalli, G., &#38; Ferraguti, F. (2001). Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms. <i>Molecular and Cellular Neuroscience</i>. Academic Press. <a href=\"https://doi.org/10.1006/mcne.2001.0993\">https://doi.org/10.1006/mcne.2001.0993</a>","ista":"Mion S, Corti C, Neki A, Shigemoto R, Corsi M, Fumagalli G, Ferraguti F. 2001. Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms. Molecular and Cellular Neuroscience. 17(6), 957–972.","chicago":"Mion, Silvia, Corrado Corti, Akio Neki, Ryuichi Shigemoto, Mauro Corsi, Guido Fumagalli, and Francesco Ferraguti. “Bidirectional Regulation of Neurite Elaboration by Alternatively Spliced Metabotropic Glutamate Receptor 5 (MGluR5) Isoforms.” <i>Molecular and Cellular Neuroscience</i>. Academic Press, 2001. <a href=\"https://doi.org/10.1006/mcne.2001.0993\">https://doi.org/10.1006/mcne.2001.0993</a>.","short":"S. Mion, C. Corti, A. Neki, R. Shigemoto, M. Corsi, G. Fumagalli, F. Ferraguti, Molecular and Cellular Neuroscience 17 (2001) 957–972."},"article_type":"original","language":[{"iso":"eng"}],"_id":"2607","publist_id":"4291","volume":17,"intvolume":"        17","year":"2001","acknowledgement":"The authors thank: Dr. J. M. Rimland and M. T. Scupoli for their technical help with X. oocytes recordings and FAC sorting, respectively; Dr. Y. Dalezios for helping with the statistical analyses; and Dr. G. Varani for helping with the analyses of mRNA and genomic sequences. We are also grateful to Professor F. Benfenati, Dr. F. Conquet, Dr. Rafael Lujan, Dr. J. McIlhinney, Professor P. Somogyi, Dr. J. H. Xuereb, and Dr. M. Zoli for careful reading of the manuscript\r\nand helpful suggestions. R.S. is supported by the Laboratory of Cerebral Structure, National Institute for Physiological Sciences, Myodaiji, Okazaki 444-8585, CREST Japan Science and Technology Corporation, Japan."}