{"acknowledgement":"We thank Drs. S. Nakanishi, K. Moriyoshi, V. I. Gelfand, and P. J. Conn for gifts of cDNAs and antibodies, A. S. Serpinskaya and H. Wu for excellent preparation of neuron cultures, and H. J. Chung for help in immunoblots. This work was supported by the Pew Chari-table Trust and National Institutes of Health grants NS33184 and NS39286 (A. M. C.), K02MH01152 and NIDA DA10309 (P. W.), and a fellowship from IPSEN Foundation (H. B.). ","volume":28,"publist_id":"4295","intvolume":"        28","year":"2000","article_type":"original","citation":{"short":"H. Boudin, A. Doan, J. Xia, R. Shigemoto, R. Huganir, P. Worley, A. Craig, Neuron 28 (2000) 485–497.","chicago":"Boudin, Hélène, Andrew Doan, Jun Xia, Ryuichi Shigemoto, Richard Huganir, Paul Worley, and Ann Craig. “Presynaptic Clustering of MGluR7a Requires the PICK1 PDZ Domain Binding Site.” <i>Neuron</i>. Elsevier, 2000. <a href=\"https://doi.org/10.1016/S0896-6273(00)00127-6\">https://doi.org/10.1016/S0896-6273(00)00127-6</a>.","apa":"Boudin, H., Doan, A., Xia, J., Shigemoto, R., Huganir, R., Worley, P., &#38; Craig, A. (2000). Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain binding site. <i>Neuron</i>. Elsevier. <a href=\"https://doi.org/10.1016/S0896-6273(00)00127-6\">https://doi.org/10.1016/S0896-6273(00)00127-6</a>","ista":"Boudin H, Doan A, Xia J, Shigemoto R, Huganir R, Worley P, Craig A. 2000. Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain binding site. Neuron. 28(2), 485–497.","ama":"Boudin H, Doan A, Xia J, et al. Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain binding site. <i>Neuron</i>. 2000;28(2):485-497. doi:<a href=\"https://doi.org/10.1016/S0896-6273(00)00127-6\">10.1016/S0896-6273(00)00127-6</a>","ieee":"H. Boudin <i>et al.</i>, “Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain binding site,” <i>Neuron</i>, vol. 28, no. 2. Elsevier, pp. 485–497, 2000.","mla":"Boudin, Hélène, et al. “Presynaptic Clustering of MGluR7a Requires the PICK1 PDZ Domain Binding Site.” <i>Neuron</i>, vol. 28, no. 2, Elsevier, 2000, pp. 485–97, doi:<a href=\"https://doi.org/10.1016/S0896-6273(00)00127-6\">10.1016/S0896-6273(00)00127-6</a>."},"_id":"2603","language":[{"iso":"eng"}],"publication":"Neuron","author":[{"full_name":"Boudin, Hélène","first_name":"Hélène","last_name":"Boudin"},{"full_name":"Doan, Andrew","first_name":"Andrew","last_name":"Doan"},{"last_name":"Xia","first_name":"Jun","full_name":"Xia, Jun"},{"orcid":"0000-0001-8761-9444","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","full_name":"Shigemoto, Ryuichi","last_name":"Shigemoto","first_name":"Ryuichi"},{"first_name":"Richard","last_name":"Huganir","full_name":"Huganir, Richard"},{"full_name":"Worley, Paul","last_name":"Worley","first_name":"Paul"},{"last_name":"Craig","first_name":"Ann","full_name":"Craig, Ann"}],"page":"485 - 497","doi":"10.1016/S0896-6273(00)00127-6","publication_identifier":{"issn":["0896-6273"]},"external_id":{"pmid":["11144358"]},"status":"public","title":"Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain binding site","quality_controlled":"1","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","date_created":"2018-12-11T11:58:37Z","oa_version":"None","scopus_import":"1","publisher":"Elsevier","issue":"2","date_published":"2000-11-01T00:00:00Z","publication_status":"published","extern":"1","abstract":[{"text":"Aggregation of neurotransmitter receptors at pre- and postsynaptic structures is crucial for efficient neuronal communication. In contrast to the wealth of information about postsynaptic specializations, little is known about the molecular organization of presynaptic membrane proteins. We show here that the metabotropic glutamate receptor mGluR7a, which localizes specifically to presynaptic active zones, interacts in vitro and in vivo with PICK1. Coexpression in heterologous systems induces coclustering dependent upon the extreme C terminus of mGluR7a and the PDZ domain of PICK1. mGluR7a and PICK1 localize to excitatory synapses in hippocampal neurons. Furthermore, whereas transfected mGluR7a clusters at presynaptic sites, mGluR7aΔ3 lacking the PICK1 binding site targets to axons but does not cluster. These results suggest that PICK1 is a component of the presynaptic machinery involved in mGlUR7a aggregation and in modulation of glutamate neurotransmission.","lang":"eng"}],"day":"01","type":"journal_article","pmid":1,"date_updated":"2023-05-03T09:41:55Z","article_processing_charge":"No","month":"11"}