{"date_updated":"2022-09-13T08:30:25Z","pmid":1,"article_processing_charge":"No","month":"09","day":"13","type":"journal_article","abstract":[{"lang":"eng","text":"A γ-aminobutyric acid (GABA)(B) receptor (named GABA(B)R1) has been recently cloned in the rat and human brain and two variants generated by alternative RNA splicing were identified. In the present study, we addressed the question as to whether these variants contribute to the diversity of GABA(B) receptor-mediated physiological responses and constitute real receptor subtypes with distinct functions. To this aim, we have mapped the GABA(B)R1 (R1a) and GABA(B)R1b (R1b) transcript distribution in the rat brain using in situ hybridization. We have compared the mRNA distribution with the distribution of [ 3H]CGP54626-labeled binding GABA(B)R1 receptor sites as assessed in adjacent cryosections by quantitative autoradiography. We found that GABA(B) receptor transcripts and binding sites are expressed in the brain in almost all neuronal cell populations. Expression in glial cells, if any, is marginal. We observed a good parallelism between GABA(B)R1 mRNA transcripts and binding sites in broad neuroanatomical entities with highest densities in hippocampus, thalamic nuclei, and cerebellum. By contrast, R1a and R1b transcripts exhibit marked differences in their regional and cellular distribution pattern. A typical example is the cerebellum with an almost exclusive expression of R1b in the Purkinje cells and of R1a in the granule, stellate, and basket cells. Data pointing at a pre- versus postsynaptic localization for R1a and R1b, respectively, at some neuronal sites are presented.\r\n"}],"issue":"1","date_published":"1999-09-13T00:00:00Z","extern":"1","publication_status":"published","scopus_import":"1","publisher":"Wiley-Blackwell","date_created":"2018-12-11T11:58:35Z","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","oa_version":"None","status":"public","title":"Spatial distribution of GABA(B)R1 receptor mRNA and binding sites in the rat brain^","quality_controlled":"1","publication_identifier":{"issn":["0021-9967"]},"external_id":{"pmid":["10440706 "]},"page":"1 - 16","doi":"10.1002/(SICI)1096-9861(19990913)412:1<1::AID-CNE1>3.0.CO;2-D","author":[{"first_name":"Serge","last_name":"Bischoff","full_name":"Bischoff, Serge"},{"full_name":"Leonhard, Sabine","last_name":"Leonhard","first_name":"Sabine"},{"full_name":"Reymann, Nicole","last_name":"Reymann","first_name":"Nicole"},{"last_name":"Schuler","first_name":"Valérie","full_name":"Schuler, Valérie"},{"id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","full_name":"Shigemoto, Ryuichi","first_name":"Ryuichi","last_name":"Shigemoto","orcid":"0000-0001-8761-9444"},{"full_name":"Kaupmann, Klemens","last_name":"Kaupmann","first_name":"Klemens"},{"last_name":"Bettler","first_name":"Bernhard","full_name":"Bettler, Bernhard"}],"publication":"Journal of Comparative Neurology","language":[{"iso":"eng"}],"_id":"2596","article_type":"original","citation":{"ieee":"S. Bischoff et al., “Spatial distribution of GABA(B)R1 receptor mRNA and binding sites in the rat brain^,” Journal of Comparative Neurology, vol. 412, no. 1. Wiley-Blackwell, pp. 1–16, 1999.","mla":"Bischoff, Serge, et al. “Spatial Distribution of GABA(B)R1 Receptor MRNA and Binding Sites in the Rat Brain^.” Journal of Comparative Neurology, vol. 412, no. 1, Wiley-Blackwell, 1999, pp. 1–16, doi:10.1002/(SICI)1096-9861(19990913)412:1<1::AID-CNE1>3.0.CO;2-D.","short":"S. Bischoff, S. Leonhard, N. Reymann, V. Schuler, R. Shigemoto, K. Kaupmann, B. Bettler, Journal of Comparative Neurology 412 (1999) 1–16.","chicago":"Bischoff, Serge, Sabine Leonhard, Nicole Reymann, Valérie Schuler, Ryuichi Shigemoto, Klemens Kaupmann, and Bernhard Bettler. “Spatial Distribution of GABA(B)R1 Receptor MRNA and Binding Sites in the Rat Brain^.” Journal of Comparative Neurology. Wiley-Blackwell, 1999. https://doi.org/10.1002/(SICI)1096-9861(19990913)412:1<1::AID-CNE1>3.0.CO;2-D.","ista":"Bischoff S, Leonhard S, Reymann N, Schuler V, Shigemoto R, Kaupmann K, Bettler B. 1999. Spatial distribution of GABA(B)R1 receptor mRNA and binding sites in the rat brain^. Journal of Comparative Neurology. 412(1), 1–16.","apa":"Bischoff, S., Leonhard, S., Reymann, N., Schuler, V., Shigemoto, R., Kaupmann, K., & Bettler, B. (1999). Spatial distribution of GABA(B)R1 receptor mRNA and binding sites in the rat brain^. Journal of Comparative Neurology. Wiley-Blackwell. https://doi.org/10.1002/(SICI)1096-9861(19990913)412:1<1::AID-CNE1>3.0.CO;2-D","ama":"Bischoff S, Leonhard S, Reymann N, et al. Spatial distribution of GABA(B)R1 receptor mRNA and binding sites in the rat brain^. Journal of Comparative Neurology. 1999;412(1):1-16. doi:10.1002/(SICI)1096-9861(19990913)412:1<1::AID-CNE1>3.0.CO;2-D"},"year":"1999","publist_id":"4303","volume":412,"intvolume":" 412","acknowledgement":"We thank W. Froestl and S.J. Mickel for synthesis of GABAB ligands, and H. van der Putten for helpful discussions. Many thanks to S. Pfister (former graduate student in our group), and P. Dreja, M. Kohler, P. Schwarb, P.Kaindl (Carl Zeiss A.G., Zurich, Switzerland) for their help in the design of computer macros for the quantitative analysis of in situ hybridization sections. The critical review of the manuscript by J. Mosbacher (NovartisPharma, TA Nervous System) and J. Koenig (Maryland Psychiatric Research Center, Baltimore, MD) are gratefully acknowledged."}