{"main_file_link":[{"url":"http://genesdev.cshlp.org/content/6/12b/2620","open_access":"1"}],"abstract":[{"text":"We report the molecular characterization of two novel rat helix-loop-helix (HLH) proteins, designated HES-1 and HES-3, that show structural homology to the Drosophila hairy and Enhancer of split [E(spl)] proteins, both of which are required for normal neurogenesis. HES-1 mRNA, expressed in various tissues of both embryos and adults, is present at a high level in the epithelial cells, including the embryonal neuroepithelial cells, as well as in the mesoderm-derived tissues such as the embryonal muscle. In contrast, HES-3 mRNA is produced exclusively in cerebellar Purkinje cells. HES-1 represses transcription by binding to the N box, which is a recognition sequence of E(spl) proteins. Interestingly, neither HES-1 nor HES-3 alone interacts efficiently with the E box, but each protein decreases the transcription induced by E-box-binding HLH activators such as E47. Furthermore, HES-1 also inhibits the functions of MyoD and MASH1 and effectively diminishes the myogenic conversion of C3H10T1/2 cells induced by MyoD. These results suggest that HES-1 may play an important role in mammalian development by negatively acting on the two different sequences while HES-3 acts as a repressor in a specific type of neurons.","lang":"eng"}],"publication_status":"published","extern":"1","date_published":"1992-01-01T00:00:00Z","issue":"12 B","month":"01","article_processing_charge":"No","date_updated":"2022-03-17T14:52:29Z","pmid":1,"type":"journal_article","day":"01","quality_controlled":"1","title":"Two mammalian helix-loop-helix factors structurally related to Drosophila hairy and Enhancer of split","status":"public","publisher":"Cold Spring Harbor Laboratory Press","scopus_import":"1","oa":1,"oa_version":"Published Version","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","date_created":"2018-12-11T11:58:15Z","language":[{"iso":"eng"}],"_id":"2535","article_type":"original","citation":{"mla":"Sasai, Yoshiki, et al. “Two Mammalian Helix-Loop-Helix Factors Structurally Related to Drosophila Hairy and Enhancer of Split.” <i>Genes and Development</i>, vol. 6, no. 12 B, Cold Spring Harbor Laboratory Press, 1992, pp. 2620–34, doi:<a href=\"https://doi.org/10.1101/gad.6.12b.2620\">10.1101/gad.6.12b.2620</a>.","ieee":"Y. Sasai, R. Kageyama, Y. Tagawa, R. Shigemoto, and S. Nakanishi, “Two mammalian helix-loop-helix factors structurally related to Drosophila hairy and Enhancer of split,” <i>Genes and Development</i>, vol. 6, no. 12 B. Cold Spring Harbor Laboratory Press, pp. 2620–2634, 1992.","apa":"Sasai, Y., Kageyama, R., Tagawa, Y., Shigemoto, R., &#38; Nakanishi, S. (1992). Two mammalian helix-loop-helix factors structurally related to Drosophila hairy and Enhancer of split. <i>Genes and Development</i>. Cold Spring Harbor Laboratory Press. <a href=\"https://doi.org/10.1101/gad.6.12b.2620\">https://doi.org/10.1101/gad.6.12b.2620</a>","chicago":"Sasai, Yoshiki, Ryoichiro Kageyama, Yoshiaki Tagawa, Ryuichi Shigemoto, and Shigetada Nakanishi. “Two Mammalian Helix-Loop-Helix Factors Structurally Related to Drosophila Hairy and Enhancer of Split.” <i>Genes and Development</i>. Cold Spring Harbor Laboratory Press, 1992. <a href=\"https://doi.org/10.1101/gad.6.12b.2620\">https://doi.org/10.1101/gad.6.12b.2620</a>.","ista":"Sasai Y, Kageyama R, Tagawa Y, Shigemoto R, Nakanishi S. 1992. Two mammalian helix-loop-helix factors structurally related to Drosophila hairy and Enhancer of split. Genes and Development. 6(12 B), 2620–2634.","ama":"Sasai Y, Kageyama R, Tagawa Y, Shigemoto R, Nakanishi S. Two mammalian helix-loop-helix factors structurally related to Drosophila hairy and Enhancer of split. <i>Genes and Development</i>. 1992;6(12 B):2620-2634. doi:<a href=\"https://doi.org/10.1101/gad.6.12b.2620\">10.1101/gad.6.12b.2620</a>","short":"Y. Sasai, R. Kageyama, Y. Tagawa, R. Shigemoto, S. Nakanishi, Genes and Development 6 (1992) 2620–2634."},"external_id":{"pmid":["1340473"]},"publication_identifier":{"issn":["0890-9369"]},"page":"2620 - 2634","doi":"10.1101/gad.6.12b.2620","publication":"Genes and Development","author":[{"first_name":"Yoshiki","last_name":"Sasai","full_name":"Sasai, Yoshiki"},{"full_name":"Kageyama, Ryoichiro","first_name":"Ryoichiro","last_name":"Kageyama"},{"last_name":"Tagawa","first_name":"Yoshiaki","full_name":"Tagawa, Yoshiaki"},{"orcid":"0000-0001-8761-9444","first_name":"Ryuichi","last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","full_name":"Shigemoto, Ryuichi"},{"full_name":"Nakanishi, Shigetada","last_name":"Nakanishi","first_name":"Shigetada"}],"acknowledgement":"We thank Professor Noboru Mizuno for his kind help with in situ hybridization experiments, Akira Uesugi and Dr. Chihiro\r\nAkazawa for photographic assistance, Drs. Elizabeth Knust and Jose A. Campos-Ortega for communicating their unpublished results, Dr. Shinji Fushiki for useful discussion, Dr. Mikio Nishizawa and Professor Shigekazu Nagata for pMNT, Dr. David Baltimore for the E47 expression vector, Drs. Yoichiro Nabeshima and Atsuko Fujisawa for the MyoD expression vector and the reporter plasmid with the MCK enhancer, and Dr. Makoto Ishibashi for his help in isolating the human E47 eDNA clone. This work was supported in part by research grants from the Ministry of Education, Science, and Culture of Japan. The publication costs of this article were defrayed in part by payment of page charges. This article must therefore be hereby marked \"advertisement\" in accordance with 18 USC section 1734 solely to indicate this fact. \r\n","year":"1992","intvolume":"         6","volume":6,"publist_id":"4364"}