{"title":"Tuning of the Zernike phase-plate for visualization of detailed ultrastructure in complex biological specimens","quality_controlled":0,"status":"public","publisher":"Academic Press","year":"2009","intvolume":"       168","volume":168,"date_created":"2018-12-11T11:58:03Z","publist_id":"4399","_id":"2502","abstract":[{"lang":"eng","text":"In order to acquire phase-contrast images with adequate contrast, conventional TEM requires large amount of defocus. Increasing the defocus improves the low-frequency components but attenuates the high-frequency ones. On the other hand, Zernike phase-contrast TEM (ZPC-TEM) can recover low-frequency components without losing the high-frequency ones under in-focus conditions. ZPC-TEM however, has another problem, especially in imaging of complex biological specimens such as cells and tissues; strong halos appear around specimen structures, and these halos hinder the interpretation of images. Due to this problem, the application of ZPC-TEM has been restricted to imaging of smaller particles. In order to improve the halo appearance, we fabricated a new quarter-wave thin film phase-plate with a smaller central hole and tested it on vitreous biological specimens. ZPC-TEM with the new plate could successfully visualize, in in-focus images, the intracellular fine features of cultured cells and brain tissues. This result indicates that reduction of the central hole diameter makes ZPC-TEM applicable on size scales ranging from protein particles to tissue sections. The application of ZPC-TEM to vitreous biological specimens will be a powerful method to advance the new field of imaging science for ultrastructures in close-to-physiological state."}],"extern":1,"date_published":"2009-12-01T00:00:00Z","citation":{"chicago":"Fukuda, Yoshiyuki, Yugo Fukazawa, Radostin Danev, Ryuichi Shigemoto, and Kuniaki Nagayama. “Tuning of the Zernike Phase-Plate for Visualization of Detailed Ultrastructure in Complex Biological Specimens.” <i>Journal of Structural Biology</i>. Academic Press, 2009. <a href=\"https://doi.org/10.1016/j.jsb.2009.08.011\">https://doi.org/10.1016/j.jsb.2009.08.011</a>.","apa":"Fukuda, Y., Fukazawa, Y., Danev, R., Shigemoto, R., &#38; Nagayama, K. (2009). Tuning of the Zernike phase-plate for visualization of detailed ultrastructure in complex biological specimens. <i>Journal of Structural Biology</i>. Academic Press. <a href=\"https://doi.org/10.1016/j.jsb.2009.08.011\">https://doi.org/10.1016/j.jsb.2009.08.011</a>","ista":"Fukuda Y, Fukazawa Y, Danev R, Shigemoto R, Nagayama K. 2009. Tuning of the Zernike phase-plate for visualization of detailed ultrastructure in complex biological specimens. Journal of Structural Biology. 168(3), 476–484.","ama":"Fukuda Y, Fukazawa Y, Danev R, Shigemoto R, Nagayama K. Tuning of the Zernike phase-plate for visualization of detailed ultrastructure in complex biological specimens. <i>Journal of Structural Biology</i>. 2009;168(3):476-484. doi:<a href=\"https://doi.org/10.1016/j.jsb.2009.08.011\">10.1016/j.jsb.2009.08.011</a>","short":"Y. Fukuda, Y. Fukazawa, R. Danev, R. Shigemoto, K. Nagayama, Journal of Structural Biology 168 (2009) 476–484.","mla":"Fukuda, Yoshiyuki, et al. “Tuning of the Zernike Phase-Plate for Visualization of Detailed Ultrastructure in Complex Biological Specimens.” <i>Journal of Structural Biology</i>, vol. 168, no. 3, Academic Press, 2009, pp. 476–84, doi:<a href=\"https://doi.org/10.1016/j.jsb.2009.08.011\">10.1016/j.jsb.2009.08.011</a>.","ieee":"Y. Fukuda, Y. Fukazawa, R. Danev, R. Shigemoto, and K. Nagayama, “Tuning of the Zernike phase-plate for visualization of detailed ultrastructure in complex biological specimens,” <i>Journal of Structural Biology</i>, vol. 168, no. 3. Academic Press, pp. 476–484, 2009."},"publication_status":"published","issue":"3","month":"12","date_updated":"2021-01-12T06:57:52Z","type":"journal_article","page":"476 - 484","doi":"10.1016/j.jsb.2009.08.011","author":[{"last_name":"Fukuda","first_name":"Yoshiyuki","full_name":"Fukuda, Yoshiyuki"},{"first_name":"Yugo","last_name":"Fukazawa","full_name":"Fukazawa, Yugo"},{"full_name":"Danev, Radostin S","first_name":"Radostin","last_name":"Danev"},{"last_name":"Shigemoto","first_name":"Ryuichi","full_name":"Ryuichi Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8761-9444"},{"full_name":"Nagayama, Kuniaki","first_name":"Kuniaki","last_name":"Nagayama"}],"publication":"Journal of Structural Biology","day":"01"}