{"month":"12","quality_controlled":"1","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","day":"09","_id":"2031","ec_funded":1,"language":[{"iso":"eng"}],"file_date_updated":"2020-07-14T12:45:26Z","file":[{"date_updated":"2020-07-14T12:45:26Z","date_created":"2018-12-12T10:14:41Z","checksum":"c240f915450d4ebe8f95043a2a8c7b1a","file_id":"5094","relation":"main_file","access_level":"open_access","file_size":2239563,"creator":"system","content_type":"application/pdf","file_name":"IST-2016-421-v1+1_e04057.full.pdf"}],"has_accepted_license":"1","publication_status":"published","date_updated":"2021-01-12T06:54:51Z","publist_id":"5041","intvolume":"         3","publisher":"eLife Sciences Publications","author":[{"last_name":"Arai","full_name":"Arai, Itaru","id":"32A73F6C-F248-11E8-B48F-1D18A9856A87","first_name":"Itaru"},{"orcid":"0000-0001-5001-4804","full_name":"Jonas, Peter M","last_name":"Jonas","first_name":"Peter M","id":"353C1B58-F248-11E8-B48F-1D18A9856A87"}],"abstract":[{"lang":"eng","text":"A puzzling property of synaptic transmission, originally established at the neuromuscular junction, is that the time course of transmitter release is independent of the extracellular Ca2+ concentration ([Ca2+]o), whereas the rate of release is highly [Ca2+]o-dependent. Here, we examine the time course of release at inhibitory basket cell-Purkinje cell synapses and show that it is independent of [Ca2+]o. Modeling of Ca2+-dependent transmitter release suggests that the invariant time course of release critically depends on tight coupling between Ca2+ channels and release sensors. Experiments with exogenous Ca2+ chelators reveal that channel-sensor coupling at basket cell-Purkinje cell synapses is very tight, with a mean distance of 10–20 nm. Thus, tight channel-sensor coupling provides a mechanistic explanation for the apparent [Ca2+]o independence of the time course of release."}],"oa":1,"date_created":"2018-12-11T11:55:19Z","title":"Nanodomain coupling explains Ca^2+ independence of transmitter release time course at a fast central synapse","doi":"10.7554/eLife.04057","department":[{"_id":"PeJo"}],"scopus_import":1,"status":"public","project":[{"grant_number":"P24909-B24","name":"Mechanisms of transmitter release at GABAergic synapses","call_identifier":"FWF","_id":"25C26B1E-B435-11E9-9278-68D0E5697425"},{"name":"Nanophysiology of fast-spiking, parvalbumin-expressing GABAergic interneurons","grant_number":"268548","call_identifier":"FP7","_id":"25C0F108-B435-11E9-9278-68D0E5697425"}],"oa_version":"Submitted Version","type":"journal_article","publication":"eLife","volume":3,"citation":{"ama":"Arai  itaru, Jonas PM. Nanodomain coupling explains Ca^2+ independence of transmitter release time course at a fast central synapse. <i>eLife</i>. 2014;3. doi:<a href=\"https://doi.org/10.7554/eLife.04057\">10.7554/eLife.04057</a>","ista":"Arai  itaru, Jonas PM. 2014. Nanodomain coupling explains Ca^2+ independence of transmitter release time course at a fast central synapse. eLife. 3.","chicago":"Arai, itaru, and Peter M Jonas. “Nanodomain Coupling Explains Ca^2+ Independence of Transmitter Release Time Course at a Fast Central Synapse.” <i>ELife</i>. eLife Sciences Publications, 2014. <a href=\"https://doi.org/10.7554/eLife.04057\">https://doi.org/10.7554/eLife.04057</a>.","ieee":"itaru Arai and P. M. Jonas, “Nanodomain coupling explains Ca^2+ independence of transmitter release time course at a fast central synapse,” <i>eLife</i>, vol. 3. eLife Sciences Publications, 2014.","mla":"Arai, itaru, and Peter M. Jonas. “Nanodomain Coupling Explains Ca^2+ Independence of Transmitter Release Time Course at a Fast Central Synapse.” <i>ELife</i>, vol. 3, eLife Sciences Publications, 2014, doi:<a href=\"https://doi.org/10.7554/eLife.04057\">10.7554/eLife.04057</a>.","short":"itaru Arai, P.M. Jonas, ELife 3 (2014).","apa":"Arai,  itaru, &#38; Jonas, P. M. (2014). Nanodomain coupling explains Ca^2+ independence of transmitter release time course at a fast central synapse. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.04057\">https://doi.org/10.7554/eLife.04057</a>"},"date_published":"2014-12-09T00:00:00Z","pubrep_id":"421","year":"2014","ddc":["570"]}