{"day":"01","status":"public","year":"2012","publisher":"Elsevier","page":"1785 - 1795","date_published":"2012-10-01T00:00:00Z","acknowledgement":"The work in authors' laboratory was funded by the Medical Research Council.","month":"10","date_created":"2018-12-11T11:55:00Z","citation":{"ista":"Efremov R, Sazanov LA. 2012. The coupling mechanism of respiratory complex i - A structural and evolutionary perspective. Biochimica et Biophysica Acta - Bioenergetics. 1817(10), 1785–1795.","chicago":"Efremov, Rouslan, and Leonid A Sazanov. “The Coupling Mechanism of Respiratory Complex i - A Structural and Evolutionary Perspective.” <i>Biochimica et Biophysica Acta - Bioenergetics</i>. Elsevier, 2012. <a href=\"https://doi.org/10.1016/j.bbabio.2012.02.015\">https://doi.org/10.1016/j.bbabio.2012.02.015</a>.","apa":"Efremov, R., &#38; Sazanov, L. A. (2012). The coupling mechanism of respiratory complex i - A structural and evolutionary perspective. <i>Biochimica et Biophysica Acta - Bioenergetics</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.bbabio.2012.02.015\">https://doi.org/10.1016/j.bbabio.2012.02.015</a>","short":"R. Efremov, L.A. Sazanov, Biochimica et Biophysica Acta - Bioenergetics 1817 (2012) 1785–1795.","mla":"Efremov, Rouslan, and Leonid A. Sazanov. “The Coupling Mechanism of Respiratory Complex i - A Structural and Evolutionary Perspective.” <i>Biochimica et Biophysica Acta - Bioenergetics</i>, vol. 1817, no. 10, Elsevier, 2012, pp. 1785–95, doi:<a href=\"https://doi.org/10.1016/j.bbabio.2012.02.015\">10.1016/j.bbabio.2012.02.015</a>.","ama":"Efremov R, Sazanov LA. The coupling mechanism of respiratory complex i - A structural and evolutionary perspective. <i>Biochimica et Biophysica Acta - Bioenergetics</i>. 2012;1817(10):1785-1795. doi:<a href=\"https://doi.org/10.1016/j.bbabio.2012.02.015\">10.1016/j.bbabio.2012.02.015</a>","ieee":"R. Efremov and L. A. Sazanov, “The coupling mechanism of respiratory complex i - A structural and evolutionary perspective,” <i>Biochimica et Biophysica Acta - Bioenergetics</i>, vol. 1817, no. 10. Elsevier, pp. 1785–1795, 2012."},"quality_controlled":0,"title":"The coupling mechanism of respiratory complex i - A structural and evolutionary perspective","_id":"1976","doi":"10.1016/j.bbabio.2012.02.015","author":[{"first_name":"Rouslan","full_name":"Efremov, Rouslan G","last_name":"Efremov"},{"id":"338D39FE-F248-11E8-B48F-1D18A9856A87","last_name":"Sazanov","orcid":"0000-0002-0977-7989","first_name":"Leonid A","full_name":"Leonid Sazanov"}],"abstract":[{"lang":"eng","text":"Complex I is a key enzyme of the respiratory chain in many organisms. This multi-protein complex with an intricate evolutionary history originated from the unification of prebuilt modules of hydrogenases and transporters. Using recently determined crystallographic structures of complex I we reanalyzed evolutionarily related complexes that couple oxidoreduction to trans-membrane ion translocation. Our analysis points to the previously unnoticed structural homology of the electron input module of formate dehydrogenlyases and subunit NuoG of complex I. We also show that all related to complex I hydrogenases likely operate via a conformation driven mechanism with structural changes generated in the conserved coupling site located at the interface of subunits NuoB/D/H. The coupling apparently originated once in evolutionary history, together with subunit NuoH joining hydrogenase and transport modules. Analysis of quinone oxidoreduction properties and the structure of complex I allows us to suggest a fully reversible coupling mechanism. Our model predicts that: 1) proton access to the ketone groups of the bound quinone is rigorously controlled by the protein, 2) the negative electric charge of the anionic ubiquinol head group is a major driving force for conformational changes."}],"issue":"10","publication":"Biochimica et Biophysica Acta - Bioenergetics","publication_status":"published","extern":1,"type":"review","date_updated":"2019-04-26T07:22:06Z","publist_id":"5108","volume":1817,"intvolume":"      1817"}