{"publisher":"Elsevier","year":"1996","article_type":"original","day":"11","date_published":"1996-01-11T00:00:00Z","pmid":1,"title":"Estimation of the H+/H- ratio of the reaction catalysed by the nicotinamide nucleotide transhydrogenase in chromatophores from over-expressing strains of Rhodospirillum rubrum and in liposomes inlaid with the purified bovine enzyme","quality_controlled":"1","issue":"1","oa_version":"None","publist_id":"5132","intvolume":"      1273","language":[{"iso":"eng"}],"page":"4 - 12","status":"public","external_id":{"pmid":["8573594 "]},"user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","article_processing_charge":"No","acknowledgement":"L.A.S. would like to thank the Wellcome Trust, and T.B., the Biotechnology and Biological Sciences Research Council, for financial support. We are very grateful to Nick Cotton for helpful advice.","citation":{"apa":"Bizouarn, T., Sazanov, L. A., Aubourg, S., &#38; Jackson, J. (1996). Estimation of the H+/H- ratio of the reaction catalysed by the nicotinamide nucleotide transhydrogenase in chromatophores from over-expressing strains of Rhodospirillum rubrum and in liposomes inlaid with the purified bovine enzyme. <i>Biochimica et Biophysica Acta - Bioenergetics</i>. Elsevier. <a href=\"https://doi.org/10.1016/0005-2728(95)00125-5\">https://doi.org/10.1016/0005-2728(95)00125-5</a>","chicago":"Bizouarn, Tania, Leonid A Sazanov, Sébastien Aubourg, and Julie Jackson. “Estimation of the H+/H- Ratio of the Reaction Catalysed by the Nicotinamide Nucleotide Transhydrogenase in Chromatophores from over-Expressing Strains of Rhodospirillum Rubrum and in Liposomes Inlaid with the Purified Bovine Enzyme.” <i>Biochimica et Biophysica Acta - Bioenergetics</i>. Elsevier, 1996. <a href=\"https://doi.org/10.1016/0005-2728(95)00125-5\">https://doi.org/10.1016/0005-2728(95)00125-5</a>.","ista":"Bizouarn T, Sazanov LA, Aubourg S, Jackson J. 1996. Estimation of the H+/H- ratio of the reaction catalysed by the nicotinamide nucleotide transhydrogenase in chromatophores from over-expressing strains of Rhodospirillum rubrum and in liposomes inlaid with the purified bovine enzyme. Biochimica et Biophysica Acta - Bioenergetics. 1273(1), 4–12.","ieee":"T. Bizouarn, L. A. Sazanov, S. Aubourg, and J. Jackson, “Estimation of the H+/H- ratio of the reaction catalysed by the nicotinamide nucleotide transhydrogenase in chromatophores from over-expressing strains of Rhodospirillum rubrum and in liposomes inlaid with the purified bovine enzyme,” <i>Biochimica et Biophysica Acta - Bioenergetics</i>, vol. 1273, no. 1. Elsevier, pp. 4–12, 1996.","short":"T. Bizouarn, L.A. Sazanov, S. Aubourg, J. Jackson, Biochimica et Biophysica Acta - Bioenergetics 1273 (1996) 4–12.","mla":"Bizouarn, Tania, et al. “Estimation of the H+/H- Ratio of the Reaction Catalysed by the Nicotinamide Nucleotide Transhydrogenase in Chromatophores from over-Expressing Strains of Rhodospirillum Rubrum and in Liposomes Inlaid with the Purified Bovine Enzyme.” <i>Biochimica et Biophysica Acta - Bioenergetics</i>, vol. 1273, no. 1, Elsevier, 1996, pp. 4–12, doi:<a href=\"https://doi.org/10.1016/0005-2728(95)00125-5\">10.1016/0005-2728(95)00125-5</a>.","ama":"Bizouarn T, Sazanov LA, Aubourg S, Jackson J. Estimation of the H+/H- ratio of the reaction catalysed by the nicotinamide nucleotide transhydrogenase in chromatophores from over-expressing strains of Rhodospirillum rubrum and in liposomes inlaid with the purified bovine enzyme. <i>Biochimica et Biophysica Acta - Bioenergetics</i>. 1996;1273(1):4-12. doi:<a href=\"https://doi.org/10.1016/0005-2728(95)00125-5\">10.1016/0005-2728(95)00125-5</a>"},"date_created":"2018-12-11T11:54:53Z","month":"01","author":[{"first_name":"Tania","full_name":"Bizouarn, Tania","last_name":"Bizouarn"},{"last_name":"Sazanov","id":"338D39FE-F248-11E8-B48F-1D18A9856A87","first_name":"Leonid A","full_name":"Sazanov, Leonid A","orcid":"0000-0002-0977-7989"},{"last_name":"Aubourg","full_name":"Aubourg, Sébastien","first_name":"Sébastien"},{"last_name":"Jackson","first_name":"Julie","full_name":"Jackson, Julie"}],"doi":"10.1016/0005-2728(95)00125-5","_id":"1952","publication_status":"published","publication":"Biochimica et Biophysica Acta - Bioenergetics","abstract":[{"text":"Two strains of Rhodospirillum rubrum were constructed in which, by a gene dosage effect, the transhydrogenase activity of isolated chromatophores was increased 7-10-fold and 15-20-fold, respectively. The H+/H- ratio (the ratio of protons translocated per hydride ion equivalent transferred from NADPH to an NAD+ analogue, acetyl pyridine adenine dinucleotide), determined by a spectroscopic technique, was approximately 1.0 for chromatophores from the over-expressing strains, but was only approximately 0.6 for wild-type chromatophores. Highly-coupled proteoliposomes were prepared containing purified transhydrogenase from beef-heart mitochondria. Using the same technique, the H+/H- ratio was close to 1.0 for these proteoliposomes. It is suggested that the mechanistic H+/H- ratio is indeed unity, but that a low ratio is obtained in wild-type chromatophores because of inhomogeneity in the vesicle population.","lang":"eng"}],"volume":1273,"date_updated":"2022-08-16T12:35:22Z","type":"journal_article","extern":"1","publication_identifier":{"issn":["0005-2728"]}}