{"date_updated":"2021-01-12T06:53:43Z","month":"02","day":"01","type":"journal_article","abstract":[{"lang":"eng","text":"Cultured mammalian cells essential are model systems in basic biology research, production platforms of proteins for medical use, and testbeds in synthetic biology. Flavin cofactors, in particular flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), are critical for cellular redox reactions and sense light in naturally occurring photoreceptors and optogenetic tools. Here, we quantified flavin contents of commonly used mammalian cell lines. We first compared three procedures for extraction of free and noncovalently protein-bound flavins and verified extraction using fluorescence spectroscopy. For separation, two CE methods with different BGEs were established, and detection was performed by LED-induced fluorescence with limit of detections (LODs 0.5-3.8 nM). We found that riboflavin (RF), FMN, and FAD contents varied significantly between cell lines. RF (3.1-14 amol/cell) and FAD (2.2-17.0 amol/cell) were the predominant flavins, while FMN (0.46-3.4 amol/cell) was found at markedly lower levels. Observed flavin contents agree with those previously extracted from mammalian tissues, yet reduced forms of RF were detected that were not described previously. Quantification of flavins in mammalian cell lines will allow a better understanding of cellular redox reactions and optogenetic tools."}],"department":[{"_id":"HaJa"}],"issue":"4","date_published":"2015-02-01T00:00:00Z","publication_status":"published","scopus_import":1,"publisher":"Wiley","date_created":"2018-12-11T11:54:26Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa_version":"None","status":"public","quality_controlled":"1","title":"Quantification of riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in mammalian model cells by CE with LED-induced fluorescence detection","page":"518 - 525","doi":"10.1002/elps.201400451","author":[{"full_name":"Hühner, Jens","last_name":"Hühner","first_name":"Jens"},{"orcid":"0000-0002-5409-8571","first_name":"Álvaro","last_name":"Inglés Prieto","full_name":"Inglés Prieto, Álvaro","id":"2A9DB292-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Neusüß, Christian","last_name":"Neusüß","first_name":"Christian"},{"first_name":"Michael","last_name":"Lämmerhofer","full_name":"Lämmerhofer, Michael"},{"full_name":"Janovjak, Harald L","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","first_name":"Harald L","last_name":"Janovjak","orcid":"0000-0002-8023-9315"}],"publication":"Electrophoresis","pubrep_id":"836","language":[{"iso":"eng"}],"_id":"1867","ec_funded":1,"citation":{"short":"J. Hühner, Á. Inglés Prieto, C. Neusüß, M. Lämmerhofer, H.L. Janovjak, Electrophoresis 36 (2015) 518–525.","ama":"Hühner J, Inglés Prieto Á, Neusüß C, Lämmerhofer M, Janovjak HL. Quantification of riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in mammalian model cells by CE with LED-induced fluorescence detection. <i>Electrophoresis</i>. 2015;36(4):518-525. doi:<a href=\"https://doi.org/10.1002/elps.201400451\">10.1002/elps.201400451</a>","apa":"Hühner, J., Inglés Prieto, Á., Neusüß, C., Lämmerhofer, M., &#38; Janovjak, H. L. (2015). Quantification of riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in mammalian model cells by CE with LED-induced fluorescence detection. <i>Electrophoresis</i>. Wiley. <a href=\"https://doi.org/10.1002/elps.201400451\">https://doi.org/10.1002/elps.201400451</a>","chicago":"Hühner, Jens, Álvaro Inglés Prieto, Christian Neusüß, Michael Lämmerhofer, and Harald L Janovjak. “Quantification of Riboflavin, Flavin Mononucleotide, and Flavin Adenine Dinucleotide in Mammalian Model Cells by CE with LED-Induced Fluorescence Detection.” <i>Electrophoresis</i>. Wiley, 2015. <a href=\"https://doi.org/10.1002/elps.201400451\">https://doi.org/10.1002/elps.201400451</a>.","ista":"Hühner J, Inglés Prieto Á, Neusüß C, Lämmerhofer M, Janovjak HL. 2015. Quantification of riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in mammalian model cells by CE with LED-induced fluorescence detection. Electrophoresis. 36(4), 518–525.","ieee":"J. Hühner, Á. Inglés Prieto, C. Neusüß, M. Lämmerhofer, and H. L. Janovjak, “Quantification of riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in mammalian model cells by CE with LED-induced fluorescence detection,” <i>Electrophoresis</i>, vol. 36, no. 4. Wiley, pp. 518–525, 2015.","mla":"Hühner, Jens, et al. “Quantification of Riboflavin, Flavin Mononucleotide, and Flavin Adenine Dinucleotide in Mammalian Model Cells by CE with LED-Induced Fluorescence Detection.” <i>Electrophoresis</i>, vol. 36, no. 4, Wiley, 2015, pp. 518–25, doi:<a href=\"https://doi.org/10.1002/elps.201400451\">10.1002/elps.201400451</a>."},"year":"2015","publist_id":"5230","volume":36,"intvolume":"        36","project":[{"call_identifier":"FP7","name":"Microbial Ion Channels for Synthetic Neurobiology","_id":"25548C20-B435-11E9-9278-68D0E5697425","grant_number":"303564"},{"name":"In situ real-time imaging of neurotransmitter signaling using designer optical sensors (HFSP Young Investigator)","_id":"255BFFFA-B435-11E9-9278-68D0E5697425","grant_number":"RGY0084/2012"}]}