{"publisher":"Nature Publishing Group","scopus_import":1,"oa":1,"oa_version":"Submitted Version","date_created":"2018-12-11T11:53:25Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Light-assisted small-molecule screening against protein kinases","quality_controlled":"1","related_material":{"record":[{"id":"418","relation":"dissertation_contains","status":"public"}]},"status":"public","month":"10","date_updated":"2023-09-07T12:49:09Z","file":[{"content_type":"application/pdf","access_level":"open_access","creator":"system","file_name":"IST-2017-837-v1+1_ingles-prieto.pdf","checksum":"e9fb251dfcb7cd209b83f17867e61321","relation":"main_file","file_id":"4842","date_created":"2018-12-12T10:10:51Z","file_size":1308364,"date_updated":"2020-07-14T12:45:12Z"}],"type":"journal_article","day":"12","department":[{"_id":"HaJa"},{"_id":"LifeSc"}],"abstract":[{"text":"High-throughput live-cell screens are intricate elements of systems biology studies and drug discovery pipelines. Here, we demonstrate an optogenetics-assisted method that avoids the need for chemical activators and reporters, reduces the number of operational steps and increases information content in a cell-based small-molecule screen against human protein kinases, including an orphan receptor tyrosine kinase. This blueprint for all-optical screening can be adapted to many drug targets and cellular processes.","lang":"eng"}],"publication_status":"published","date_published":"2015-10-12T00:00:00Z","issue":"12","year":"2015","intvolume":"        11","volume":11,"publist_id":"5471","ddc":["571"],"project":[{"call_identifier":"FP7","grant_number":"303564","_id":"25548C20-B435-11E9-9278-68D0E5697425","name":"Microbial Ion Channels for Synthetic Neurobiology"},{"_id":"255BFFFA-B435-11E9-9278-68D0E5697425","name":"In situ real-time imaging of neurotransmitter signaling using designer optical sensors (HFSP Young Investigator)","grant_number":"RGY0084/2012"},{"_id":"255A6082-B435-11E9-9278-68D0E5697425","name":"Molecular Drug Targets","grant_number":"W1232-B24","call_identifier":"FWF"}],"acknowledgement":"This work was supported by grants from the European Union Seventh Framework Programme (CIG-303564 to H.J. and ERC-StG-311166 to S.M.B.N.), the Human Frontier Science Program (RGY0084_2012 to H.J.) and the Herzfelder Foundation (to M.G.). A.I.-P. was supported by a Ramon Areces fellowship, and E.R. by the graduate program MolecularDrugTargets (Austrian Science Fund (FWF): W 1232) and a FemTech fellowship (3580812 Austrian Research Promotion Agency).","file_date_updated":"2020-07-14T12:45:12Z","page":"952 - 954","doi":"10.1038/nchembio.1933","author":[{"orcid":"0000-0002-5409-8571","id":"2A9DB292-F248-11E8-B48F-1D18A9856A87","full_name":"Inglés Prieto, Álvaro","last_name":"Inglés Prieto","first_name":"Álvaro"},{"last_name":"Gschaider-Reichhart","first_name":"Eva","full_name":"Gschaider-Reichhart, Eva","id":"3FEE232A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7218-7738"},{"last_name":"Muellner","first_name":"Markus","full_name":"Muellner, Markus"},{"full_name":"Nowak, Matthias","id":"30845DAA-F248-11E8-B48F-1D18A9856A87","first_name":"Matthias","last_name":"Nowak"},{"full_name":"Nijman, Sebastian","last_name":"Nijman","first_name":"Sebastian"},{"last_name":"Grusch","first_name":"Michael","full_name":"Grusch, Michael"},{"orcid":"0000-0002-8023-9315","full_name":"Janovjak, Harald L","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","last_name":"Janovjak","first_name":"Harald L"}],"has_accepted_license":"1","publication":"Nature Chemical Biology","pubrep_id":"837","language":[{"iso":"eng"}],"_id":"1678","ec_funded":1,"citation":{"ama":"Inglés Prieto Á, Gschaider-Reichhart E, Muellner M, et al. Light-assisted small-molecule screening against protein kinases. <i>Nature Chemical Biology</i>. 2015;11(12):952-954. doi:<a href=\"https://doi.org/10.1038/nchembio.1933\">10.1038/nchembio.1933</a>","ista":"Inglés Prieto Á, Gschaider-Reichhart E, Muellner M, Nowak M, Nijman S, Grusch M, Janovjak HL. 2015. Light-assisted small-molecule screening against protein kinases. Nature Chemical Biology. 11(12), 952–954.","apa":"Inglés Prieto, Á., Gschaider-Reichhart, E., Muellner, M., Nowak, M., Nijman, S., Grusch, M., &#38; Janovjak, H. L. (2015). Light-assisted small-molecule screening against protein kinases. <i>Nature Chemical Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nchembio.1933\">https://doi.org/10.1038/nchembio.1933</a>","chicago":"Inglés Prieto, Álvaro, Eva Gschaider-Reichhart, Markus Muellner, Matthias Nowak, Sebastian Nijman, Michael Grusch, and Harald L Janovjak. “Light-Assisted Small-Molecule Screening against Protein Kinases.” <i>Nature Chemical Biology</i>. Nature Publishing Group, 2015. <a href=\"https://doi.org/10.1038/nchembio.1933\">https://doi.org/10.1038/nchembio.1933</a>.","short":"Á. Inglés Prieto, E. Gschaider-Reichhart, M. Muellner, M. Nowak, S. Nijman, M. Grusch, H.L. Janovjak, Nature Chemical Biology 11 (2015) 952–954.","mla":"Inglés Prieto, Álvaro, et al. “Light-Assisted Small-Molecule Screening against Protein Kinases.” <i>Nature Chemical Biology</i>, vol. 11, no. 12, Nature Publishing Group, 2015, pp. 952–54, doi:<a href=\"https://doi.org/10.1038/nchembio.1933\">10.1038/nchembio.1933</a>.","ieee":"Á. Inglés Prieto <i>et al.</i>, “Light-assisted small-molecule screening against protein kinases,” <i>Nature Chemical Biology</i>, vol. 11, no. 12. Nature Publishing Group, pp. 952–954, 2015."}}