Solution structure of CCL19 and identification of overlapping CCR7 and PSGL-1 binding sites - Veldkamp, Christopher; Kiermaier, Eva; Gabel Eissens, Skylar; Gillitzer, Miranda; Lippner, David; Disilvio, Frank; Mueller, Casey; Wantuch, Paeton; Chaffee, Gary; Famiglietti, Michael; Zgoba, Danielle; Bailey, Asha; et al. (Biochemistry, 2015)
CCL19 and CCL21 are chemokines involved in the trafficking of immune cells, particularly within the lymphatic system, through activation of CCR7. Concurrent expression of PSGL-1 and CCR7 in naive T-cells enhances recruitment of these cells to secondary lymphoid organs by CCL19 and CCL21. Here the solution structure of CCL19 is reported. It contains a canonical chemokine domain. Chemical shift mapping shows the N-termini of PSGL-1 and CCR7 have overlapping binding sites for CCL19 and binding is competitive. Implications for the mechanism of PSGL-1's enhancement of resting T-cell recruitment are discussed.
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Keywords: article