{"publisher":"Rockefeller University Press","oa":1,"oa_version":"Published Version","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2024-01-10T10:45:55Z","quality_controlled":"1","title":"Clathrin coats partially preassemble and subsequently bend during endocytosis","status":"public","article_processing_charge":"No","month":"02","tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"date_updated":"2024-01-16T10:17:05Z","pmid":1,"file":[{"date_updated":"2024-01-16T10:15:09Z","date_created":"2024-01-16T10:15:09Z","file_size":5678069,"file_id":"14811","success":1,"relation":"main_file","checksum":"505d5cac36c14b073b68c7fed1a92bd3","file_name":"2023_JCB_Mund.pdf","creator":"dernst","access_level":"open_access","content_type":"application/pdf"}],"type":"journal_article","day":"03","department":[{"_id":"AnSa"}],"abstract":[{"text":"Eukaryotic cells use clathrin-mediated endocytosis to take up a large range of extracellular cargo. During endocytosis, a clathrin coat forms on the plasma membrane, but it remains controversial when and how it is remodeled into a spherical vesicle.\r\nHere, we use 3D superresolution microscopy to determine the precise geometry of the clathrin coat at large numbers of endocytic sites. Through pseudo-temporal sorting, we determine the average trajectory of clathrin remodeling during endocytosis. We find that clathrin coats assemble first on flat membranes to 50% of the coat area before they become rapidly and continuously bent, and this mechanism is confirmed in three cell lines. We introduce the cooperative curvature model, which is based on positive feedback for curvature generation. It accurately describes the measured shapes and dynamics of the clathrin coat and could represent a general mechanism for clathrin coat remodeling on the plasma membrane.","lang":"eng"}],"date_published":"2023-02-03T00:00:00Z","publication_status":"published","issue":"3","year":"2023","intvolume":"       222","volume":222,"ddc":["570"],"file_date_updated":"2024-01-16T10:15:09Z","acknowledgement":"We thank the entire Ries and Kaksonen labs for fruitful discussions and support. This work was supported by the European Research Council (ERC CoG-724489 to J. Ries), the National Institutes of Health Common Fund 4D Nucleome Program (Grant U01 to J. Ries), the Human Frontier Science Program (RGY0065/2017 to J. Ries), the EMBL Interdisciplinary Postdoc Programme (EIPOD) under Marie Curie Actions COFUND (Grant 229597 to O. Avinoam), the European Molecular Biology Laboratory (M. Mund, A. Tschanz, Y.-L. Wu and J. Ries), and the Swiss National Science Foundation (grant 310030B_182825 and NCCR Chemical Biology to M. Kaksonen). O. Avinoam is an incumbent of the Miriam Berman Presidential Development Chair.","keyword":["Cell Biology"],"isi":1,"external_id":{"pmid":["36734980"],"isi":["000978065000001"]},"publication_identifier":{"issn":["0021-9525"],"eissn":["1540-8140"]},"doi":"10.1083/jcb.202206038","has_accepted_license":"1","author":[{"first_name":"Markus","last_name":"Mund","full_name":"Mund, Markus"},{"first_name":"Aline","last_name":"Tschanz","full_name":"Tschanz, Aline"},{"full_name":"Wu, Yu-Le","first_name":"Yu-Le","last_name":"Wu"},{"orcid":"0000-0001-8501-6017","id":"a0270b37-8f1a-11ec-95c7-8e710c59a4f3","full_name":"Frey, Felix F","last_name":"Frey","first_name":"Felix F"},{"full_name":"Mehl, Johanna L.","last_name":"Mehl","first_name":"Johanna L."},{"full_name":"Kaksonen, Marko","last_name":"Kaksonen","first_name":"Marko"},{"full_name":"Avinoam, Ori","first_name":"Ori","last_name":"Avinoam"},{"full_name":"Schwarz, Ulrich S.","first_name":"Ulrich S.","last_name":"Schwarz"},{"full_name":"Ries, Jonas","last_name":"Ries","first_name":"Jonas"}],"publication":"Journal of Cell Biology","language":[{"iso":"eng"}],"_id":"14788","article_number":"e202206038","citation":{"ieee":"M. Mund <i>et al.</i>, “Clathrin coats partially preassemble and subsequently bend during endocytosis,” <i>Journal of Cell Biology</i>, vol. 222, no. 3. Rockefeller University Press, 2023.","mla":"Mund, Markus, et al. “Clathrin Coats Partially Preassemble and Subsequently Bend during Endocytosis.” <i>Journal of Cell Biology</i>, vol. 222, no. 3, e202206038, Rockefeller University Press, 2023, doi:<a href=\"https://doi.org/10.1083/jcb.202206038\">10.1083/jcb.202206038</a>.","short":"M. Mund, A. Tschanz, Y.-L. Wu, F.F. Frey, J.L. Mehl, M. Kaksonen, O. Avinoam, U.S. Schwarz, J. Ries, Journal of Cell Biology 222 (2023).","chicago":"Mund, Markus, Aline Tschanz, Yu-Le Wu, Felix F Frey, Johanna L. Mehl, Marko Kaksonen, Ori Avinoam, Ulrich S. Schwarz, and Jonas Ries. “Clathrin Coats Partially Preassemble and Subsequently Bend during Endocytosis.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2023. <a href=\"https://doi.org/10.1083/jcb.202206038\">https://doi.org/10.1083/jcb.202206038</a>.","ista":"Mund M, Tschanz A, Wu Y-L, Frey FF, Mehl JL, Kaksonen M, Avinoam O, Schwarz US, Ries J. 2023. Clathrin coats partially preassemble and subsequently bend during endocytosis. Journal of Cell Biology. 222(3), e202206038.","apa":"Mund, M., Tschanz, A., Wu, Y.-L., Frey, F. F., Mehl, J. L., Kaksonen, M., … Ries, J. (2023). Clathrin coats partially preassemble and subsequently bend during endocytosis. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.202206038\">https://doi.org/10.1083/jcb.202206038</a>","ama":"Mund M, Tschanz A, Wu Y-L, et al. Clathrin coats partially preassemble and subsequently bend during endocytosis. <i>Journal of Cell Biology</i>. 2023;222(3). doi:<a href=\"https://doi.org/10.1083/jcb.202206038\">10.1083/jcb.202206038</a>"},"article_type":"original"}