{"date_published":"2023-11-21T00:00:00Z","department":[{"_id":"FlSc"}],"abstract":[{"lang":"eng","text":"Regulation of the Arp2/3 complex is required for productive nucleation of branched actin networks. An emerging aspect of regulation is the incorporation of subunit isoforms into the Arp2/3 complex. Specifically, both ArpC5 subunit isoforms, ArpC5 and ArpC5L, have been reported to fine-tune nucleation activity and branch junction stability. We have combined reverse genetics and cellular structural biology to describe how ArpC5 and ArpC5L differentially affect cell migration. Both define the structural stability of ArpC1 in branch junctions and, in turn, by determining protrusion characteristics, affect protein dynamics and actin network ultrastructure. ArpC5 isoforms also affect the positioning of members of the Ena/Vasodilator-stimulated phosphoprotein (VASP) family of actin filament elongators, which mediate ArpC5 isoform–specific effects on the actin assembly level. Our results suggest that ArpC5 and Ena/VASP proteins are part of a signaling pathway enhancing cell migration.\r\n"}],"file":[{"access_level":"open_access","creator":"fschur","file_name":"Figure2.zip","content_type":"application/zip","checksum":"e9bab797b44614f144a5b02d9636f8c3","relation":"main_file","file_size":1581687449,"date_created":"2023-11-20T10:27:17Z","success":1,"file_id":"14570","date_updated":"2023-11-20T10:27:17Z"},{"date_updated":"2023-11-20T10:29:18Z","file_id":"14571","success":1,"date_created":"2023-11-20T10:29:18Z","file_size":116088565,"relation":"main_file","checksum":"4efd388cccd03c549fc90f6e46d37006","content_type":"application/zip","file_name":"SupplementaryFigure3.zip","creator":"fschur","access_level":"open_access"},{"checksum":"bdeb232dc94d0c22a3f7e0d18189ce89","relation":"main_file","content_type":"application/zip","creator":"fschur","access_level":"open_access","file_name":"Figure5.zip","date_updated":"2023-11-20T10:44:39Z","success":1,"file_id":"14572","date_created":"2023-11-20T10:44:39Z","file_size":5154614201},{"date_updated":"2023-11-20T10:46:00Z","date_created":"2023-11-20T10:46:00Z","file_size":1277893286,"success":1,"file_id":"14573","checksum":"83aee17d621a05d865f68f39c8892d27","relation":"main_file","access_level":"open_access","creator":"fschur","file_name":"SupplementaryFigure7.zip","content_type":"application/zip"},{"date_updated":"2023-11-20T10:46:08Z","date_created":"2023-11-20T10:46:08Z","file_size":228485124,"success":1,"file_id":"14574","checksum":"fb9beb6fe15c8dac6679dd02044d2ea6","relation":"main_file","creator":"fschur","access_level":"open_access","file_name":"SupplementaryFigure9.zip","content_type":"application/zip"},{"relation":"main_file","checksum":"4f3644e5feabe4824486d56885bb79fe","file_name":"SupplementaryFigure10.zip","creator":"fschur","access_level":"open_access","content_type":"application/zip","date_updated":"2023-11-20T10:46:32Z","file_size":1226788198,"date_created":"2023-11-20T10:46:32Z","file_id":"14575","success":1},{"date_updated":"2023-11-20T10:46:17Z","date_created":"2023-11-20T10:46:17Z","file_size":277577131,"file_id":"14576","success":1,"relation":"main_file","checksum":"96167f722ed0ca78e30681cd1573b9d7","file_name":"SupplementaryFigure11.zip","creator":"fschur","access_level":"open_access","content_type":"application/zip"},{"date_updated":"2023-11-20T10:46:29Z","file_id":"14577","success":1,"date_created":"2023-11-20T10:46:29Z","file_size":591483468,"relation":"main_file","checksum":"d1e03c9805c18cfbc2e9fdf38a9f556f","content_type":"application/zip","file_name":"SupplementaryFigure15.zip","creator":"fschur","access_level":"open_access"},{"relation":"main_file","checksum":"4d437c04fdb3c1e699618063c4bd21c3","content_type":"application/zip","file_name":"SupplementaryFigure17.zip","creator":"fschur","access_level":"open_access","date_updated":"2023-11-20T10:47:00Z","file_id":"14578","success":1,"file_size":1709528579,"date_created":"2023-11-20T10:47:00Z"},{"file_size":1920765280,"date_created":"2023-11-20T11:26:36Z","success":1,"file_id":"14581","date_updated":"2023-11-20T11:26:36Z","creator":"fschur","access_level":"open_access","file_name":"SupplementaryFigure4.zip","content_type":"application/zip","checksum":"967b5378a4f16c43f490eae328afe50e","relation":"main_file"},{"checksum":"11899986cf0b471d258fe168ee33a3ea","relation":"main_file","creator":"fschur","access_level":"open_access","file_name":"Figure1_partA.zip","content_type":"application/zip","date_updated":"2023-11-20T11:38:12Z","date_created":"2023-11-20T11:38:12Z","file_size":3013566196,"success":1,"file_id":"14583"},{"file_size":3250260203,"date_created":"2023-11-20T11:43:23Z","file_id":"14584","success":1,"date_updated":"2023-11-20T11:43:23Z","file_name":"Figure1_partB.zip","creator":"fschur","access_level":"open_access","content_type":"application/zip","relation":"main_file","checksum":"c452afe1ab506d58d32e601d5b3878bb"},{"success":1,"file_id":"14585","file_size":1460,"date_created":"2023-11-20T11:49:58Z","date_updated":"2023-11-20T11:49:58Z","content_type":"text/rtf","access_level":"open_access","creator":"fschur","file_name":"ReadMe.rtf","checksum":"223c98eceecbe65dd268f4f363a620d8","relation":"main_file"}],"license":"https://creativecommons.org/licenses/by-sa/4.0/","type":"research_data","day":"21","month":"11","article_processing_charge":"No","tmp":{"short":"CC BY-SA (4.0)","image":"/images/cc_by_sa.png","name":"Creative Commons Attribution-ShareAlike 4.0 International Public License (CC BY-SA 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-sa/4.0/legalcode"},"date_updated":"2023-11-21T08:05:34Z","title":"Research data of the publication \"ArpC5 isoforms regulate Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning\"","related_material":{"record":[{"relation":"used_in_publication","id":"12334","status":"public"}]},"status":"public","contributor":[{"contributor_type":"researcher","orcid":"0000-0001-7149-769X","id":"404F5528-F248-11E8-B48F-1D18A9856A87","last_name":"Fäßler","first_name":"Florian"},{"contributor_type":"researcher","id":"305ab18b-dc7d-11ea-9b2f-b58195228ea2","first_name":"Manjunath","last_name":"Javoor"},{"id":"3B12E2E6-F248-11E8-B48F-1D18A9856A87","first_name":"Julia","last_name":"Datler","contributor_type":"researcher","orcid":"0000-0002-3616-8580"},{"last_name":"Döring","first_name":"Hermann","contributor_type":"researcher"},{"contributor_type":"researcher","id":"b9d234ba-9e33-11ed-95b6-cd561df280e6","last_name":"Hofer","first_name":"Florian"},{"last_name":"Dimchev","first_name":"Georgi A","id":"38C393BE-F248-11E8-B48F-1D18A9856A87","contributor_type":"researcher","orcid":"0000-0001-8370-6161"},{"first_name":"Victor-Valentin","last_name":"Hodirnau","id":"3661B498-F248-11E8-B48F-1D18A9856A87","contributor_type":"researcher"},{"contributor_type":"researcher","first_name":"Jan","last_name":"Faix"},{"first_name":"Klemens","last_name":"Rottner","contributor_type":"researcher"},{"id":"48AD8942-F248-11E8-B48F-1D18A9856A87","first_name":"Florian KM","last_name":"Schur","orcid":"0000-0003-4790-8078","contributor_type":"researcher"}],"oa":1,"oa_version":"Published Version","date_created":"2023-11-20T09:22:33Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Institute of Science and Technology Austria","citation":{"short":"F.K. Schur, (2023).","apa":"Schur, F. K. (2023). Research data of the publication “ArpC5 isoforms regulate Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning.” Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:14562\">https://doi.org/10.15479/AT:ISTA:14562</a>","ista":"Schur FK. 2023. Research data of the publication ‘ArpC5 isoforms regulate Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning’, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:14562\">10.15479/AT:ISTA:14562</a>.","chicago":"Schur, Florian KM. “Research Data of the Publication ‘ArpC5 Isoforms Regulate Arp2/3 Complex-Dependent Protrusion through Differential Ena/VASP Positioning.’” Institute of Science and Technology Austria, 2023. <a href=\"https://doi.org/10.15479/AT:ISTA:14562\">https://doi.org/10.15479/AT:ISTA:14562</a>.","ama":"Schur FK. Research data of the publication “ArpC5 isoforms regulate Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning.” 2023. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:14562\">10.15479/AT:ISTA:14562</a>","ieee":"F. K. Schur, “Research data of the publication ‘ArpC5 isoforms regulate Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning.’” Institute of Science and Technology Austria, 2023.","mla":"Schur, Florian KM. <i>Research Data of the Publication “ArpC5 Isoforms Regulate Arp2/3 Complex-Dependent Protrusion through Differential Ena/VASP Positioning.”</i> Institute of Science and Technology Austria, 2023, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:14562\">10.15479/AT:ISTA:14562</a>."},"_id":"14562","doi":"10.15479/AT:ISTA:14562","has_accepted_license":"1","author":[{"id":"48AD8942-F248-11E8-B48F-1D18A9856A87","full_name":"Schur, Florian KM","last_name":"Schur","first_name":"Florian KM","orcid":"0000-0003-4790-8078"}],"acknowledgement":"We would like to thank K. von Peinen and B. Denker (Helmholtz Centre for Infection Research, Braunschweig, Germany) for experimental and technical assistance, respectively.\r\nFunding: This research was supported by the Scientific Service Units (SSUs) of ISTA through resources provided by Scientific Computing (SciComp), the Life Science Facility (LSF), the Imaging and Optics facility (IOF), and the Electron Microscopy Facility (EMF). We acknowledge support from ISTA and from the Austrian Science Fund (FWF) (P33367) to F.K.M.S., from the Research Training Group GRK2223 and the Helmholtz Society to K.R,. and from the Deutsche Forschungsgemeinschaft (DFG) to J.F. and K.R.","file_date_updated":"2023-11-20T11:49:58Z","ddc":["570"],"project":[{"grant_number":"P33367","name":"Structure and isoform diversity of the Arp2/3 complex","_id":"9B954C5C-BA93-11EA-9121-9846C619BF3A"}],"year":"2023","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"ScienComp"},{"_id":"EM-Fac"}]}