{"_id":"14287","month":"03","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","quality_controlled":"1","day":"24","article_number":"eaam5488","language":[{"iso":"eng"}],"publication_status":"published","date_updated":"2023-11-07T12:33:05Z","extern":"1","abstract":[{"text":"We describe an approach to bottom-up fabrication that allows integration of the functional diversity of proteins into designed three-dimensional structural frameworks. A set of custom staple proteins based on transcription activator–like effector proteins folds a double-stranded DNA template into a user-defined shape. Each staple protein is designed to recognize and closely link two distinct double-helical DNA sequences at separate positions on the template. We present design rules for constructing megadalton-scale DNA-protein hybrid shapes; introduce various structural motifs, such as custom curvature, corners, and vertices; and describe principles for creating multilayer DNA-protein objects with enhanced rigidity. We demonstrate self-assembly of our hybrid nanostructures in one-pot mixtures that include the genetic information for the designed proteins, the template DNA, RNA polymerase, ribosomes, and cofactors for transcription and translation.","lang":"eng"}],"date_created":"2023-09-06T12:08:55Z","publication_identifier":{"issn":["0036-8075"],"eissn":["1095-9203"]},"intvolume":"       355","publisher":"American Association for the Advancement of Science","author":[{"last_name":"Praetorius","full_name":"Praetorius, Florian M","id":"dfec9381-4341-11ee-8fd8-faa02bba7d62","first_name":"Florian M"},{"full_name":"Dietz, Hendrik","last_name":"Dietz","first_name":"Hendrik"}],"pmid":1,"article_processing_charge":"No","external_id":{"pmid":["28336611"]},"title":"Self-assembly of genetically encoded DNA-protein hybrid nanoscale shapes","article_type":"original","doi":"10.1126/science.aam5488","oa_version":"None","issue":"6331","scopus_import":"1","status":"public","year":"2017","publication":"Science","type":"journal_article","volume":355,"date_published":"2017-03-24T00:00:00Z","citation":{"ama":"Praetorius FM, Dietz H. Self-assembly of genetically encoded DNA-protein hybrid nanoscale shapes. <i>Science</i>. 2017;355(6331). doi:<a href=\"https://doi.org/10.1126/science.aam5488\">10.1126/science.aam5488</a>","ista":"Praetorius FM, Dietz H. 2017. Self-assembly of genetically encoded DNA-protein hybrid nanoscale shapes. Science. 355(6331), eaam5488.","ieee":"F. M. Praetorius and H. Dietz, “Self-assembly of genetically encoded DNA-protein hybrid nanoscale shapes,” <i>Science</i>, vol. 355, no. 6331. American Association for the Advancement of Science, 2017.","mla":"Praetorius, Florian M., and Hendrik Dietz. “Self-Assembly of Genetically Encoded DNA-Protein Hybrid Nanoscale Shapes.” <i>Science</i>, vol. 355, no. 6331, eaam5488, American Association for the Advancement of Science, 2017, doi:<a href=\"https://doi.org/10.1126/science.aam5488\">10.1126/science.aam5488</a>.","short":"F.M. Praetorius, H. Dietz, Science 355 (2017).","chicago":"Praetorius, Florian M, and Hendrik Dietz. “Self-Assembly of Genetically Encoded DNA-Protein Hybrid Nanoscale Shapes.” <i>Science</i>. American Association for the Advancement of Science, 2017. <a href=\"https://doi.org/10.1126/science.aam5488\">https://doi.org/10.1126/science.aam5488</a>.","apa":"Praetorius, F. M., &#38; Dietz, H. (2017). Self-assembly of genetically encoded DNA-protein hybrid nanoscale shapes. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.aam5488\">https://doi.org/10.1126/science.aam5488</a>"}}