{"language":[{"iso":"eng"}],"intvolume":"       353","type":"journal_article","volume":353,"publist_id":"5911","date_updated":"2021-01-12T06:50:01Z","abstract":[{"text":"A key aspect of bacterial survival is the ability to evolve while migrating across spatially varying environmental challenges. Laboratory experiments, however, often study evolution in well-mixed systems. Here, we introduce an experimental device, the microbial evolution and growth arena (MEGA)-plate, in which bacteria spread and evolved on a large antibiotic landscape (120 × 60 centimeters) that allowed visual observation of mutation and selection in a migrating bacterial front.While resistance increased consistently, multiple coexisting lineages diversified both phenotypically and genotypically. Analyzing mutants at and behind the propagating front,we found that evolution is not always led by the most resistant mutants; highly resistant mutants may be trapped behindmore sensitive lineages.TheMEGA-plate provides a versatile platformfor studying microbial adaption and directly visualizing evolutionary dynamics.","lang":"eng"}],"oa_version":"Preprint","issue":"6304","publication_status":"published","publication":"Science","title":"Spatiotemporal microbial evolution on antibiotic landscapes","scopus_import":1,"quality_controlled":"1","doi":"10.1126/science.aag0822","author":[{"last_name":"Baym","full_name":"Baym, Michael","first_name":"Michael"},{"last_name":"Lieberman","first_name":"Tami","full_name":"Lieberman, Tami"},{"last_name":"Kelsic","full_name":"Kelsic, Eric","first_name":"Eric"},{"id":"3464AE84-F248-11E8-B48F-1D18A9856A87","last_name":"Chait","orcid":"0000-0003-0876-3187","full_name":"Chait, Remy P","first_name":"Remy P"},{"last_name":"Gross","first_name":"Rotem","full_name":"Gross, Rotem"},{"last_name":"Yelin","full_name":"Yelin, Idan","first_name":"Idan"},{"last_name":"Kishony","first_name":"Roy","full_name":"Kishony, Roy"}],"_id":"1342","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5534434/","open_access":"1"}],"date_created":"2018-12-11T11:51:29Z","month":"09","citation":{"short":"M. Baym, T. Lieberman, E. Kelsic, R.P. Chait, R. Gross, I. Yelin, R. Kishony, Science 353 (2016) 1147–1151.","mla":"Baym, Michael, et al. “Spatiotemporal Microbial Evolution on Antibiotic Landscapes.” <i>Science</i>, vol. 353, no. 6304, American Association for the Advancement of Science, 2016, pp. 1147–51, doi:<a href=\"https://doi.org/10.1126/science.aag0822\">10.1126/science.aag0822</a>.","ama":"Baym M, Lieberman T, Kelsic E, et al. Spatiotemporal microbial evolution on antibiotic landscapes. <i>Science</i>. 2016;353(6304):1147-1151. doi:<a href=\"https://doi.org/10.1126/science.aag0822\">10.1126/science.aag0822</a>","ieee":"M. Baym <i>et al.</i>, “Spatiotemporal microbial evolution on antibiotic landscapes,” <i>Science</i>, vol. 353, no. 6304. American Association for the Advancement of Science, pp. 1147–1151, 2016.","chicago":"Baym, Michael, Tami Lieberman, Eric Kelsic, Remy P Chait, Rotem Gross, Idan Yelin, and Roy Kishony. “Spatiotemporal Microbial Evolution on Antibiotic Landscapes.” <i>Science</i>. American Association for the Advancement of Science, 2016. <a href=\"https://doi.org/10.1126/science.aag0822\">https://doi.org/10.1126/science.aag0822</a>.","ista":"Baym M, Lieberman T, Kelsic E, Chait RP, Gross R, Yelin I, Kishony R. 2016. Spatiotemporal microbial evolution on antibiotic landscapes. Science. 353(6304), 1147–1151.","apa":"Baym, M., Lieberman, T., Kelsic, E., Chait, R. P., Gross, R., Yelin, I., &#38; Kishony, R. (2016). Spatiotemporal microbial evolution on antibiotic landscapes. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.aag0822\">https://doi.org/10.1126/science.aag0822</a>"},"department":[{"_id":"CaGu"},{"_id":"GaTk"}],"oa":1,"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","date_published":"2016-09-09T00:00:00Z","day":"09","page":"1147 - 1151","status":"public","publisher":"American Association for the Advancement of Science","year":"2016"}