{"status":"public","title":"Multi-copper oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic redox-based reactions in roots","quality_controlled":"1","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_created":"2023-07-12T07:32:58Z","oa":1,"oa_version":"Published Version","publisher":"American Society of Plant Biologists","issue":"3","publication_status":"published","date_published":"2023-07-01T00:00:00Z","abstract":[{"text":"The primary cell wall is a fundamental plant constituent that is flexible but sufficiently rigid to support the plant cell shape. Although many studies have demonstrated that reactive oxygen species (ROS) serve as important signaling messengers to modify the cell wall structure and affect cellular growth, the regulatory mechanism underlying the spatial-temporal regulation of ROS activity for cell wall maintenance remains largely unclear. Here, we demonstrate the role of the Arabidopsis (Arabidopsis thaliana) multicopper oxidase-like protein skewed 5 (SKU5) and its homolog SKU5-similar 1 (SKS1) in root cell wall formation through modulating ROS homeostasis. Loss of SKU5 and SKS1 function resulted in aberrant division planes, protruding cell walls, ectopic deposition of iron, and reduced nicotinamide adeninedinucleotide phosphate (NADPH) oxidase-dependent ROS overproduction in the root epidermis–cortex and cortex–endodermis junctions. A decrease in ROS level or inhibition of NADPH oxidase activity rescued the cell wall defects of sku5 sks1 double mutants. SKU5 and SKS1 proteins were activated by iron treatment, and iron over-accumulated in the walls between the root epidermis and cortex cell layers of sku5 sks1. The glycosylphosphatidylinositol-anchored motif was crucial for membrane association and functionality of SKU5 and SKS1. Overall, our results identified SKU5 and SKS1 as regulators of ROS at the cell surface for regulation of cell wall structure and root cell growth.","lang":"eng"}],"department":[{"_id":"JiFr"}],"day":"01","file":[{"date_created":"2023-07-13T13:26:33Z","file_size":2076977,"file_id":"13220","success":1,"date_updated":"2023-07-13T13:26:33Z","file_name":"2023_PlantPhys_Chen.pdf","access_level":"open_access","creator":"cchlebak","content_type":"application/pdf","relation":"main_file","checksum":"5492e1d18ac3eaf202633d210fa0fb75"}],"type":"journal_article","tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"pmid":1,"date_updated":"2023-08-02T06:27:55Z","month":"07","article_processing_charge":"No","isi":1,"file_date_updated":"2023-07-13T13:26:33Z","acknowledgement":"We thank Dong liu for offering iron staining technique; ZhiChang Chen and Zhenbiao Yang for discussion; Dandan Zheng for earlier attempt; Liwen Jiang and Dingquan Huang for initial tests of the TEM experiment; John C. Sedbrook for a donation of sku5 and pSKU5::SKU5-GFP seeds; Catherine Perrot-Rechenmann and Ke Zhou for the donation of sks1, sks2, and sku5 sks1 seeds; Zengyu Liu and Zhongquan Lin for live-imaging microscopy assistance. We are grateful to Can Peng, and Xixia Li for helping with sample preparation, and taking TEM images, at the Center for Biological Imaging (CBI), Institute of Biophysics, Chinese Academy of Science.","ddc":["575"],"volume":192,"intvolume":"       192","year":"2023","citation":{"ama":"Chen C, Zhang Y, Cai J, et al. Multi-copper oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic redox-based reactions in roots. <i>Plant Physiology</i>. 2023;192(3):2243-2260. doi:<a href=\"https://doi.org/10.1093/plphys/kiad207\">10.1093/plphys/kiad207</a>","apa":"Chen, C., Zhang, Y., Cai, J., Qiu, Y., Li, L., Gao, C., … Gao, Z. (2023). Multi-copper oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic redox-based reactions in roots. <i>Plant Physiology</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1093/plphys/kiad207\">https://doi.org/10.1093/plphys/kiad207</a>","ista":"Chen C, Zhang Y, Cai J, Qiu Y, Li L, Gao C, Gao Y, Ke M, Wu S, Wei C, Chen J, Xu T, Friml J, Wang J, Li R, Chao D, Zhang B, Chen X, Gao Z. 2023. Multi-copper oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic redox-based reactions in roots. Plant Physiology. 192(3), 2243–2260.","chicago":"Chen, C, Y Zhang, J Cai, Y Qiu, L Li, C Gao, Y Gao, et al. “Multi-Copper Oxidases SKU5 and SKS1 Coordinate Cell Wall Formation Using Apoplastic Redox-Based Reactions in Roots.” <i>Plant Physiology</i>. American Society of Plant Biologists, 2023. <a href=\"https://doi.org/10.1093/plphys/kiad207\">https://doi.org/10.1093/plphys/kiad207</a>.","short":"C. Chen, Y. Zhang, J. Cai, Y. Qiu, L. Li, C. Gao, Y. Gao, M. Ke, S. Wu, C. Wei, J. Chen, T. Xu, J. Friml, J. Wang, R. Li, D. Chao, B. Zhang, X. Chen, Z. Gao, Plant Physiology 192 (2023) 2243–2260.","mla":"Chen, C., et al. “Multi-Copper Oxidases SKU5 and SKS1 Coordinate Cell Wall Formation Using Apoplastic Redox-Based Reactions in Roots.” <i>Plant Physiology</i>, vol. 192, no. 3, American Society of Plant Biologists, 2023, pp. 2243–60, doi:<a href=\"https://doi.org/10.1093/plphys/kiad207\">10.1093/plphys/kiad207</a>.","ieee":"C. Chen <i>et al.</i>, “Multi-copper oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic redox-based reactions in roots,” <i>Plant Physiology</i>, vol. 192, no. 3. American Society of Plant Biologists, pp. 2243–2260, 2023."},"article_type":"original","language":[{"iso":"eng"}],"_id":"13213","page":"2243-2260","doi":"10.1093/plphys/kiad207","has_accepted_license":"1","author":[{"full_name":"Chen, C","first_name":"C","last_name":"Chen"},{"last_name":"Zhang","first_name":"Y","full_name":"Zhang, Y"},{"last_name":"Cai","first_name":"J","full_name":"Cai, J"},{"full_name":"Qiu, Y","last_name":"Qiu","first_name":"Y"},{"full_name":"Li, L","last_name":"Li","first_name":"L"},{"last_name":"Gao","first_name":"C","full_name":"Gao, C"},{"last_name":"Gao","first_name":"Y","full_name":"Gao, Y"},{"full_name":"Ke, M","last_name":"Ke","first_name":"M"},{"first_name":"S","last_name":"Wu","full_name":"Wu, S"},{"full_name":"Wei, C","last_name":"Wei","first_name":"C"},{"full_name":"Chen, J","last_name":"Chen","first_name":"J"},{"full_name":"Xu, T","first_name":"T","last_name":"Xu"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml"},{"full_name":"Wang, J","first_name":"J","last_name":"Wang"},{"full_name":"Li, R","last_name":"Li","first_name":"R"},{"last_name":"Chao","first_name":"D","full_name":"Chao, D"},{"last_name":"Zhang","first_name":"B","full_name":"Zhang, B"},{"first_name":"X","last_name":"Chen","full_name":"Chen, X"},{"full_name":"Gao, Z","first_name":"Z","last_name":"Gao"}],"publication":"Plant Physiology","publication_identifier":{"issn":["0032-0889"],"eissn":["1532-2548"]},"external_id":{"pmid":["37010107"],"isi":["000971795800001"]}}