{"language":[{"iso":"eng"}],"_id":"12891","ec_funded":1,"citation":{"ieee":"A. Schauer, “Mesendoderm formation in zebrafish gastrulation: The role of extraembryonic tissues,” Institute of Science and Technology Austria, 2023.","mla":"Schauer, Alexandra. Mesendoderm Formation in Zebrafish Gastrulation: The Role of Extraembryonic Tissues. Institute of Science and Technology Austria, 2023, doi:10.15479/at:ista:12891.","short":"A. Schauer, Mesendoderm Formation in Zebrafish Gastrulation: The Role of Extraembryonic Tissues, Institute of Science and Technology Austria, 2023.","apa":"Schauer, A. (2023). Mesendoderm formation in zebrafish gastrulation: The role of extraembryonic tissues. Institute of Science and Technology Austria. https://doi.org/10.15479/at:ista:12891","ista":"Schauer A. 2023. Mesendoderm formation in zebrafish gastrulation: The role of extraembryonic tissues. Institute of Science and Technology Austria.","chicago":"Schauer, Alexandra. “Mesendoderm Formation in Zebrafish Gastrulation: The Role of Extraembryonic Tissues.” Institute of Science and Technology Austria, 2023. https://doi.org/10.15479/at:ista:12891.","ama":"Schauer A. Mesendoderm formation in zebrafish gastrulation: The role of extraembryonic tissues. 2023. doi:10.15479/at:ista:12891"},"publication_identifier":{"issn":["2663 - 337X"]},"doi":"10.15479/at:ista:12891","page":"190","author":[{"orcid":"0000-0001-7659-9142","first_name":"Alexandra","last_name":"Schauer","full_name":"Schauer, Alexandra","id":"30A536BA-F248-11E8-B48F-1D18A9856A87"}],"has_accepted_license":"1","ddc":["570"],"project":[{"call_identifier":"H2020","_id":"260F1432-B435-11E9-9278-68D0E5697425","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","grant_number":"742573"},{"grant_number":"25239","name":"Mesendoderm specification in zebrafish: The role of extraembryonic tissues","_id":"26B1E39C-B435-11E9-9278-68D0E5697425"}],"file_date_updated":"2023-05-05T13:04:15Z","year":"2023","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"department":[{"_id":"GradSch"},{"_id":"CaHe"}],"abstract":[{"text":"The tight spatiotemporal coordination of signaling activity determining embryo\r\npatterning and the physical processes driving embryo morphogenesis renders\r\nembryonic development robust, such that key developmental processes can unfold\r\nrelatively normally even outside of the full embryonic context. For instance, embryonic\r\nstem cell cultures can recapitulate the hallmarks of gastrulation, i.e. break symmetry\r\nleading to germ layer formation and morphogenesis, in a very reduced environment.\r\nThis leads to questions on specific contributions of embryo-specific features, such as\r\nthe presence of extraembryonic tissues, which are inherently involved in gastrulation\r\nin the full embryonic context. To address this, we established zebrafish embryonic\r\nexplants without the extraembryonic yolk cell, an important player as a signaling\r\nsource and for morphogenesis during gastrulation, as a model of ex vivo development.\r\nWe found that dorsal-marginal determinants are required and sufficient in these\r\nexplants to form and pattern all three germ layers. However, formation of tissues,\r\nwhich require the highest Nodal-signaling levels, is variable, demonstrating a\r\ncontribution of extraembryonic tissues for reaching peak Nodal signaling levels.\r\nBlastoderm explants also undergo gastrulation-like axis elongation. We found that this\r\nelongation movement shows hallmarks of oriented mesendoderm cell intercalations\r\ntypically associated with dorsal tissues in the intact embryo. These are disrupted by\r\nuniform upregulation of BMP signaling activity and concomitant explant ventralization,\r\nsuggesting that tight spatial control of BMP signaling is a prerequisite for explant\r\nmorphogenesis. This control is achieved by Nodal signaling, which is critical for\r\neffectively downregulating BMP signaling in the mesendoderm, highlighting that Nodal\r\nsignaling is not only directly required for mesendoderm cell fate specification and\r\nmorphogenesis, but also by maintaining low levels of BMP signaling at the dorsal side.\r\nCollectively, we provide insights into the capacity and organization of signaling and\r\nmorphogenetic domains to recapitulate features of zebrafish gastrulation outside of\r\nthe full embryonic context.","lang":"eng"}],"date_published":"2023-05-05T00:00:00Z","publication_status":"published","article_processing_charge":"No","supervisor":[{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"month":"05","date_updated":"2023-08-21T06:25:48Z","file":[{"checksum":"59b0303dc483f40a96a610a90aab7ee9","relation":"main_file","embargo_to":"open_access","creator":"aschauer","access_level":"closed","embargo":"2024-05-05","file_name":"Thesis_Schauer_final.pdf","content_type":"application/pdf","date_updated":"2023-05-05T13:01:14Z","file_size":31434230,"date_created":"2023-05-05T13:01:14Z","file_id":"12907"},{"content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","file_name":"Thesis_Schauer_final.docx","creator":"aschauer","access_level":"closed","relation":"source_file","checksum":"25f54e12479b6adaabd129a20568e6c1","file_id":"12908","date_created":"2023-05-05T13:04:15Z","file_size":43809109,"date_updated":"2023-05-05T13:04:15Z"}],"type":"dissertation","degree_awarded":"PhD","day":"05","alternative_title":["ISTA Thesis"],"title":"Mesendoderm formation in zebrafish gastrulation: The role of extraembryonic tissues","related_material":{"record":[{"status":"public","relation":"part_of_dissertation","id":"8966"},{"relation":"part_of_dissertation","id":"7888","status":"public"}]},"status":"public","publisher":"Institute of Science and Technology Austria","oa_version":"Published Version","date_created":"2023-05-05T08:48:20Z","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9"}