{"file_date_updated":"2023-01-23T08:56:21Z","isi":1,"acknowledgement":"We are grateful to N Barton, F Kondrashov, M Lagator, M Pleska, R Roemhild, D Siekhaus, and G\r\nTkacik for input on the manuscript and to K Tomasek for help with flow cytometry.","ddc":["570"],"volume":11,"intvolume":"        11","year":"2022","article_type":"original","citation":{"short":"I. Tomanek, C.C. Guet, ELife 11 (2022).","ama":"Tomanek I, Guet CC. Adaptation dynamics between copynumber and point mutations. <i>eLife</i>. 2022;11. doi:<a href=\"https://doi.org/10.7554/ELIFE.82240\">10.7554/ELIFE.82240</a>","apa":"Tomanek, I., &#38; Guet, C. C. (2022). Adaptation dynamics between copynumber and point mutations. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/ELIFE.82240\">https://doi.org/10.7554/ELIFE.82240</a>","chicago":"Tomanek, Isabella, and Calin C Guet. “Adaptation Dynamics between Copynumber and Point Mutations.” <i>ELife</i>. eLife Sciences Publications, 2022. <a href=\"https://doi.org/10.7554/ELIFE.82240\">https://doi.org/10.7554/ELIFE.82240</a>.","ista":"Tomanek I, Guet CC. 2022. Adaptation dynamics between copynumber and point mutations. eLife. 11, e82240.","ieee":"I. Tomanek and C. C. Guet, “Adaptation dynamics between copynumber and point mutations,” <i>eLife</i>, vol. 11. eLife Sciences Publications, 2022.","mla":"Tomanek, Isabella, and Calin C. Guet. “Adaptation Dynamics between Copynumber and Point Mutations.” <i>ELife</i>, vol. 11, e82240, eLife Sciences Publications, 2022, doi:<a href=\"https://doi.org/10.7554/ELIFE.82240\">10.7554/ELIFE.82240</a>."},"article_number":"e82240","_id":"12333","language":[{"iso":"eng"}],"author":[{"id":"3981F020-F248-11E8-B48F-1D18A9856A87","full_name":"Tomanek, Isabella","first_name":"Isabella","last_name":"Tomanek","orcid":"0000-0001-6197-363X"},{"orcid":"0000-0001-6220-2052","first_name":"Calin C","last_name":"Guet","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","full_name":"Guet, Calin C"}],"publication":"eLife","has_accepted_license":"1","doi":"10.7554/ELIFE.82240","publication_identifier":{"eissn":["2050-084X"]},"external_id":{"isi":["000912674700001"]},"status":"public","related_material":{"link":[{"relation":"software","url":"https://doi.org/10.5281/zenodo.6974122"}],"record":[{"status":"public","id":"12339","relation":"research_data"}]},"title":"Adaptation dynamics between copynumber and point mutations","quality_controlled":"1","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_created":"2023-01-22T23:00:55Z","oa_version":"Published Version","oa":1,"scopus_import":"1","publisher":"eLife Sciences Publications","date_published":"2022-12-22T00:00:00Z","publication_status":"published","abstract":[{"text":"Together, copy-number and point mutations form the basis for most evolutionary novelty, through the process of gene duplication and divergence. While a plethora of genomic data reveals the long-term fate of diverging coding sequences and their cis-regulatory elements, little is known about the early dynamics around the duplication event itself. In microorganisms, selection for increased gene expression often drives the expansion of gene copy-number mutations, which serves as a crude adaptation, prior to divergence through refining point mutations. Using a simple synthetic genetic reporter system that can distinguish between copy-number and point mutations, we study their early and transient adaptive dynamics in real time in Escherichia coli. We find two qualitatively different routes of adaptation, depending on the level of functional improvement needed. In conditions of high gene expression demand, the two mutation types occur as a combination. However, under low gene expression demand, copy-number and point mutations are mutually exclusive; here, owing to their higher frequency, adaptation is dominated by copy-number mutations, in a process we term amplification hindrance. Ultimately, due to high reversal rates and pleiotropic cost, copy-number mutations may not only serve as a crude and transient adaptation, but also constrain sequence divergence over evolutionary time scales.","lang":"eng"}],"department":[{"_id":"CaGu"}],"day":"22","type":"journal_article","file":[{"content_type":"application/pdf","creator":"dernst","access_level":"open_access","file_name":"2022_eLife_Tomanek.pdf","checksum":"9321fd5f06ff59d5e2d33daee84b3da1","relation":"main_file","success":1,"file_id":"12338","date_created":"2023-01-23T08:56:21Z","file_size":8835954,"date_updated":"2023-01-23T08:56:21Z"}],"date_updated":"2023-08-03T14:23:07Z","tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"month":"12","article_processing_charge":"No"}