{"external_id":{"isi":["000850348400025"]},"publication_identifier":{"issn":["2041-1723"]},"has_accepted_license":"1","author":[{"full_name":"Randriamanantsoa, S.","first_name":"S.","last_name":"Randriamanantsoa"},{"last_name":"Papargyriou","first_name":"A.","full_name":"Papargyriou, A."},{"full_name":"Maurer, H. C.","first_name":"H. C.","last_name":"Maurer"},{"last_name":"Peschke","first_name":"K.","full_name":"Peschke, K."},{"last_name":"Schuster","first_name":"M.","full_name":"Schuster, M."},{"last_name":"Zecchin","first_name":"G.","full_name":"Zecchin, G."},{"full_name":"Steiger, K.","last_name":"Steiger","first_name":"K."},{"first_name":"R.","last_name":"Öllinger","full_name":"Öllinger, R."},{"first_name":"D.","last_name":"Saur","full_name":"Saur, D."},{"full_name":"Scheel, C.","first_name":"C.","last_name":"Scheel"},{"full_name":"Rad, R.","last_name":"Rad","first_name":"R."},{"orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","first_name":"Edouard B"},{"first_name":"M.","last_name":"Reichert","full_name":"Reichert, M."},{"full_name":"Bausch, A. R.","last_name":"Bausch","first_name":"A. R."}],"publication":"Nature Communications","doi":"10.1038/s41467-022-32806-y","_id":"12217","article_number":"5219","language":[{"iso":"eng"}],"article_type":"original","citation":{"mla":"Randriamanantsoa, S., et al. “Spatiotemporal Dynamics of Self-Organized Branching in Pancreas-Derived Organoids.” <i>Nature Communications</i>, vol. 13, 5219, Springer Nature, 2022, doi:<a href=\"https://doi.org/10.1038/s41467-022-32806-y\">10.1038/s41467-022-32806-y</a>.","ieee":"S. Randriamanantsoa <i>et al.</i>, “Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids,” <i>Nature Communications</i>, vol. 13. Springer Nature, 2022.","ama":"Randriamanantsoa S, Papargyriou A, Maurer HC, et al. Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids. <i>Nature Communications</i>. 2022;13. doi:<a href=\"https://doi.org/10.1038/s41467-022-32806-y\">10.1038/s41467-022-32806-y</a>","chicago":"Randriamanantsoa, S., A. Papargyriou, H. C. Maurer, K. Peschke, M. Schuster, G. Zecchin, K. Steiger, et al. “Spatiotemporal Dynamics of Self-Organized Branching in Pancreas-Derived Organoids.” <i>Nature Communications</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s41467-022-32806-y\">https://doi.org/10.1038/s41467-022-32806-y</a>.","apa":"Randriamanantsoa, S., Papargyriou, A., Maurer, H. C., Peschke, K., Schuster, M., Zecchin, G., … Bausch, A. R. (2022). Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-022-32806-y\">https://doi.org/10.1038/s41467-022-32806-y</a>","ista":"Randriamanantsoa S, Papargyriou A, Maurer HC, Peschke K, Schuster M, Zecchin G, Steiger K, Öllinger R, Saur D, Scheel C, Rad R, Hannezo EB, Reichert M, Bausch AR. 2022. Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids. Nature Communications. 13, 5219.","short":"S. Randriamanantsoa, A. Papargyriou, H.C. Maurer, K. Peschke, M. Schuster, G. Zecchin, K. Steiger, R. Öllinger, D. Saur, C. Scheel, R. Rad, E.B. Hannezo, M. Reichert, A.R. Bausch, Nature Communications 13 (2022)."},"ec_funded":1,"year":"2022","intvolume":"        13","volume":13,"project":[{"grant_number":"851288","_id":"05943252-7A3F-11EA-A408-12923DDC885E","name":"Design Principles of Branching Morphogenesis","call_identifier":"H2020"}],"ddc":["570"],"file_date_updated":"2023-01-27T08:14:48Z","acknowledgement":"A.R.B. acknowledges the financial support of the European Research Council (ERC) through the funding of the grant Principles of Integrin Mechanics and Adhesion (PoINT) and the German Research Foundation (DFG, SFB 1032, project ID 201269156). E.H. was supported by the European Union (European Research Council Starting Grant 851288). D.S., M.R., and R.R. acknowledge the support by the German Research Foundation (DFG, SFB1321 Modeling and Targeting Pancreatic Cancer, Project S01, project ID 329628492). C.S. and M.R. acknowledge the support by the German Research Foundation (DFG, SFB1321 Modeling and Targeting Pancreatic Cancer, Project 12, project ID 329628492). M.R. was supported by the German Research Foundation (DFG RE 3723/4-1). A.P. and M.R. were supported by the German Cancer Aid (Max-Eder Program 111273 and 70114328).\r\nOpen Access funding enabled and organized by Projekt DEAL.","isi":1,"keyword":["General Physics and Astronomy","General Biochemistry","Genetics and Molecular Biology","General Chemistry","Multidisciplinary"],"month":"09","article_processing_charge":"No","date_updated":"2023-08-04T09:25:23Z","tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"type":"journal_article","file":[{"relation":"main_file","checksum":"295261b5172274fd5b8f85a6a6058828","content_type":"application/pdf","file_name":"2022_NatureCommunications_Randriamanantsoa.pdf","access_level":"open_access","creator":"dernst","date_updated":"2023-01-27T08:14:48Z","file_id":"12416","success":1,"file_size":22645149,"date_created":"2023-01-27T08:14:48Z"}],"day":"05","department":[{"_id":"EdHa"}],"abstract":[{"text":"The development dynamics and self-organization of glandular branched epithelia is of utmost importance for our understanding of diverse processes ranging from normal tissue growth to the growth of cancerous tissues. Using single primary murine pancreatic ductal adenocarcinoma (PDAC) cells embedded in a collagen matrix and adapted media supplementation, we generate organoids that self-organize into highly branched structures displaying a seamless lumen connecting terminal end buds, replicating in vivo PDAC architecture. We identify distinct morphogenesis phases, each characterized by a unique pattern of cell invasion, matrix deformation, protein expression, and respective molecular dependencies. We propose a minimal theoretical model of a branching and proliferating tissue, capturing the dynamics of the first phases. Observing the interaction of morphogenesis, mechanical environment and gene expression in vitro sets a benchmark for the understanding of self-organization processes governing complex organoid structure formation processes and branching morphogenesis.","lang":"eng"}],"date_published":"2022-09-05T00:00:00Z","publication_status":"published","publisher":"Springer Nature","scopus_import":"1","oa_version":"Published Version","oa":1,"date_created":"2023-01-16T09:46:53Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","quality_controlled":"1","title":"Spatiotemporal dynamics of self-organized branching in pancreas-derived organoids","status":"public","related_material":{"record":[{"relation":"research_data","id":"13068","status":"public"}]}}