{"year":"2016","publist_id":"6113","volume":5,"intvolume":"         5","ddc":["581"],"project":[{"_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants","grant_number":"282300","call_identifier":"FP7"}],"acknowledgement":"This work was supported by ERC Independent Research grant (ERC-2011-StG-20101109-PSDP to JF). JM internship was supported by the grant “Action Austria – Slovakia”. MG was supported by the scholarship \"Stipendien der Stipendienstiftung der Republik Österreich\". Work by EH and CPR were supported by ANR blanc ANR-14-CE11-0018. We would like to thank Mark Estelle and Yunde Zhao for provid\r\n-\r\ning \r\nabp1-c1\r\n, \r\nabp1-TD1 \r\nand \r\nabp1-WTc1 \r\nseeds. We thank Emeline \r\nHuault for technical assistance.","file_date_updated":"2020-07-14T12:44:39Z","doi":"10.12688/f1000research.7654.1","publication":"F1000 Research ","author":[{"first_name":"Jaroslav","last_name":"Michalko","id":"483727CA-F248-11E8-B48F-1D18A9856A87","full_name":"Michalko, Jaroslav"},{"orcid":"0000-0003-0619-7783","full_name":"Glanc, Matous","id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2","first_name":"Matous","last_name":"Glanc"},{"first_name":"Catherine","last_name":"Perrot Rechenmann","full_name":"Perrot Rechenmann, Catherine"},{"last_name":"Friml","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596"}],"has_accepted_license":"1","pubrep_id":"711","language":[{"iso":"eng"}],"article_number":"86","_id":"1221","ec_funded":1,"article_type":"original","citation":{"ieee":"J. Michalko, M. Glanc, C. Perrot Rechenmann, and J. Friml, “Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein,” <i>F1000 Research </i>, vol. 5. F1000 Research, 2016.","mla":"Michalko, Jaroslav, et al. “Strong Morphological Defects in Conditional Arabidopsis Abp1 Knock-down Mutants Generated in Absence of Functional ABP1 Protein.” <i>F1000 Research </i>, vol. 5, 86, F1000 Research, 2016, doi:<a href=\"https://doi.org/10.12688/f1000research.7654.1\">10.12688/f1000research.7654.1</a>.","short":"J. Michalko, M. Glanc, C. Perrot Rechenmann, J. Friml, F1000 Research  5 (2016).","apa":"Michalko, J., Glanc, M., Perrot Rechenmann, C., &#38; Friml, J. (2016). Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein. <i>F1000 Research </i>. F1000 Research. <a href=\"https://doi.org/10.12688/f1000research.7654.1\">https://doi.org/10.12688/f1000research.7654.1</a>","ista":"Michalko J, Glanc M, Perrot Rechenmann C, Friml J. 2016. Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein. F1000 Research . 5, 86.","chicago":"Michalko, Jaroslav, Matous Glanc, Catherine Perrot Rechenmann, and Jiří Friml. “Strong Morphological Defects in Conditional Arabidopsis Abp1 Knock-down Mutants Generated in Absence of Functional ABP1 Protein.” <i>F1000 Research </i>. F1000 Research, 2016. <a href=\"https://doi.org/10.12688/f1000research.7654.1\">https://doi.org/10.12688/f1000research.7654.1</a>.","ama":"Michalko J, Glanc M, Perrot Rechenmann C, Friml J. Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein. <i>F1000 Research </i>. 2016;5. doi:<a href=\"https://doi.org/10.12688/f1000research.7654.1\">10.12688/f1000research.7654.1</a>"},"scopus_import":"1","publisher":"F1000 Research","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2018-12-11T11:50:47Z","oa":1,"oa_version":"Published Version","status":"public","title":"Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein","quality_controlled":"1","tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"date_updated":"2022-03-24T09:12:49Z","article_processing_charge":"No","month":"01","day":"20","file":[{"file_name":"IST-2016-711-v1+1_770cf1e0-612f-4e85-a500-54b6349fbbab_7654_-_jaroslav_michalko.pdf","creator":"system","access_level":"open_access","content_type":"application/pdf","relation":"main_file","checksum":"c9e50bb6096a7ba4a832969935820f19","file_size":2990459,"date_created":"2018-12-12T10:15:33Z","file_id":"5154","date_updated":"2020-07-14T12:44:39Z"}],"type":"journal_article","abstract":[{"lang":"eng","text":"The Auxin Binding Protein 1 (ABP1) is one of the most studied proteins in plants. Since decades ago, it has been the prime receptor candidate for the plant hormone auxin with a plethora of described functions in auxin signaling and development. The developmental importance of ABP1 has recently been questioned by identification of Arabidopsis thaliana abp1 knock-out alleles that show no obvious phenotypes under normal growth conditions. In this study, we examined the contradiction between the normal growth and development of the abp1 knock-outs and the strong morphological defects observed in three different ethanol-inducible abp1 knock-down mutants ( abp1-AS, SS12K, SS12S). By analyzing segregating populations of abp1 knock-out vs. abp1 knock-down crosses we show that the strong morphological defects that were believed to be the result of conditional down-regulation of ABP1 can be reproduced also in the absence of the functional ABP1 protein. This data suggests that the phenotypes in abp1 knock-down lines are due to the off-target effects and asks for further reflections on the biological function of ABP1 or alternative explanations for the missing phenotypic defects in the abp1 loss-of-function alleles."}],"department":[{"_id":"JiFr"}],"date_published":"2016-01-20T00:00:00Z","publication_status":"published"}